光声成像技术简介课件

“”光声成像技术简介•1901inPhysics:Roentgenfordiscoveryofx-raysin1895•1953inPhysics:Zernikeforinventionofphasecontrastopticalmicroscopy•1979inMedicine:CormackandHounsfieldforinventionofCTinearly1970s•2003inMedicine:LauterburandMansfieldforinventionofMRIinearly1970s•2008inChemistry:OsamuShimomura,MartinChalfieandRogerY.Tsienfordiscoveryanddevelopmentofgreenfluorescentprotein•2014inChemistry:Eric

Betzig,WilliamMoerner,andStefanHellforsuper-resolvedfluorescencemicroscopyNobelPrizesRelatedtoBiomedicalImagingComparisonofvariousimagingmodalities研究动机..Safety—Non-ionizingradiation:photonenergyis~2eV.Noradioactive..Physics—Relatedtothemolecularconformationoftissue...Optics—Highintrinsiccontrast:..Opticalabsorption:Angiogenesis,hyper-metabolism,apoptosis,necrosis,andexogenouscontrastagents...Opticalscattering:Sizeofcellnuclei...Opticalpolarization:Collagen,musclefibers...研究动机Physiology—Functionalimagingofphysiologicalparameters:..Oxygensaturationofhemoglobin..Totalhemoglobinconcentration(relatedtobloodvolume)..Enlargementofcellnuclei..Orientationofcollagen..Denaturationofcollagen..Bloodflow(Doppler)Physiology—Molecularimaging(exogenouscontrastagents)...….研究动机OpticalfrequencyshiftsduetotheopticalDopplereffectprovideinformationaboutbloodflow.Opticalpropertiesoftargetedcontrastagentsprovidecontrastforthemolecularimagingofbiomarkers.Opticalpropertiesorbioluminescenceofproductsfromgeneexpressionprovidecontrastforthemolecularimagingofgeneactivities.Opticalspectroscopypermitssimultaneousdetectionofmultiplecontrastagents.Opticaltransparencyintheeyeprovidesauniqueopportunityforhigh-resolutionimagingoftheretina.光子在生物体内轨迹Laser1mmCFM&2PMOCTDOT,PAT散射（扩散现象）限制弹道成像渗透到〜1mm的皮肤。被光声成像(PAT)克服

实现超深度的高分辨成像。技术创新点UltrasoundtransducerstoreceivesignalsProducedultrasonicwaves(~mbar)Laserormicrowavepulse

(ANSIlimit)光声成像基本原理AlexanderG.Bell’sPhotophone（历史）[1]AGBell,“Ontheproductionandreproductionofsoundbylight,”AmJScience20,305,1880[2]“Productionofsoundbyradiantenergy,”ManufacturerandBuilder13,156,1881Photoacousticeffect:LightabsorptionTemperatureriseThermoelasticexpansionAcousticemission光的吸收比尔定理血液吸收系数血液吸收系数乳腺组织的穿透深度黑色素吸收系数脂肪吸收系数亚甲基兰吸收曲线深度与分辨率比值ADRRof100orgreaterisconsideredtoindicatehighresolutionintermsofpixelcount.光声技术表现形式光声成像范围光声技术表现形式：PACTPATPAT光声技术表现形式：球形扫描成像血管增生多元阵列探测器多元阵列探测器临床乳腺癌成像光声无损穿颅（人骨）成像光声可以成功穿透1cm厚成人头骨国外已开展临床婴幼儿脑出血光声检测成像光声内窥镜光声内窥镜光声内窥镜微血管成像AfterGOinjection,thevascularnetworkwithdefinedarchitecturewasclearlyvisible微血管成像L.Nieetal,ACSNano,8(12),2014毛细血管和血红细胞(RBC)进行成像CapillarybedRBCs50µm500µm微血管成像Earlystageimagingofchemotherapyresponse疗效监测L.Nieetal,ACSNano,8(12),2014药物的代谢过程与器官分布药代动力学L.Nie*etal,Biomed.Opt.Express,5(8):2679-2685.(2014).全身成像多尺度光声成像：器官—组织—细胞—细胞器小鼠全身成像EY,eyes;CV,corticalvessels;LV,liver;VC,venacava;GI,GItract;KN,kidney;PV,portalvein;SC,spinalcord;SP,spleen.应用目标(从临床前延伸至临床)：早期肿瘤检测与诊断如皮肤癌、乳腺癌等血管畸形、血管瘤检测成像实时手术导航治疗效果的疗效监测病理学分析VascularmalformationPort-wineStainMalignantmelanomaNormal

BreastCancerSkinCancerBreastCancerMicroPET/CT(SiemensInveon)MicroMRI(Bruker9.4