行情磷酸化丝裂原活化蛋白激酶1-2抗体简介

中文名称磷酸化丝裂原活化蛋白激酶1/2抗体英文名称phospho-ERK1+2(Thr183/185)别????名ERK1+ERK2(phosphoThr183/Thr185);phospho-ERK1/MAPK-1/2(Thr183/185);ERK1;ERK2;ERK-2;ERK1;ERK2;ERT1;ERT2;Extracellularsignalregulatedkinase1;Extracellularsignalregulatedkinase1;Extracellularsignalregulatedkinase2;Extracellularsignalregulatedkinase2;Extracellularsignal-regulatedkinase2;HS44KDAP;HUMKER1A;InsulinstimulatedMAP2kinase;MAPkinase1;MAPkinase2;MAPkinaseisoformp42;MAPkinaseisoformp44;MAPK1;MAPK2;MAPK1;MAPK2;MGC20180;Microtubuleassociatedprotein2kinase;Mitogenactivatedproteinkinase1;Mitogenactivatedproteinkinase1;Mitogenactivatedproteinkinase2;Mitogen-activatedproteinkinase1;Mitogen-activatedproteinkinase2;MK01_MOUSE;p38;p40;p41;p41mapk;p42MAPK;p42-MAPK;p42MAPK;p42MAPK;p44ERK1;p44MAPK;p44ERK1;p44ERK1;p44MAPK;p44MAPK;PRKM1;PRKM1;PRKM2;PRKM2;PRKM1;PRKM2;Proteinkinasemitogenactivated1;Proteinkinasemitogenactivated1;Proteinkinasemitogenactivated2;Proteinkinasemitogenactivated2;Proteintyrosinekinas.供应商远慕生物产品类型磷酸化抗体研究领域免疫学??神经生物学??信号转导??干细胞??激酶和磷酸酶抗体来源Rabbit克隆类型Polyclonal交叉反应Human,?Mouse,?Rat,?Chicken,?Dog,?Cow,?Horse,?Rabbit,?GuineaPig,产品应用WB=1:100-500?ELISA=1:500-1000?IP=1:20-100?IHC-P=1:100-500?IHC-F=1:100-500?IF=1:100-500?磷酸化丝裂原活化蛋白激酶1/2抗体（石蜡切片需做抗原修复）?notyettestedinotherapplications.optimaldilutions/concentrationsshouldbedeterminedbytheenduser.分?子?量41kDa性????状LyophilizedorLiquid浓????度1mg/1ml免?疫?原KLHconjugatedSynthesisedphosphopeptidederivedfrommouseERK1aroundthephosphorylationsiteofThr183/185亚????型IgG纯化方法affinitypurifiedbyProteinA储?存?液0.01MPBS,pH7.4with10mg/mlBSAand0.1%Sodiumazide保存条件Storeat-20°Cforoneyear.Avoidrepeatedfreeze/thawcycles.Thelyophilizedantibodyisstableatroomtemperatureforatleastonemonthandforgreaterthanayearwhenkeptat-20°C.WhenreconstitutedinsterilepH7.40.01MPBSordiluentofantibodytheantibodyisstableforatleasttwoweeksat2-4°C.磷酸化丝裂原活化蛋白激酶1/2抗体产品介绍background:Mitogen-activatedproteinkinase(MAPK)signalingcascadesincludeMAPKorextracellularsignal-regulatedkinase(ERK),MAPKkinase(MKKorMEK),andMAPKkinasekinase(MAPKKKorMEKK).MAPKKkinase/MEKKphosphorylatesandactivatesitsdownstreamproteinkinase,MAPKkinase/MEK,whichinturnactivatesMAPK.Thekinasesofthesesignalingcascadesarehighlyconserved,andhomologsexistinyeast,Drosophila,andmammaliancells.MAPKKK5contains1,374aminoacidswithall11kinasesubdomains.NorthernblotanalysisshowsthatMAPKKK5transcriptisabundantlyexpressedinhumanheartandpancreas.TheMAPKKK5proteinphosphorylatesandactivatesMKK4(aliasesSERK1,MAPKK4)invitro,andactivatesc-JunN-terminalkinase(JNK)/stress-activatedproteinkinase(SAPK)duringtransientexpressioninCOSand293cells;MAPKKK5doesnotactivateMAPK/ERK.[providedbyRefSeq,Jul2008]Function:Serine/threoninekinasewhichactsasanessentialcomponentoftheMAPkinasesignaltransductionpathway.MAPK1/ERK2andMAPK3/ERK1arethe2MAPKswhichplayanimportantroleintheMAPK/ERKcascade.TheyparticipatealsoinasignalingcascadeinitiatedbyactivatedKITandKITLG/SCF.Dependingonthecellularcontext,theMAPK/ERKcascademediatesdiversebiologicalfunctionssuchascellgrowth,adhesion,survivalanddifferentiationthroughtheregulationoftranscription,translation,cytoskeletalrearrangements.TheMAPK/ERKcascadeplaysalsoaroleininitiationandregulationofmeiosis,mitosis,andpostmitoticfunctionsindifferentiatedcellsbyphosphorylatinganumberoftranscriptionfactors.About160substrateshavealreadybeendiscoveredforERKs.Manyofthesesubstratesarelocalizedinthenucleus,andseemtoparticipateintheregulationoftranscriptionuponstimulation.However,othersubstratesarefoundinthecytosolaswellasinothercellularorganelles,andthoseareresponsibleforprocessessuchastranslation,mitosisandapoptosis.Moreover,theMAPK/ERKcascadeisalsoinvolvedintheregulationoftheendosomaldynamics,includinglysosomeprocessingandendosomecyclingthroughtheperinuclearrecyclingcompartment(PNRC);aswellasinthefragmentationoftheGolgiapparatusduringmitosis.Thesubstratesincludetranscriptionfactors(suchasATF2,BCL6,ELK1,ERF,FOS,HSF4orSPZ1),cytoskeletalelements(suchasCANX,CTTN,GJA1,MAP2,MAPT,PXN,SORBS3orSTMN1),regulatorsofapoptosis(suchasBAD,BTG2,CASP9,DAPK1,IER3,MCL1orPPARG),regulatorsoftranslation(suchasEIF4EBP1)andavarietyofothersignaling-relatedmolecules(likeARHGEF2,DCC,FRS2orGRB10).Proteinkinases(suchasRAF1,RPS6KA1/RSK1,RPS6KA3/RSK2,RPS6KA2/RSK3,RPS6KA6/RSK4,SYK,MKNK1/MNK1,MKNK2/MNK2,RPS6KA5/MSK1,RPS6KA4/MSK2,MAPKAPK3orMAPKAPK5)andphosphatases(suchasDUSP1,DUSP4,DUSP6orDUSP16)areothersubstrateswhichenablethepropagationtheMAPK/ERKsignaltoadditionalcytosolicandnucleartargets,therebyextendingthespecificityofthecascade.MediatesphosphorylationofTPRinresponstoEGFstimulation.Mayplayaroleinthespindleassemblycheckpoint.PhosphorylatesPMLandpromotesitsinteractionwithPIN1,leadingtoPMLdegradation(Bysimilarity).[FUNCTION]Actsasatranscriptionalrepressor.Bindstoa[GC]AAA[GC]consensussequence.Represstheexpressionofinterferongamma-inducedgenes.SeemstobindtothepromoterofCCL5,DMP1,IFIH1,IFITM1,IRF7,IRF9,LAMP3,OAS1,OAS2,OAS3andSTAT1.Transcriptionalactivityisindependentofkinaseactivity(Bysimilarity).?Subunit:Bindsbothupstreamactivatorsanddownstreamsubstratesinmultimolecularcomplexes.InteractswithADAM15,ARHGEF2,ARRB2,DAPK1(viadeathdomain),HSF4,IER3,IPO7,DUSP6,NISCH,SGK1,andisoform1ofNEK2.Interacts(viaphosphorylatedform)withTPR(viaC-terminusregionandphosphorylatedform);theinteractionrequiresdimerizationofMAPK1/ERK2andincreasesfollowingEGFstimulation(Bysimilarity).Interacts(phosphorylatedform)withCAV2('Tyr-19'-phosphorylatedform);theinteraction,promotedbyinsulin,leadstonuclearlocationandMAPK1activation(Bysimilarity).InteractswithDCC(Bysimilarity).InteractswithMORG1,PEA15andMKNK2.MKNK2isoform1bindingpreventsfromdephosphorylationandinactivation.ThephosphorylatedforminteractswithPML(Bysimilarity).SubcellularLocation:retention.Phosphorylationspindle(Bysimilarity).Nucleus.Cytoplasm,cytoskeleton,centrosome(Bysimilarity).Cytoplasm.