Trolox-DataSheet-生命科学试剂-MedChemExpress

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemETroloxCat.No.:HY-101445CASNo.:53188-07-1分⼦式:C₁₄H₁₈O₄分⼦量:250.29作⽤靶点:ReactiveOxygenSpecies;Ferroptosis;Apoptosis作⽤通路:Immunology/Inflammation;MetabolicEnzyme/Protease;NF-κB;Apoptosis储存⽅式:Powder-20°C3years4°C2yearsInsolvent-80°C6months-20°C1month溶解性数据体外实验DMSO:≥100mg/mL(399.54mM)*"≥"meanssoluble,butsaturationunknown.MassSolvent1mg5mg10mgConcentration制备储备液1mM3.9954mL19.9768mL39.9537mL5mM0.7991mL3.9954mL7.9907mL10mM0.3995mL1.9977mL3.9954mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存⽅式和期限：-80°C,6months;-20°C,1month。-80°C储存时，请在6个⽉内使⽤，-20°C储存时，请在1个⽉内使⽤。体内实验请根据您的实验动物和给药⽅式选择适当的溶解⽅案。以下溶解⽅案都请先按照InVitro⽅式配制澄的储备液，再依次添加助溶剂：(为保证实验结果的可靠性，澄的储备液可以根据储存条件，适当保存；体内实验的⼯作液，建议您现⽤现配，当天使⽤；以下溶剂前显⽰的百分⽐指该溶剂在您配制终溶液中的体积占⽐；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的⽅式助溶)1/3MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemE1.请依序添加每种溶剂：10%DMSO>>40%PEG300>>5%Tween-80>>45%salineSolubility:≥2.08mg/mL(8.31mM);Clearsolution2.请依序添加每种溶剂：10%DMSO>>90%(20%SBE-β-CDinsaline)Solubility:≥2.08mg/mL(8.31mM);Clearsolution3.请依序添加每种溶剂：10%DMSO>>90%cornoilSolubility:≥2.08mg/mL(8.31mM);ClearsolutionBIOLOGICALACTIVITY⽣物活性Trolox⼀种维⽣素E的类似物，具有强⼤的抗氧化作⽤。Trolox还⼀种强⼤的膜损伤抑制剂。体外研究Troloxhasshowntoprotectmammaliancellsfromoxidativedamage.Troloxiseffectiveinpreventingmyocytenecrosisincellculturestudiesandinacaninemodeloftwohoursofleftanteriordescendingcoronaryartery(LAD)occlusionfollowedbyfourhoursofreperfusion[1].Troloxcouldpreventoxidativestress-inducedapoptosisinthymocytes.Pre-orposttreatmentofcellswithTroloxreducedH2O2-inducedDNAfragmentationtocontrollevelsandbelow.[2].Troloxisahydrophilicanalogueofalpha-tocopherolandreportedtoscavengeperoxylradicalsbetterthanvitaminEinsodiumdodecylsulfatemicellesandinliposomes.Troloxprolongssubstantiallythesurvivalofhumanventricularmyocytesandhepatocyteagainstoxyradicalsgeneratedwithxanthineoxidaseplushypoxanthine,andpreventedlysisofredcellsexposedtoanazo-initiator[3].PROTOCOLCellAssay[1]ThymocytesaretreatedwithTrolox(10mM)before,during,orafter10minexposureto10μMH2O2.TroloxincubationsthatareconductedbeforeorafterH2O2exposuresareperformedinTCM.TroloxtreatmentsthatareadministeredconcurrentlywithH2O2areperformedinPBSwithferroussulfate[1].MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly.户使⽤本产品发表的科研⽂献•CellRep.2022Oct11;41(2):111462.•CellRep.2022Sep20;40(12):111381.•CellDeathDis.2021Apr1;12(4):338.•OxidMedCellLongev.2020Jan27;2020:5829521.•CellDeathDiscov.2022May2;8(1):238.Seemorecustomervalidationsonwww.MedChemEREFERENCES[1].MickleDA,etal.Myocardialsalvagewithtroloxandascorbicacidforanacuteevolvinginfarction.AnnThoracSurg.1989Apr;47(4):553-2/3MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemE7.[2].ForrestVJ,etal.Oxidativestress-inducedapoptosispreventedbyTrolox.FreeRadicBiolMed.1994Jun;16(6):675-84.[3].WuTW,etal.ThecytoprotectiveeffectofTroloxdemonstratedwiththreetypesofhumancells.BiochemCellBiol.1990Oct;68(10):1189-94.McePdfHeightCaution:Producthasnotbeen