临床医生如何做科研 选题与实例

临床医生如何做科研？

实例：简单条件下科研选题及实施

临床医生为何做科研1）需要,Theneedofresearchpapersforpromotionetc.shouldNOTbeagoodmotivation,butitisareality.2）兴趣,Interestisabetterdriveforresearch,andyoucanseekforcollaborationoncehaveanidea,evenwhenyoudonothaveresourcestodoso.3）永生,Forthoseexpertclinicianswhohaveuniqueandeffectivetherapies,whomayhavethestrongestmotivationtohavetheirexpertisevalidatedviaorganizedclinicaltrialsandpublished,andthusbeingpermanentlyrecordedandre-produced.如何做科研？

…useavailableconditions1.如何选出A+(最可行)的主题

1).文献复习(PubMed,+GoogleScholar) 2).结合实际(现有条件),寻找最好的材料

3).初步探索，观察现象或治疗效果4).设计相应基础研究探讨或确定机制

1)文献复习(PubMed,GoogleScholar)

1.SearchPubMedusing:keyword-1ANDkeyword-2…etc;2.Orsimplytypeyourthoughtingooglesearch例1:研究依据活性氧自由基（ReactiveOxygenSpecies,ROS）介导的脂质过氧化被认为是缺血再灌注损伤（Ischemia-ReperfusionInjury,IRI）的主要机制，但临床抗氧化治疗IRI效果不佳15-F2t-isoprostane,一种ROS所致过氧化损伤的特异性标记,并且在心肌缺血/再灌注损伤中起作用

科研假设：阻断15-F2t-isoprostane的形成或功能应该能减弱缺血后心肌的损伤和功能障碍

例1:

首先观察

[15-F2t-isop]在心脏手术病人心肌缺血和再灌注中不同时点改变情况，及与手术后心功能的关系Ansley,XiaandDhaliwal,JThoracCardiovascSurg2003n=30提出假说（Hypothesis）Blockadeofformationoractionsof15-F2tisoprostaneshouldattenuatepost-ischemicmyocardialdysfunction制定动物实验方案1

（ExperimentalApproach）Langendorffset-upIsolatedheartsfromyoungmalerats(250-300g)subjectedto40minglobalischemiaand90minreperfusionConstantflowperfusion(10ml/min)Duringischemia,salinewasinfusedat60l/minviaaorta制定动物实验方案2DrugApplicationProtocol

KH20’10’60’75’150’KHorDrug40min

ischemia(Salineordrug)Drug10’20’60’150’A.Measure

LVMechanics

CoronaryPressure0’10’50’60.5’

65’90’150’

B.Sample

EffluentProtocoldiagramKHKH分析结果1：PropofolDecreases[15-F2t-isop]

DuringIschemiaandReperfusionXiaZ（夏正远）etal.CanJPhysiolPharmacol2003Propofol分析结果2：HighdosePropofolIncreasedheartantioxidantcapacity,enhancedmyocardialeffectivecontraction(有效收缩,LVDP）XiaZ（夏正远）etal.CanJPhysiolPharmacol2003推广演讲：MaintainaBalance(SafetyIssues)--HighdosePropofol安全性SightseeingRideabike(enjoyingandprotectingenvironment)orDriveaBMW(saferandbetterview??)Trytofilleachbasketwithoneofthenumbersbetween1and12,tomakeitbalanced由前述动物研究，回到临床试验：HighDosePropofolAttenuatesPost-ischemicMyocardialInjuryinPatientsWithDurationofIschemia60Min:ComparisonWithIsofluraneXiaZ，HuangZetal,Anesth

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