Necrosulfonamide-DataSheet-生命科学试剂-MedChemExpress

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemENecrosulfonamideCat.No.:HY-100573CASNo.:1360614-48-7分⼦式:C₁₈H₁₅N₅O₆S₂分⼦量:461.47作⽤靶点:MixedLineageKinase作⽤通路:MAPK/ERKPathway储存⽅式:Powder-20°C3years4°C2yearsInsolvent-80°C6months-20°C1month溶解性数据体外实验DMSO:≥28mg/mL(60.68mM)*"≥"meanssoluble,butsaturationunknown.MassSolvent1mg5mg10mgConcentration制备储备液1mM2.1670mL10.8349mL21.6699mL5mM0.4334mL2.1670mL4.3340mL10mM0.2167mL1.0835mL2.1670mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存⽅式和期限：-80°C,6months;-20°C,1month。-80°C储存时，请在6个⽉内使⽤，-20°C储存时，请在1个⽉内使⽤。体内实验请根据您的实验动物和给药⽅式选择适当的溶解⽅案。以下溶解⽅案都请先按照InVitro⽅式配制澄的储备液，再依次添加助溶剂：(为保证实验结果的可靠性，澄的储备液可以根据储存条件，适当保存；体内实验的⼯作液，建议您现⽤现配，当天使⽤；以下溶剂前显⽰的百分⽐指该溶剂在您配制终溶液中的体积占⽐；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的⽅式助溶)1.请依序添加每种溶剂：10%DMSO>>40%PEG300>>5%Tween-80>>45%saline1/2MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemESolubility:2.5mg/mL(5.42mM);Suspendedsolution;Needultrasonic2.请依序添加每种溶剂：10%DMSO>>90%(20%SBE-β-CDinsaline)Solubility:2.5mg/mL(5.42mM);Suspendedsolution;Needultrasonic3.请依序添加每种溶剂：10%DMSO>>90%cornoilSolubility:2.5mg/mL(5.42mM);Suspendedsolution;Needultrasonic4.请依序添加每种溶剂：20%SBE-β-CDinsalineSolubility:6.67mg/mL(14.45mM);Suspendedsolution;Needultrasonic5.请依序添加每种溶剂：50%PEG300>>50%salineSolubility:10mg/mL(21.67mM);Suspendedsolution;NeedultrasonicBIOLOGICALACTIVITY⽣物活性Necrosulfonamide，⼀种necroptosis抑制剂，通过选择性靶向(MLKL)。Necrosulfonamide阻⽌MLKL-RIP1-RIP3坏死⼩体复合体与其下游效应⼦相互作⽤。MLKL诱导坏死过程中RIP3的重底物。体外研究NecrosulfonamidespecificallyblocksnecrosisdownstreamofRIP3activation.NecrosulfonamideinhibitsMLKL-mediatednecrosisbyblockingItsN-terminalCCdomainfunction[1].户使⽤本产品发表的科研⽂献•Circulation.2022Nov30.•JClinInvest.2021Nov9;e151268.•NatCommun.2022Nov17;13(1):7031.•Autophagy.2021Jul20;1-19.•JExpClinCancerRes.2021Jun23;40(1):206.Seemorecustomervalidationsonwww.MedChemEREFERENCES[1].SunL,etal.Mixedlineagekinasedomain-likeproteinmediatesnecrosissignalingdownstreamofRIP3kinase.Cell.2012;148(1-2):213-227.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearchuse