Ionomycin-SQ23377-DataSheet-生命科学试剂-MedChemExpress

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemEIonomycinCat.No.:HY-13434CASNo.:56092-81-0Synonyms:SQ23377分⼦式:C₄₁H₇₂O₉分⼦量:709.01作⽤靶点:CalciumChannel;PKC;Bacterial;Apoptosis;Antibiotic作⽤通路:MembraneTransporter/IonChannel;NeuronalSignaling;Epigenetics;TGF-beta/Smad;Anti-infection;Apoptosis储存⽅式:Solution,-20°C,2years*Insolvent:-80°C,6months;-20°C,1month(2years)溶解性数据体外实验DMSO:100mg/mL(141.04mM;Needultrasonic)Ethanol:100mg/mL(141.04mM;Needultrasonic)MassSolvent1mg5mg10mgConcentration制备储备液1mM1.4104mL7.0521mL14.1042mL5mM0.2821mL1.4104mL2.8208mL10mM0.1410mL0.7052mL1.4104mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存⽅式和期限：-80°C,6months;-20°C,1month。-80°C储存时，请在6个⽉内使⽤，-20°C储存时，请在1个⽉内使⽤。体内实验请根据您的实验动物和给药⽅式选择适当的溶解⽅案。以下溶解⽅案都请先按照InVitro⽅式配制澄的储备液，再依次添加助溶剂：(为保证实验结果的可靠性，澄的储备液可以根据储存条件，适当保存；体内实验的⼯作液，建议您现⽤现配，当天使⽤；以下溶剂前显⽰的百分⽐指该溶剂在您配制终溶液中的体积占⽐；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的⽅式助溶)1.请依序添加每种溶剂：10%DMSO>>40%PEG300>>5%Tween-80>>45%saline1/3MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemESolubility:≥2.5mg/mL(3.53mM);Clearsolution2.请依序添加每种溶剂：10%DMSO>>90%(20%SBE-β-CDinsaline)Solubility:≥2.5mg/mL(3.53mM);Clearsolution3.请依序添加每种溶剂：10%DMSO>>90%cornoilSolubility:≥2.5mg/mL(3.53mM);Clearsolution4.请依序添加每种溶剂：10%EtOH>>90%cornoilSolubility:≥2.5mg/mL(3.53mM);ClearsolutionBIOLOGICALACTIVITY⽣物活性Ionomycin(SQ23377)⼀种有效的，选择性的钙离⼦载体(calciumionophore)，也⼀种由Streptomycesconglobatus产⽣的抗⽣素。Ionomycin(SQ23377)对⼆价阳离⼦具有⾼度特异性(Ca>Mg>Sr=Ba)。Ionomycin(SQ23377)促进细胞凋亡。Ionomycin(SQ23377)还诱导蛋⽩激酶C(PKC)激活。IC50&TargetCalciumionophore[1]体外研究IonomycinisaCalciumionophoreandanantibioticproducedbyStreptomycesconglobatus[1].Additionof2μMIonomycintoLCLC103HcellscausesaninstantaneousincreaseinintracellularCa2+concentrationfrom50to180nM.DNAandproteinanalysisinIonomycin-treatedculturesrevealedDNAfragmentationandPARPcleavagetoan85-kDafragmenttypicalofcaspase-mediatedapoptosis.Necrosiscouldbedetectedin~1-5%oftheIonomycintreatedcells.CaspaseactivationinwholecellswasfollowedbymonitoringtheincreaseinactivityagainstAc-DEVD-amcfollowingIonomycintreatment[2].Ionomycin-mediatedcleavageandexosomerelease.FollowingIonomycinexposure,mediumconditionedbySKOV3ipcellshadincreasedamountsofexosomescontainingtheL1-32cleavagefragment[4].Ionomycinalsophosphorylatep38MAPKbyCa2+influxthroughSOCE,leadingtosuppressionofTNF-α-inducedNF-κBphosphorylation[5].户使⽤本产品发表的科研⽂献•CellMolImmunol.2022Feb22.•MolCell.2022May5;S1097-2765(22)00327-6.•SciTranslMed.2020Nov25;12(571):eaaz6667.•ProteinCell.2021Oct22;1-21.•CancerCommun(Lond).2022Jan3.Seemorecustomervalidationsonwww.MedChemEREFERENCES[1].JunsukeUwada,etal.Store-operatedcalciumentry(SOCE)contributestophosphorylationofp38MAPKandsuppressionofTNF-αsignallingintheintestinalepithelialcells.CellSignal.2019Nov;63:109358.[2].LiuC,etal.Characterizationofionomycinasacalciumionophore.JBiolChem.1978Sep10;253(17):5892-4.2/3MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemE[3].ChatilaT,etal.MechanismsofTcellactivationbythecalciumionophoreionomycin.JImmunol.1989Aug15;143(4):1283-9.[4].Gil-ParradoS,etal.Ionomycin-activatedcalpaintriggersapoptosis.AprobableroleforBcl-2familymembers.JBiolChem.2002Jul26;277(30):27217-26.[5].StoeckA,etalAroleforexosomesintheconstitutiveandstimulus-inducedectodomaincleavageofL1andCD44.BiochemJ.2006Feb1;393(Pt3):609-18.McePdfHeightCaution:Pro