教程教案讲稿acquity qda detector overview and maintenance guiderev

ACQUITYQDaOverviewandMaintenanceCopyright©WatersCorporation2013sGeneral©2013WATERSCORPORATION.PRINTEDINTHEUNITEDSTATESOFAMERICAANDINIRELAND. .THISORPARTSTHEREOFMAYNOTBEREPRODUCEDINANYFORMWITHOUTTHEWRITTENPERMISSIONOFTHEPUBLISHER.Theinformationinthis issubjecttochangewithoutnoticeandshouldnotbeconstruedasacommitmentbyWatersCorporation.WatersCorporationassumesnoresponsibilityforanyerrorsthatmayappearin.This isbelievedtobecompleteandaccurateatthetimeofpublication.InnoeventshallWatersCorporationbeliableforincidentalorconsequentialdamagesinconnectionwith,orarisingfrom,itsuse.Forthemostrecentrevisionofthis ,consulttheWatersWebsite POSSIBLE.”,andWatersQualityParts,aresofWatersCorporation.QDaandRADARaretrademarksofWatersCorporation.PEEKisaofVictrexManufacturing,TygonisaofSaint-GobainVitonisaofDupontDowOthersortrademarksarethesolepropertyoftheirWaters’TechnicalCommunicationsorganizationinvitesyoutoreportanyerrorsthatyouencounterinthis ortosuggestideasforotherwiseimprovingit.HelpusbetterunderstandwhatyouexpectfromourationsothatwecancontinuouslyimproveitsaccuracyWeseriouslyconsidereverycustomercommentwereceive.Youcanreachus ContactWaters®withenhancementrequestsortechnicalquestionsregardingtheuse,transportation,removal,ordisposalofanyWatersproduct.YoucanreachusviatheInternet, ephone,orconventionalmail.TheWatersWebsiteincludescontactinformationforWaterslocationsworldwide.ephoneandFromtheUSAorCanada,phone800252-4752,orfax5088721990.Forotherlocationsworldwide,phoneandfaxnumbersappearintheWatersWebsite.34MapleStreetMilford,MASafetySomereagentsandsamplesusedwithWatersinstrumentsanddevicescanposechemical,biological,orradiologicalhazards(oranycombinationthereof).Youmustknowthepotentiallyhazardouseffectsofallsubstancesyouworkwith.AlwaysfollowGoodLaboratoryPractice,andconsultyourorganization’sstandardoperatingprocedures.ConsiderationsspecifictotheACQUITYQDaThesourceexhaustsystemisdesignedtoberobustandleak-tight.Watersmendsyouperforma ysis,assuming umleakthelaboratoryatmosphereof10%LCeluate,withanadditional0.5%leakfortheStandardinstrument.Warning:Toavoidleaksofmaterialsthatarebiohazardousortoxins,followtheseguidelines:Toconfirmtheintegrityofthesourceexhaustsystem,renewthesourceO-ringsatintervalsnotexceedingoneyear.ToavoidchemicaldegradationofthesourceO-rings,whichcanwithstandexposureonlytocertainsolvents(seepage76),determinewhetheranysolventsyouusethatarenotlistedarechemicallycompatiblewiththecompositionoftheO-rings.Warning:Topreventignitionofflammablesolventvaporsintheenclosedspaceofamassspectrometer’sionsource,ensurethatnitrogenflowscontinuouslythroughthesource.Thenitrogensupplypressuremustnotfallbelow600kPa(6.0bar,87psi)duringan requiringtheuseofflammablesolvents.Warning:Toavoidburninjuries,avoidtouchingthesourceenclosurewhenoperatingorservicingtheinstrument.SourceenclosureHighvoltageToavoidelectricshock,donotremovethemassprotectivepanels.ThecomponentstheycoverarenotToavoidnonlethalelectricshockwhentheinstrumentisswitchedon,avoidtouchingtheareasmarkedwiththehighvoltagewarningsymbol.Totouchthoseareas,firstswitchtheinstrumentoff.HazardsassociatedwithremovinganinstrumentfromWarning:Toavoid alcontaminationwithbiohazards,toxicmaterials,andcorrosivematerials,wearchemical-resistantglovesduringallphasesofinstrumentWarning:Toavoidpunctureinjuries,handlesyringes,fusedsilicalines,andborosilicatetipswithextremecare.Whenyouremovetheinstrumentfromusetorepairordisposeofit,youmustdecontaminateallofitsvacuumareas.Thesearetheareasinwhichyoucanexpecttoencounterthehighestlevelsofcontamination:SourceWasteExhaustRotarypumpoil(whereTheneedtodecontaminateothervacuumareasoftheinstrumentdependsonthekindsofsamplestheinstrument yzedandtheirlevelsofconcentration.DonotdisposeoftheinstrumentorreturnittoWatersforrepairuntiltheauthorityresponsibleforapprovingitsremovalfromthepremisesspecifiestheextentofdecontaminationrequiredandthelevelofresidualcontaminationpermissible.ThatauthoritymustalsoprescribethemethodofdecontaminationtobeusedandtheappropriateprotectionfornelundertakingthedecontaminationYoumusthandleitemssuchassyringes,fusedsilicalines,andborosilicatetipsusedtocarrysampleintothesourceareainaccordancewithlaboratoryproceduresforcontaminatedvesselsandsharps.Toavoidcontaminationbycarcinogens,toxicsubstances,orbiohazards,youmustwearchemical-resistantgloveswhenhandlingordisposingofuse FCCradiationemissionsChangesormodificationsnotexpresslyapprovedbythepartyresponsibleforcompliance,couldvoidtheusersauthoritytooperatetheequipment.ThisdevicecomplieswithPart15oftheFCCRules.Operationissubjecttothe oconditions:(1)thisdevicemaynotcauseharmfulinterference,and(2)thisdevicemustacceptanyinterferencereceived,includinginterferencethatmaycauseundesiredoperation.CanadaspectrummanagementemissionsThisclassAdigitalproductapparatuscomplieswithCanadianICES-003.CetappareilnumériquedelaclasseAestconformeàlanormeNMB-ElectricalpowersafetyDonotpositiontheinstrumentsothatitisdifficulttodisconnectthepowerSafetyhazardsymbolationneedstobeconsultedinallcaseswherethesymbolisusedtofindoutthenatureofthepotentialhazardandanyactionswhichhavetobetaken.EquipmentmisuseIftheequipmentisusedinamannernotspecifiedbythemanufacturer,theprotectionprovidedbytheequipmentmaybeimpaired.SafetyConsultAppendixAforacomprehensivelistofwarningandcautionOperatingthisWhenoperatingthisinstrument,followstandardquality-control(QC)proceduresandtheguidelinespresentedinthissection.ApplicableAuthorizedrepresentativeoftheEuropeanConfirmsthatamanufacturedproductcomplieswithallapplicableEuropeanCommunityConfirmsthatamanufacturedproductcomplieswithallapplicableUnitedStatesandCanadiansafetyrequirementsElectricalandelectronicequipmentwiththissymbolmaycontainhazardoussubstancesandshouldnotbedisposedofasgeneralwaste.ForcompliancewiththeWasteElectricalandElectronicEquipmentDirective(WEEE)2012/19/EU,contactWatersCorporationforthecorrectdisposalandrecyclinginstructions.AudienceandThisguideisfornoviceusers,andassumesnoknowledgeofliquidchromatographyormassspectrometryprinciples.Itprovidesanoverviewoftheinstrumentandexplainshowtoinstallit,prepareitforoperation,andmaintainit.IntendeduseoftheACQUITYQDaWatersdesignedtheACQUITYQDaDetectorforuseasanionandtationtool,aspartofanACQUITYUPLCorUPC2system.TheACQUITYQDaDetectorisnotintendedfor gnosticTocalibra Csystems,followacceptablecalibrationmethodsusingatleastfivestandardstogenerateastandardcurve.TheconcentrationrangeforstandardsshouldincludetheentirerangeofQCsamples,typicalspecimens,andatypicalspecimens.Quality-RoutinelyrunthreeQCsamplesthatrepresentsubnormal,normal,andabove-normallevelsofacompound.EnsurethatQCsampleresultsfallwithinanacceptablerange,andevaluateprecisionfromdaytodayandruntorun. ollectedwhenQCsamplesareoutofrangemightnotbevalid.DonotreportthesedatauntilyouarecertainthattheinstrumentperformsISMISMClassification:ISMGroup1ClassThisclassificationhasbeenassignedinaccordancewithIECCISPR11IndustrialScientificandMedical(ISM)instrumentsrequirements.Group1productsapplytointentionallygeneratedand/orusedconductivelycoupledradio-frequencyenergythatisnecessaryfortheinternalfunctioningoftheClassAproductsaresuitableforuseinallestablishmentsotherthanresidentiallocationsandthosedirectlyconnectedtoalowvoltage,power-supplynetworksupplyingabuildingfordomesticpurposes.Theremaybedifficultiesinensuringelectromagneticcompatibilityinotherenvironmentsduetoconductedaswellasradiateddisturbances.ECauthorizedWatersCorporation(MicromassUK FloatsRoadManchesterM239LZUnitedKingdom TableofGeneral Copyright Customer Contacting Safety ConsiderationsspecifictotheACQUITYQDa FCCradiationemissions Safetyhazardsymbol Safety Audienceand IntendeduseoftheACQUITYQDa Quality- ISM ISMClassification:ISMGroup1Class ECauthorized InstrumentfeaturesandOperating Operating Ion Auto Sample Vacuum Preparingfor Rearpanel Connectingtotheelectricity Startingthe Maintenance Spare Replacing TroubleshootingusingConnections Safetyand Removingandrefittingthesource Removingthesourceenclosurefromthe Fittingthesourceenclosuretothe Maintainingthesource Removingthesampleconeassemblyfromtheion Replacingtheentrance-aperturesealand Removingtheion Cleaningthesource Refittingthesource Cleaningtheionguide Removingtheionguideassemblyfromthesource Cleaningthedifferential Cleaningtheionguide Fittingtheionguideassemblytothe Emptyingthenitrogenexhaust Replacingtheroughingpump’s ASafety Warning Specific Caution WarningsthatapplytoallWatersinstrumentsand Warningsthataddressthereplacingof Electricalandhandling Electrical Handling B Physical Environmental Electrical Input/output CMaterialsofConstructionandCompatible Preventing Itemsexposedto Solventsusedtopreparemobile DExternal Externalwiringandvacuum ConnectingtheStandardinstrument’sbacking ConnectingthePerformanceinstrument’sbacking Makingtheelectricalconnectionstotherotary Connectingtothenitrogengas Connectingthenitrogenexhaust Connectingtheexhaustsolenoid Connectingthe ConnectingEthernet Input/outputsignal Signal Connectingtothepower InstrumentfeaturesandOperatingModesToeffectivelyusetheACQUITY®QDa™Detector,youmustfamiliarizeyourselfwithitsoperatingmodesandfeature. Ion Auto Sample Vacuum YoucanacquiredatausinganyoftheseoperatingScanning,wheretheinstrumentscansacrossauser-definedspanofmass-to-charge(m/z)ratiostoproduceamassspectrum.Selectedionrecording(SIR),wheretheinstrumentrecordsthesignalintensityatastaticm/zratioforthepurposeoftation.Multiplem/zratioscanberecordedsimultaneously.MSe,wheretheinstrumentperformsbothlowandhighfragmentationenergyscansinasingleruntoaidincompoundidentification.RADAR,wheretheinstrumentperformsScanningandSIRacquisitionsImportant:Donotopentheinstrument’sfrontdoorduringdataacquisition.ngsocancelstheacquisition.IonIonsflowthroughtheinstrumentinthefollowingSamplesfromtheLCareintroducedintotheionizationTheionspassthroughthesampleconeintothevacuumTheionspassthroughthetransferoptics(theionguides)tothequadrupole,wheretheyarefilteredaccordingtotheirm/zratios.ThetransmittedionsaredetectedbythephotomultiplierdetectionThesignalisamplified,digitized,andsenttothesoftware AutoAutoTheinstrumentcanperformautosetupcheckswhenitpowerson.Ifautosetupisenabled,theinstrumentperformsastartupcheckofmassscalecalibrationandquadrupole(spectrum)resolution,usinganinternalcalibrantduringstartup.Iftheinstrumentreportsnoproblems,thestatusLEDshowsgreen,andtheinstrumentisreadyforuse.Ifthecheckidentifiesaproblem,thesoftwarepromptsyoutostartanautomaticcorrectionprocess.Thequadrupoleresolutionandmassscalecalibrationc sobecheckedondemandwithoutpoweringofftheinstrument.Tolearnhowtoenablestartupchecks,seetheinstrument’sonlineSampleConnecttheACQUITYQDatotheprecedinginstrumentinyoursystemusingthedprobeassembly.Youcanuse250-mmor500-mmprobeassemblies,whicheverbestsuitsyourconfiguration.Ifrequired,useauniontoextendthelengthtofitwithyourconfiguration.mendation:Wherepossible,usethe250-mmPEEKtubing. ngsominimizesbandbroadening.Caution:Ensuresuitabletubingisusedtopreventthepressureexceedingthelimitsoftheprecedinginstruments.Aninternalturbomolecularpumpandexternalbackingpumpcreateinstrument’svacuum.ThePerformanceinstrumentusesaseparaterotaryvanepump,andtheStandardinstrumentusesadiaphragmpumpattachedtothebackoftheinstrument.Vacuumleaks,electricalfailures,andvacuumpumpfailurescausevacuumloss,thedamagefromwhichispreventedbyprotectiveinterlocks.Thesystemmonitorsturbomolecularpumpspeedandcontinuouslymeasuresturbopower.Theturbospeedalsoservesasaswitch,stopoperationwhenitsensesvacuumloss.PreparingforPreparethedetectorforuseaccordingtothespecificationsinthis Rearpanel Connectingtotheelectricity Startingthe RearpanelWhenmakingconnectionstotheQDadetector’srearpanel,refertothediagraminAppendixD,“ExternalConnections”.Fordetailsofsupportedinletsystemconfigurations,contactWatersTechnicalConnectingtotheelectricityTheACQUITYQDarequiresaseparate,grounded(earthed)electricitysource.Thegroundconnectionintheelectricaloutletmustbecommonandconnectednearthesystem.Warning:Toavoidelectricalshock,observetheseUsetheSVT-typepowercordintheUnitedStatesandtheHAR-type(orbetter)inEurope.Themainpowercordmustbereplacedwithoneofadequaterating.Tolearnwhichcordtouseinothercountries,contactyourlocalWaters®Power-offandunplugthedetectorbeforeperforminganymaintenanceontheinstrument.ConnectallcomponentsoftheACQUITYinletsystemtoacommonToconnecttotheelectricitymendation:Usealineconditioneranduninterruptiblepowersupply(UPS)foroptimumlong-terminputvoltage.Connectthefemaleendofthepowercordtothereceptacleontherearpanelofthedetector.ConnectthemaleendofthepowercordtoasuitablewallAlternative:IfyoursystemincludestheoptionalFlexCart,connectthefemaleendofthecart’selectricalcable(includedinthestartupkit)tothereceptacleontherearpaneloftheinstrument.Connectthehooded,maleendofthecart’selectricalcabletothepowerstriponthebackofthecart.Finally,connectthepowerstrip’scabletoawalloutletoperatingonitsowncircuit.StartingtheStartingtheWarning:Toavoidelectricshockandburninjuries,followtheseDonotexposethedatasystemoranyancillaryequipmenttodriporsplashingliquids.Donotplaceobjectsfilledwithliquid,suchassolventbottles,onthedatasystemorancillaryequipment.Caution:Toavoiddamagetotheinstrumentcausedby solvents,refertoAppendixC,“MaterialsofConstructionandCompatibleSolvents”.Startingtheinstrumententailspowering-ontheACQUITYworkstation,loggingintotheworkstation,powering-ontheinstrumentandallotherACQUITYinstruments,andstartingthesoftware.Requirement:Youmustpower-onandlogintotheACQUITYUPLCworkstationfirsttoensurethatitobtainstheIPaddressesofthesystemTostarttheWarning:Toavoidignitingflammablesolvents,neverletthenitrogensupplypressurefallbelow600kPa(6.0bar,87psi).Ontherearpanel,ensurethenitrogensupplyisconnectedtoinstrument’snitrogeninletconnection(seepageRequirement:Thenitrogenmustbedryan l-free,withapurityofatleast95%.Regulatethesupplyat600to690kPa(6.0to6.9bar,87to100Power-ontheworkstation,andlogPressthepowerswitchesonthetop,left-handsidesoftheACQUITYsystemmodules,includingtheQDadetector.Result: oduleestablishescommunicationswiththeAllowapproxima y5minutesfortheinstrumentstoestablishTip:ThepowerandstatusLEDsshowsteadygreenwhentheinstrumentshaveestablishedcommunications.Ontheworkstation,starttheInthesoftware,monitortheInstrumentConsoleformessagesandLEDKeeptoamaintenanceschedule,andperformmaintenanceasrequiredanddescribedinthischapter. Spare Replacing Safetyand Removingandrefittingthesource Maintainingthesource Cleaningtheionguide Emptyingthenitrogenexhaust Replacingtheroughingpump’s Toensurethatyoursystemoperatesasdesigned,useonlyWatersParts®.Visit forinformationaboutWatersQualityParts,includinghowtoorderthem.ReplacingWarning:Toprotectagainstfirehazard,replacefuseswiththoseofthetypeandratingspecifiedinthefollowingtable,andprintedonthepanelsadjacenttotheinstrument’sfuseWarning:Toavoidelectricshock,disconnectthemassspectrometerfromthepowersupplybeforereplacingfuses.Theinstrumenthastwofuses,andusesdoublepole/neutralfusing.Circuitscanremainliveevenwhenonefusehasblown.Ifeitheroftheinstrument'sfuses,locatedontherearpanel,ruptureoreotherwisefaulty,replaceitwithafuseofthistypeandrating.FuseRear5×T8H250ConnectionsINSIGHT®isan“in ligent”devicemanagement(IDM)WebservicethatenablesWaterstoprovideproactiveserviceandsupportfortheACQUITYUPLCsystem.TouseConnectionsINSIGHT,youmustinstallitsserviceagentsoftwareonyourMassLynxworkstation.Inaclient/serversystem,youmustalsoinstalltheserviceagent,onthecomputerfromwhichyoucontrolthesystem.TheserviceagentsoftwareautomaticallyandsecurelycapturesandsendsinformationaboutthesupportneedsofyoursystemdirectlytoWaters.IfyouencounteraperformanceproblemwhenusingtheInstrumentConsole,youcanmanuallysubmitaConnectionsInsightrequesttoWaterscustomersupport.Alternatively,youcanuseRemoteDesktop,areal-timecollaborationoptionthatcontrolsthetwo-wayconnectionwiththeACQUITYUPLCsystembyenablingtheConnectionsINSIGHTiAssistservicelevel.ConsultthesesourcesformoreinformationaboutConnectionsINSIGHTandConnectionsINSIGHTiAssist:ConnectionsINSIGHTInstallationGuide(partnumberConnectionsINSIGHTUser'sGuide(partnumberYoursalesYourlocalWatersWatersCustomerTosubmitaConnectionsInsightSelectTroubleshoot>SubmitConnectionsInsightIntheConnectionsInsightRequestdialogbox,typeyourname,ephonenumber, address,andadescriptionoftheproblem.ClickSubmit,andallowapproxima y5minutestosavetheserviceResult:A.zipfilecontainingyourConnectionsInsightprofileisforwardedtoWaterscustomersupportforreview.Tip:SavingaserviceprofileorplotfilefromtheInstrumentConsolecanrequireasmuchas150MBoffilespace.SafetyandBearinmindthefollowingsafetyconsiderationswhenperformingmaintenanceprocedures:Warning:Theinstrumentcomponentscanbecontaminatedwithbiohazardsortoxicmaterials.Alwayswearchemical-resistant,powder-freegloveswhilehandlingthecomponents.Warning:Topreventinjury,followtheseAlwaysobserveGoodLaboratoryPracticewhenhandlingsolvents,changingtubing,oroperatingtheinstrument.Knowthephysicalandchemicalpropertiesofthesolventsused(seetheMaterialSafetyDataSheetsforthesolventsinuse).Ensuretheinstrumentisfullyventedbeforeperformingmaintenanceprocedures.Warning:Toavoidelectricdonotremovetheinstrument’spanels,fortheycovernouser-serviceableitems.ensurethattheinstrumentisinStandbymodebeforeyoubeginanymaintenanceoperation.Warning:Theprobeandsourcecanbehot.Toavoidburninjuries,takegreatcarewhileworkingwiththesecomponents.Caution:Whenperformingmaintenanceoperationsinsidethesourceenclosure,ensurethatthesecriteriaaremet:TheLCflowisSeeAppendixAforsafetyadvisoryRemovingandrefittingthesourceRemovingandrefittingthesourceRemovethesourceenclosuretogainaccesstothesourcecomponentsthatneedroutinecleaningandreplacement.RemovingthesourceenclosurefromtheWarning:Thesourcecomponentscanbecontaminatedwithbiohazardsortoxicmaterials.Alwayswearchemical-resistant,powder-freegloveswhileperformingthisCaution:Toavoiddamagingthefragileprobe,usecarewhenremovingitfromthesourceenclosure.RequiredChemical-resistant,powder-freeToremovethesourcePower-offtheinstrumentusingthepowerbuttoninthetop,left-handcornerofthefrontpanel.Waitfor y5minutes,toallowtheinstrumenttoOpenthesourceenclosureTodisconnectthesourceenclosure’selectricalcablefromthefrontoftheinstrument,loosenthescrewsandpullthecablefromthesocket.Loosenthe2thumbscrewsonthefrontofthesourceWarning:Toavoidburninjuries,allowthesourceenclosuretocoolbeforetouchingthemetalinterior.Toremovethesourceenclosure,pullitawayfromtheinstrumentusingbothhands.Warning:Toavoidpunctureinjuriesfromthesharpprobetip,usecarewheninsertingandremovingtheprobefromthesourceRemovetheprobefromthetopofthesourceenclosureusingtheFittingthesourceenclosuretotheRequiredChemical-resistant,powder-freeWarning:Toavoid alcontaminationwithbiohazardsortoxicmaterials,wearchemical-resistant,powder-freegloveswhileperformingthisprocedure.TofitthesourceUsingbothhands,slidethesourceenclosureontosupportingTosecuretheenclosureagainsttheinstrument,tightenthe2thumbscrewsonthefrontofthesourceenclosure.Caution:Toavoiddamagetotheelectricalconnector’sscrews,donotovertightenthem.Connecttheelectricalcabletothesocketontheright-handsideofinstrument’sfrontpanel,andtightentheCaution:Toavoiddamagingthefragileprobe,usecarewheninsertingitintothesourceenclosure.CarefullyinserttheprobeintotheorificeatthetopofthesourceTosecuretheprobe,tightenthePEEKscrewatopthesourceTip:ThescrewclickswhenitisfullyMaintainingthesourceCleanthesourcecomponentswhentheseconditionsThesampleconeandconegasnozzlearevisiblyYouhavedismissedinletandsample-relatedcausesfordecreasedsignalRemovingthesampleconeassemblyfromtheionRequiredChemical-resistant,powder-freeWarning:Toavoid alcontaminationwithbiohazardsortoxicmaterials,alwayswearchemical-resistant,Warning:Toavoidburninjuries,allowtheionblocktocoolbeforeperformingthisprocedure.ToremovethesourcecomponentsfromtheionRemovethesourceenclosure(seepageToremovethegasconeandPEEKconeclamp,pulltheconeclampawayfromtheinstrument.PEEKconeIonRemovingthegasconeandPEEKconeIonGasRemovethesampleconeassemblyfromtheright-handsideoftheionblock,anddisassemblethepartsbyhand.Fourpartscomposethesampleconeassembly:ametalsamplecone;ablack,rubberentrance-apertureseal;ametalentrance-aperturedisc,foundinsidetheentrance-apertureseal;ametalentrance-aperturecarrier.Therubberentranceaperturesealandmetalentance-aperturediscareconsumables,providedassembledasasinglepart.Requirement:Whenorderingareplacemententrance-aperturediscandseal,ensuretheentrance-aperturediscisthecorrectsizeforyourQDamodel.IfyourQDaisaStandardinstrument,orderthe0.1-mmdisc.IfyourQDaisaPerformanceinstrument,orderthe0.2-mmdisc.Removingthesamplecone

Ion SampleconeSampleconeassemblySample

Replacingtheentrance-aperturesealandReplacetheentrancesealaperturewhentheseconditionsYouhavedismissedLCandsample-relatedcausesfordecreasedsignalCleaningthesourcecomponentsfailstoincreasesignalRequiredChemical-resistant,powder-freeWarning:Toavoid alcontaminationwithbiohazardsortoxicmaterials,alwayswearchemical-resistant,powder-freegloveswhileperformingthisprocedure.Disposeoftheoldentrance-aperturesealanddiscaccordingtoyourlocalenvironmentalregulations.Insertthenewentrance-aperturesealanddiscintothesampleRequirement:Whenorderingareplacemententrance-aperturediscandseal,ensuretheentrance-aperturediscisthecorrectsizeforyourQDamodel.IfyourQDaisaStandardinstrument,orderthe0.1-mmdisc.IfyourQDaisaPerformanceinstrument,orderthe0.2-mmdisc.Refitthesourcecomponents(seepageRemovingtheionCleantheionblockwhencleaningthesampleconeassemblyfailstoincreasesignalsensitivity.RequiredChemical-resistant,powder-free2.5-mmAllenWarning:Toavoid alcontaminationwithbiohazardsortoxicmaterials,alwayswearchemical-resistant,Warning:Toavoidpunctureinjuriesfromthesharpcalibrationpin,usecarewhenremovingitfromandreplacingitintheionToremovetheionRemovethecalibrationpinfromthetopoftheionblockbypullingitTip:Tolocatethecalibrationpin,seethediagramonpageRemovethesampleconeassemblyfromtheright-handsideoftheionblock(seepage31).Removethe2screwssecuringtheionblocktotheinstrumentusingthe2.5-mmAllenwrench,andremovetheionblock.Ionblocksecuring RequiredChemical-resistant,powder-free ysizedglassvesselsinwhichtocomple yimmersecomponentswhencleaning.Useonlyglasswarenotpreviouslycleanedwithsurfactants.FormicSourceofoil-free,inertgas(nitrogenorargon)fordrying(air-dryingWashbottlecontainingHPLC-grade(orbetter)1:1LargeWarning:Toavoid alcontaminationwithbiohazardsortoxicmaterials,alwayswearchemical-resistant,powder-freegloveswhileperformingthisprocedure.Warning:Toavoidcontaminationwithcorrosiveandtoxicformicacid,useafumehoodandsuitableprotectiveequipmentduringthisprocedure.TocleanthesourceRemoveanyO-ringsfromthesourcecomponents,andremovetheinsulatorforthePEEKcalibrationpinfromthetopoftheionblock.Immersethesamplecone,entrance-aperturecarrier,andionblockinseparateglassvesselscontaining1:1methanol/water.Note:Donotcleantherubberentrance-aperturesealentrance-aperturedisc.Instead,replacetheseconsumableswhentheyarevisiblydamaged.Tip:Ifthecomponentsareobviouslycontaminated,use45:45:10methanol/water/formicacid.Placethevesselsintheultrasonicbathfor30Ifyouusedformicacidinthecleaningsolution,doasRinsethecomponentsbyimmersingtheminseparateglassvesselscontainingwater,andthenplacethevesselsintheultrasonicbathfor20minutes.Removeanyresidualwaterfromthecomponentsbyimmersingtheminglassvesselscontainingmethanol,andthenplacethevesselsintheultrasonicbathfor10minutes.Caution:Toavoidrecontaminatingthecomponents,wearclean,chemical-resistant,powder-freeglovesfortherestofthisCarefullyremovethecomponentsfromthevessels,andblow-drythemwithinert,oil-freegas.Inspecteachcompo