HCoV-229E-IN-1-DataSheet-生命科学试剂-MedChemExpress

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemEHCoV-229E-IN-1Cat.No.:HY-132169分⼦式:C₃₈H₅₃N₃O₂分⼦量:583.85作⽤靶点:SARS-CoV作⽤通路:Anti-infection储存⽅式:Powder-20°C3years4°C2yearsInsolvent-80°C6months-20°C1month溶解性数据体外实验DMSO:12.5mg/mL(21.41mM;Needultrasonic)MassSolvent1mg5mg10mgConcentration制备储备液1mM1.7128mL8.5638mL17.1277mL5mM0.3426mL1.7128mL3.4255mL10mM0.1713mL0.8564mL1.7128mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存⽅式和期限：-80°C,6months;-20°C,1month。-80°C储存时，请在6个⽉内使⽤，-20°C储存时，请在1个⽉内使⽤。体内实验请根据您的实验动物和给药⽅式选择适当的溶解⽅案。以下溶解⽅案都请先按照InVitro⽅式配制澄的储备液，再依次添加助溶剂：(为保证实验结果的可靠性，澄的储备液可以根据储存条件，适当保存；体内实验的⼯作液，建议您现⽤现配，当天使⽤；以下溶剂前显⽰的百分⽐指该溶剂在您配制终溶液中的体积占⽐；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的⽅式助溶)1.请依序添加每种溶剂：10%DMSO>>40%PEG300>>5%Tween-80>>45%salineSolubility:1.67mg/mL(2.86mM);Suspendedsolution;Needultrasonic2.请依序添加每种溶剂：10%DMSO>>90%(20%SBE-β-CDinsaline)Solubility:≥1.25mg/mL(2.14mM);Clearsolution1/2MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemE3.请依序添加每种溶剂：10%DMSO>>90%cornoilSolubility:≥1.25mg/mL(2.14mM);ClearsolutionBIOLOGICALACTIVITY⽣物活性HCoV-229E-IN-1HCoV-229E复制的有效抑制剂，在MTS和CPE细胞中的EC50值分别为0.65μM和0.6μM。IC50&TargetIC50:0.65μM(HCoV-229E)[1]体外研究HCoV-229E-IN-1(compound5h)(0.01-100μM)fullysuppressesthecapacityofHCoV-229Ereplicationinadose-dependentmanner[1].HCoV-229E-IN-1(12μM)fullypreventedtheformationofdsRNAintermediatesofCoVRNAsynthesis[1].REFERENCES[1].StevaertA,et,al.BetulonicAcidDerivativesInterferingwithHumanCoronavirus229EReplicationviathensp15Endoribonuclease.JMedChem.2021May13;64(9):5632-5644.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearchuseonly.Tel:400-820-3792;