常用缓冲溶液的配制方法(doc 11)

1、Evaluation Warning: The document was created with Spire.Doc for .NET.常用缓冲溶液的配制（一）甘氨酸-盐酸缓冲液（0.05 mol/L）X毫升0.2 mol/L甘氨酸Y毫升0.2 mol/L HCl，再加水稀释至200毫升。pHXYpHXY2.25044.03.05011.42.45032.43.2508.22.65024.23.4506.42.85016.83.6505.0甘氨酸分子子量775.007。0.2 mmol/L甘氨氨酸溶液液含155.011 g/L。（二）邻苯苯二甲酸酸-盐酸酸缓冲液液（0.05 moll/L）X

2、毫升0.2 mmol/L邻苯苯二甲酸酸氢钾Y毫升0.2 mmol/L HHCl，再再加水稀稀释至220毫升升。pH(200)XYpH (20)XY2.254.67003.251.47002.453.96003.450.99002.653.29552.650.59772.852.64223.850.26333.052.0322邻苯二甲酸酸氢钾分分子量2044.233。0.22 mool/LL邻苯二二甲酸氢氢钾溶液液含400.855 g/L。（三）磷酸酸氢二钠钠-柠檬檬酸缓冲冲液pH0.2 mmol/LNa2HPPO4 (mmL)0.1 mmol/L柠檬酸 (mL)pH0.2 mmol/LNa2H

3、PPO4 (mmL)0.1 moll/L柠檬酸 (mL)2.83.03.84.04.85.00.4074.114.945.706.447.107.718.288.829.359.8610.30019.60018.76617.82216.83315.89915.06614.30013.56612.90012.29911.72211.18810.65510.1449.705.86.06.87.07.88.010.72211.15511.60012.09912.63313

4、.22213.85514.55515.45516.47717.39918.17718.73319.15519.4559.288.858.407.917.376.786.155.454.553.532.611.831.270.850.55Na2HPPO4分子量量1441.998；0.22 mool/LL溶液为为28.40 g/LL。Na2HPPO42H2O分子量量1778.005；0.22 mool/LL溶液为为35.61 g/LL。Na2HPPO412HH2O分子量量3558.222；0.22 mool/LL溶液为为71.64 g/LL。C6H8OO7H2O分子量量2110.114；0.11

5、mool/LL溶液为为21.01 g/LL。（四）柠檬檬酸-氢氢氧化钠钠-盐酸酸缓冲液液pH钠离子浓度度(mol/L)柠檬酸 (g)C6H8OO7H2O氢氧化钠 (g)NaOH 97盐酸 (mmL)HCl (浓)最终体积(L)2.20.2021084160103.10.2021083116103.30.2021083106104.30.202108345105.30.3524514468105.80.45285186105106.50.3826615612610 使用时时可以每每升中加加入1克酚，若若最后ppH值有有变化，再再用少量量50氢氢氧化钠钠溶液或或浓盐酸酸调节，冰冰箱保存存。（五）柠

6、檬檬酸-柠柠檬酸钠钠缓冲液液（0.1 mmol/L）pH0.1 moll/L柠檬酸 (mL)0.1 mmol/L柠檬酸钠 (mLL)pH0.1 mmol/L柠檬酸 (mL)0.1 mmol/L柠檬酸钠 (mLL)3.07.312.73.416.04.05.46.413.63.65.514.53.814.06.05.84.715.34.03.817.24.42.018.04.61.410.8柠檬酸：

7、CC6H8O7H2O分子量量2110.114 ；0.11 mool/LL溶液为为21.01 g/LL。柠檬酸钠：Na3C6H5O72H2O分子量量2994.112 ；0.11 mool/LL溶液为为29.41 g/LL。（六）醋酸酸-醋酸酸钠缓冲冲液（00.2 moll/L）pH(18)0.2 mmol/LNaAc (mmL)0.2 mmol/LHAc (mLL)pH(18)0.2 mmol/LNaAc (mmL)0.2 mmol/LHAc (mLL)3.60.759.354.85.9008.805.07.003.004.01.800

8、57.355.48.601.404.43.706.3004.64.9000.60NaAc3H2O分子量量1336.009；0.22 mool/LL溶液为为27.22 g/LL。冰乙乙酸111.8 mL稀稀释至11 L（需需标定）。（七）磷酸酸二氢钾钾-氢氧氧化钠缓缓冲液（00.055 mool/LL）X 毫升 0.22 mool/LL KHH2PO4+Y毫升升0.22 mool/LL NaaOH 加水稀稀释至220毫升升。pH (20)X (mmL)Y (mmL)pH (20) X (mL) Y (mL)5.850.37227.052.96336.050

9、.57007.253.50006.250.86007.453.95006.451.26007.654.28006.651.78007.854.52006.852.36558.054.6800（八）磷酸酸盐缓冲冲液 磷酸氢氢二钠-磷酸二二氢钠缓缓冲液（0.2 mol/L）pH0.2 mmol/LNa2HPPO4 (mL)0.2 mmol/LNaH2PPO4 (mmL)pH0.2 mmol/LNa2HPPO4 (mL)0.2 mmol/LNaH2PPO4 (mmL)5.88.092.07.061.039.05.910.090.07.167.033.06.012.387.77.272.028.06.

10、115.085.07.377.023.06.218.581.57.481.019.06.322.577.57.584.016.06.426.573.57.687.013.06.531.568.57.789.510.56.637.562.57.843.556.57.993.07.06.849.051.08.055.045.0Na2HPPO42H2O分子量量1778.005；0.22 mool/LL溶液为为35.61 g/LL。Na2HPPO412HH2O分子量量3558.222；0.22 mool/LL溶液为为71.64 g/LL。NaH2PPO4H2O分

11、子量量1338.001；0.22 mool/LL溶液为为27.6 gg/L。NaH2PPO42H2O分子量量1556.003；0.22 mool/LL溶液为为31.21 g/LL。（九）巴比比妥纳-盐酸缓缓冲液pH（18）0.04 moll/L巴比妥钠(mL)0.2 mmol/LHCl (mL)PH（18）0.04 moll/L巴比妥钠(mL)0.2 mmol/LHCl (mL)6.810018.48.41005.217.010017.88.61003.827.210016.78.81002.527.410015.39.01001.657.610013.49.21001.137.810011.

12、4779.41000.708.01009.399.61000.358.21007.21巴比妥钠分分子量2066.188；0.004 mmol/L溶液液为8.25 g/LL。（十）Trris-HCll缓冲液液（0.05 moll/L）50毫升00.1mmol/L三羟羟甲基氨氨基甲烷烷（Trris）溶溶液与XX毫升0.1mool/LL盐酸混混匀并稀稀释至1100毫毫升。pH (25)X (mmL)pH (25)X (mmL)7.1045.78.1026.27.2044.78.2022.97.3043.48.3019.97.4042.08.4017.27.5040.38.5014.77.6038.5

13、8.6012.47.7036.68.7010.37.8034.58.808.57.9032.08.907.08.0029.2Tris分分子量1211.144 ；0.11 mool/LL溶液为为12.1144 g/L。Triis溶液液可从空空气中吸吸收二氧氧化碳，使使用时注注意将瓶瓶盖严。（十一）、硼硼酸-硼硼砂缓冲冲液 (0.22 mool/LL硼酸根根)pH0.05 moll/L硼砂 (mmL)0.2 mmol/L硼酸 (mmL)pH0.05 moll/L硼砂 (mmL)0.2 mmol/L硼酸 (mmL)7.41.09.07.82.08

14、.08.76.04.08.03.07.09.08.02.0硼砂：Naa2B4O710HH2O分子量量3881.443；0.005 mmol/L溶液液（等于于0.22 mool/LL硼酸根根）含119.007 gg/L。硼酸：H33BO3分子量量611.844；0.22 mool/LL的溶液液为122.377 g/L。硼砂易失去去结晶水水，必须须在带塞塞的瓶中中保存。（十二）甘甘氨酸-氢氧化化钠缓冲冲液（00.055 mool/LL）X毫升0.2 mmol/L甘氨氨酸YY毫升0.2 mmol/L NNaOHH加水稀稀释至2200毫毫升。pHX (mmL)Y (mmL)pHX (mmL)Y (mm

15、L)8.6504.09.65022.48.8506.09.85027.29.0508.8105032.09.25012.010.45038.69.45016.810.65045.5甘氨酸分子子量775.007；0.22 mool/LL溶液含含15.01 g/LL（十三）硼硼砂-氢氢氧化纳纳缓冲液液（0.05 moll/L硼硼酸根）X毫升0.05 moll/L硼硼砂YY毫升0.2 mmol/L NNaOHH加水稀稀释至2200毫毫升。pHX (mLL)Y (mLL)pHX (mLL)Y (mLL)9.3506.09.85034.09.45011.010.05043.09.65023.010.15046.0硼砂Na22B4O710HH2O分子量量3881.443；0.005 mmol/L硼砂砂溶液（等等于0.2 mmol/L硼酸酸根）为为19.07 g/LL。（十四）碳碳酸钠-碳酸氢氢钠缓冲冲液（00.1 moll/L）(此缓冲冲液在CCa2、Mgg2存在在时不得得使用).pH0.1