细胞生物学英文课件-Observation of DNA and RNA

1、Observation of DNA and RNA in animal cellsAim of this classLearn how to display cell DNA and RNAObserve the location of cell DNA and RNA2. PrincipleDNA and RNA is acidic，with affinity to basic dye (Methyl green and Pyronin). DNA(high polymerization)+methyl green(+) DNAmethyl green (bluegreen color)

2、RNA(low polymerization)+pyronin(+) RNApyronin (red color)DNA is located in nucleus，so nucleus is dyeing to bluegreen color；RNA is located in cytoplasma, so cytoplasma is dyeing to red color. 3.Operation Making blood smear: from frog blood.Making blood smear Cut off the frog leg, let breakage bleed.D

3、rop the frog blood on one side of the slide.Take another glass slide with smooth edges, to touch the blood drop on the slide. The blood will be along the edge.Make pushing slide and loading slide into 30 to 45angle. Quickly push forward the slide, Make the blood into a thin layer evenly distributedA

4、ttention: Push for a little while to coordination, speed is consistent, uniform appropriate power. How to make blood smear2) Fixation: dip the blood smear in 75% alcohol for 5-10min, dry in the air.3) Dyeing: put blood smear on shelf, dyeing by mixture of methylgreen and pyronin by 20min.4) Flushing: by water5) Differentiation: dip blood smear in pure propanol for seconds.6) Observation: nucleus is dyeing to bluegreen, cytoplasma is dyeing to red. Aim of fixation:Prevent cell from autolysis and decayMaking cells sensitive to dye3. Making tissues to be a little firmerAim of d