Visomitin-SKQ1-DataSheet-生命科学试剂-MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEVisomitinCat. No.: HY-100474CAS No.: 934826-68-3Synonyms: SKQ1分式: CHBrOP分量: 617.6作靶点: Reactive Oxygen Species作通路: Immunology/Inflammation; Metabolic Enzyme/Protease; NF-B储存式: Pure form -20C 3 years4C 2 yearsIn solvent -80C 6 mon

2、ths-20C 1 month溶解性数据体外实验 DMSO : 29 mg/mL (46.96 mM)* means soluble, but saturation unknown.Mass Solvent1 mg 5 mg 10 mg Concentration制备储备液1 mM 1.6192 mL 8.0959 mL 16.1917 mL5 mM 0.3238 mL 1.6192 mL 3.2383 mL10 mM 0.1619 mL 0.8096 mL 1.6192 mL请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液，并请注意储备液的保存式和期限。体内实验 请根据您的实验动物和

3、给药式选择适当的溶解案，配制前请先配制澄清的储备液，再依次添加助溶剂(为保证实验结果的可靠性，体内实验的作液，建议您现现配，当天使；澄清的储备液可以根据储存条件，适当保存；以下溶剂前的百分 指该溶剂在您配制终溶液中的体积占)：1. 请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% salineSolubility: 2.5 mg/mL (4.05 mM); Clear solution2. 请依序添加每种溶剂： 10% DMSO 90% (20% SBE-CD in saline)Solubility: 2.5 mg/mL (4.05 mM); Cl

4、ear solution1/3 Master of Small Molecules 您边的抑制剂师www.MedChemEBIOLOGICAL ACTIVITY物活性 Visomitin种新型抗氧化剂，具有最的线粒体穿透能和有效的抗氧化能。体外研究 Direct treatment of tumor infiltrating leukocytes with Visomitin (SkQ1) does not influence their cytotoxicityagainst Panc02 cells. While Visomitin does not affect viability of

5、 the cell lines, this drug at 500 nMconcentration reduces heavily the proliferation of human PDAC cells 1.体内研究 Regarding systemic angiogenic factors, it is observed in serum of Pancreatic ductal adenocarcinoma (PDAC)bearing mice a decrease in KC in the group of continuous treatment with Visomitin (S

6、kQ1). Treatment of themice with Visomitin increases the level of VEGF molecules. The amount of MIP1a and prolactin is reduced inall Visomitin treatment groups or after the follow-up treatment, respectively. Also, an increase in the IL-6 andIL-13 amount is found in the Visomitin treated groups. TGF-b

7、 amount is decreased in the pretreatmentsetting. On the contrary, all schemes of the Visomitin treatment decrease the NKT cell percentage. TheVisomitin treatment has prolonged the median survival of PDAC-bearing mice, but the difference does notreach the level of significance defined 1.PROTOCOLCell

8、Assay 1 Panc02 cells are treated 48 h with different concentrations of Visomitin (SkQ1). Cell viability after Visomitintreatment is measured with an EZ4U Kit as described by the manufacturers. Briey, 20,000 cells per well areseeded in 96-wellplates and let grow overnight. Afterwards, cells are treat

9、ed without the medium exchange.A substrate compound from the kit is added and the cells are further incubated for 5 hr at 37C to convert theyellow colored tetrazolium to its red formazan derivate by living cells. The absorbance is measured at 450 nm1.MCE has not independently confirmed the accuracy

10、of these methods. They are for reference only.Animal Female C57BL/6 mice are used in this study. For experiments on both acute and chronic pancreatitis, miceAdministration 2 are divided in three groups. Group A (acute pancreatitis (AP) n=8; chronic pancreatitis (CP) n=12) is treatedwith 5nmol/kg Vis

11、omitin (SkQ1), group B (AP n=8; CP n=12) is the untreated control, and group C (AP n=8;CP n=7) is the sham group, which is injected intraperitoneally with 0.9% NaCl instead of cerulein and istherefore the negative control group without pancreatitis. For experiments on acute pancreatitis, mice arepre

12、treated with Visomitin for 8 weeks prior to induction of pancreatitis. Mice designated for experiments onchronic pancreatitis receive Visomitin at the same concentration for 8 weeks in parallel with induction ofpancreatitis 2.MCE has not independently confirmed the accuracy of these methods. They ar

13、e for reference only.户使本产品发表的科研献 Redox Biol. 2018 Oct;19:339-353.See more customer validations on HYPERLINK / www.MedChemE2/3 Master of Small Molecules 您边的抑制剂师www.MedChemEREFERENCES1. Bazhin AV, et al. The novel mitochondria-targeted antioxidant SkQ1 modulates angiogenesis and inflammatory micromilieu in a murineorthotopic model of pancreatic cancer. Int J Cancer. 2016 Jul 1;139(1):130-9.2. Weniger M, et al. The Analgesic Effect of the Mitochondria-Targeted Antioxidant SkQ1 in Pancreatic Inflammation. Oxid Med CellLongev. 2016;2016:465