下载本文档
版权说明:本文档由用户提供并上传,收益归属内容提供方,若内容存在侵权,请进行举报或认领
文档简介
1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemESDMACat. No.: HY-101410CAS No.: 30344-00-4Synonyms: Symmetric dimethylarginine; NG,NG-Dimethyl-L-arginine分式: CHNO分量: 202.25作靶点: Endogenous Metabolite作通路: Metabolic Enzyme/Protease储存式: Powder -20C 3 years4C 2 yearsIn solvent -80C
2、 6 months-20C 1 month溶解性数据体外实验 DMSO : 50 mg/mL (247.22 mM; ultrasonic and adjust pH to 3 with 1M HCl)Mass Solvent1 mg 5 mg 10 mg Concentration制备储备液1 mM 4.9444 mL 24.7219 mL 49.4438 mL5 mM 0.9889 mL 4.9444 mL 9.8888 mL10 mM 0.4944 mL 2.4722 mL 4.9444 mL请根据产品在不同溶剂中的溶解度,选择合适的溶剂配制储备液,并请注意储备液的保存式和期限。体内实验
3、请根据您的实验动物和给药式选择适当的溶解案,配制前请先配制澄清的储备液,再依次添加助溶剂(为保证实验结果的可靠性,体内实验的作液,建议您现现配,当天使;澄清的储备液可以根据储存条件,适当保存;以下溶剂前的百分 指该溶剂在您配制终溶液中的体积占):1. 请依序添加每种溶剂: 10% DMSO 40% PEG300 5% Tween-80 45% salineSolubility: 2.5 mg/mL (12.36 mM); Clear solution2. 请依序添加每种溶剂: 10% DMSO 90% (20% SBE-CD in saline)Solubility: 2.5 mg/mL (1
4、2.36 mM); Clear solution3. 请依序添加每种溶剂: 10% DMSO 90% corn oil1/3 Master of Small Molecules 您边的抑制剂师www.MedChemESolubility: 2.5 mg/mL (12.36 mM); Suspended solution; Need ultrasonicBIOLOGICAL ACTIVITY物活性 SDMA (Symmetric dimethylarginine)氧化氮 (NO) 合酶活性的内源性抑制剂。IC50 & Target Human Endogenous Metabolite体外研究
5、SDMA is the structural isomer of the cardiovascular risk marker asymmetric dimethylarginine, as anendogenous marker of renal function. SDMA does not directly inhibit NOS but is a competitor of argininetransport. SDMA is primarily eliminated by renal excretion and is a promising endogenous marker ofg
6、lomerular filtration rate 1. SDMA inhibits dose dependently the NO synthesis in intact endothelial cells,whereas it has no effect on protein expression of NOS 1. SDMA is involved in the inflammatory process ofchronic kidney disease, activating NF-B and resulting in enhanced expression of IL-6 and TN
7、F- 2.体内研究 SDMA is highly stable in serum and plasma, and the assay demonstrates excellent analytical performance.In unaffected dogs, SDMA remains unchanged whereas in affected dogs, SDMA increases during diseaseprogression, correlating strongly with an increase in sCr and decrease in GFR 3. Chronic
8、SDMA infusionleads to a significant increase of SDMA levels in mice, but the GFR did not change at 4 weeks. Nohistological changes are observed, particularly no effect on fibrosis or endothelias nitric oxide synthaseexpression. There is neither an effect of SDMA on systolic blood pressure nor on eje
9、ction fraction 4.PROTOCOLCell Assay 2 SDMA stock solution is prepared in 0.9% NaCl and diluted in the cell culture medium or in heparinized wholeblood resulting in a maximal uremic concentration of 6.1 M SDMA. Whole blood is incubated with saline(control) or different doses of ADMA (0.6, 3.6, and 36
10、 M) or SDMA (1.5, 3.1, and 6.1 M) for 2 hours in ahumidified atmosphere of 5% CO2 in air at 37C. Cells are finally stained for intracellular TNF- or IL-6.Samples are analyzed with a flow cytometer 2.MCE has not independently confirmed the accuracy of these methods. They are for reference only.Animal
11、 Mice: Eight-week-old male C57Bl/6 mice receives vehicle-controlled infusion of SDMA (250 mol/kg/days) forAdministration 4 28 days using osmotic minipumps (n=24/group). Glomerular filtration rate, cardiac function and bloodpressure are monitored. Blood samples for SDMA determination are obtained at
12、baseline, 2 and 4 weeks.Mice are euthanized at 4 weeks to obtain tissue for renal histology 4.MCE has not independently confirmed the accuracy of these methods. They are for reference only.REFERENCES1. Bode-B?ger SM,et al. Symmetrical dimethylarginine: a new combined parameter for renal function and
13、 extent ofcoronary artery disease.2. Schepers E, et al. Symmetric dimethylarginine as a proinflammatory agent in chronic kidney disease.Clin J Am Soc Nephrol. 2011Oct;6(10):2374-83.2/3 Master of Small Molecules 您边的抑制剂师www.MedChemE3. Nabity MB, et al. Symmetric Dimethylarginine Assay Validation, Stab
14、ility, and Evaluation as a Marker for the EarlyDetection of ChronicKidney Disease in Dogs. J Vet Intern Med. 2015 Jul-Aug;29(4):1036-44.4. Veldink H, et al. Effects of chronic SDMA infusion on glomerular filtration rate, blood pressure, myocardial function and renal histology inC57BL6/J mice. Nephrol Dial Transplant. 2013 Jun;28(
温馨提示
- 1. 本站所有资源如无特殊说明,都需要本地电脑安装OFFICE2007和PDF阅读器。图纸软件为CAD,CAXA,PROE,UG,SolidWorks等.压缩文件请下载最新的WinRAR软件解压。
- 2. 本站的文档不包含任何第三方提供的附件图纸等,如果需要附件,请联系上传者。文件的所有权益归上传用户所有。
- 3. 本站RAR压缩包中若带图纸,网页内容里面会有图纸预览,若没有图纸预览就没有图纸。
- 4. 未经权益所有人同意不得将文件中的内容挪作商业或盈利用途。
- 5. 人人文库网仅提供信息存储空间,仅对用户上传内容的表现方式做保护处理,对用户上传分享的文档内容本身不做任何修改或编辑,并不能对任何下载内容负责。
- 6. 下载文件中如有侵权或不适当内容,请与我们联系,我们立即纠正。
- 7. 本站不保证下载资源的准确性、安全性和完整性, 同时也不承担用户因使用这些下载资源对自己和他人造成任何形式的伤害或损失。
最新文档
- 《固体废物处理与处置》大学笔记
- 2023高考英语语法填空热点话题分类训练:个人情况
- 济南2024年10版小学英语第5单元测验卷
- 2025新译林版英语七年级下Unit 8 Wonderland单词表
- 强社会救助体系建设的调研
- 消防突发状况的应急预案(3篇)
- 运输合同(水陆联运)(35篇)
- 试用期转正总结(33篇)
- 致客户慰问信
- 集团2024年工作计划6篇
- 《这儿真美》优秀课件
- DB15T 1276-2017 公路波纹钢管(板)桥涵工程质量检验评定标准
- hypertension高血压全英语版-课件
- 高中政治课程标准解读 汇报课件
- 心身疾病-课件
- 物理名词解释
- 主要股东或出资人信息及投标人基本情况表模板
- 养老院 入住申请表
- 【审计工作底稿模板】SA营业收入
- 2022年《学习有方法教案》初中心理健康教育鲁画报社版六年级全一册教案
- 怡安翰威特:高潜人才标准构建技术与案例分享课件
评论
0/150
提交评论