Diphenylterazine-DTZ-DataSheet-MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEDiphenylterazineCat. No.: HY-111382CAS No.: 344940-63-2Synonyms: DTZ分式: CHNO分量: 377.44作靶点: Others作通路: Others储存式: 4C, protect from light, stored under nitrogen* In solvent : -80C, 6 months; -20C, 1 month (protect fromlight, store

2、d under nitrogen)溶解性数据体外实验 DMSO : 19 mg/mL (50.34 mM)H2O : 40% PEG300 5% Tween-80 45% salineSolubility: 2 mg/mL (5.30 mM); Clear solution2. 请依序添加每种溶剂： 10% DMSO 90% corn oilSolubility: 2 mg/mL (5.30 mM); Clear solution1/2 Master of Small Molecules 您边的抑制剂师www.MedChemEBIOLOGICAL ACTIVITY物活性 Diphenylter

3、azine种物发光剂。体外研究 Diphenylterazine is a bioluminescence agent. Diphenylterazine alone yields very little background, leading toexcellent signal-to-background ratios. Furthermore, Diphenylterazine elicits minimal cell toxicity at millimolarconcentrations. Diphenylterazine injections into untransfected

4、BALB/c mice do not yield any backgroundemission. The bioluminescence resulting from intraperitoneally injected Diphenylterazine displays extendedkinetics 1.PROTOCOLCell Assay 1 BALB/c mice are used and transfected with cells expressing teLuc, Antares, Antares2 and FLuc by injectingcells into the tai

5、l vein of BALB/c mice. After the diminishing of the FLuc bioluminescence, 0.3 molDiphenylterazine or furimazine is intraperitoneally injected. Mice are imaged with a 1-min exposure per frameover a course of 20 min. The images are processed using the Fiji image analysis software and the frameswith hi

6、ghest signals in individual experiments are used for comparison 1.MCE has not independently confirmed the accuracy of these methods. They are for reference only.REFERENCES1. Yeh HW, et al. Red-shifted luciferase-luciferin pairs for enhanced bioluminescence imaging. Nat Methods. 2017 Oct;14(10):971-974.McePdfHeightCaution: Product has not been fully validated for medical applications.For research use only.Tel: 40