血管紧张素Ⅱ作用机制 - Medchemexpress - MCE中国

1、Product Data SheetAngiotensin II humanCat. No.: HY-13948CAS No.: 4474-91-3分式: CHNO分量: 1046.18Sequence: Asp-Arg-Val-Tyr-Ile-His-Pro-PheSequence Shortening: DRVYIHPF作靶点: Angiotensin Receptor; Apoptosis作通路: GPCR/G Protein; Apoptosis储存式: Protect from light, stored under nitrogenPowder -80C 2 years-20C 1

2、 yearIn solvent -80C 6 months-20C 1 month溶解性数据体外实验 H2O : 50 mg/mL (47.79 mM)DMSO : 16.67 mg/mL (15.93 mM; Need ultrasonic)* means soluble, but saturation unknown.SolventMass1 mg 5 mg 10 mgConcentration制备储备液1 mM 0.9559 mL 4.7793 mL 9.5586 mL5 mM 0.1912 mL 0.9559 mL 1.9117 mL10 mM 0.0956 mL 0.4779 mL

3、0.9559 mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存式和期限：-80C, 6 months; -20C, 1 month (protect from light, stored under nitrogen)。-80C 储存时，请在 6 个内使，-20C 储存时，请在 1 个内使。体内实验请根据您的实验动物和给药式选择适当的溶解案。以下溶解案都请先按照 In Vitro 式配制澄清的储备液，再依次添加助溶剂：为保证实验结果的可靠性，澄 的储备液可以根据储存条件，适当保存；体内实验的作液，建议您现现配，当天使；

4、 以下溶剂前显的百分 指该溶剂在您配制终溶液中的体积占；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的式助溶1. 请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% salineSolubility: 0.83 mg/mL (0.79 mM); Clear solution此案可获得 0.83 mg/mL (0.79 mM，饱和度未知) 的澄清溶液。Page 1 of 2 www.MedChemE以 1 mL 作液为例，取 100 L 8.3 mg/mL 的澄 DMSO 储备液加到 400 L PEG300 中，混合均匀；向上述体系中加50

5、 L Tween-80，混合均匀；然后继续加 450 L 理盐定容 1 mL。2. 请依序添加每种溶剂： 10% DMSO 90% (20% SBE-CD in saline)Solubility: 0.83 mg/mL (0.79 mM); Clear solution此案可获得 0.83 mg/mL (0.79 mM，饱和度未知) 的澄清溶液。以 1 mL 作液为例，取 100 L 8.3 mg/mL 的澄 DMSO 储备液加到 900 L 20% 的 SBE-CD 理盐溶液中，混合均匀。3. 请依序添加每种溶剂： 10% DMSO 90% corn oilSolubility: 0.83

6、 mg/mL (0.79 mM); Clear solution此案可获得 0.83 mg/mL (0.79 mM，饱和度未知) 的澄 溶液，此案不适于实验周 期在半个以上的实验。以 1 mL 作液为例，取 100 L 8.3 mg/mL 的澄 DMSO 储备液加到 900 L 油中，混合均匀。BIOLOGICAL ACTIVITY物活性 Angiotensin II human (Angiotensin II) 主 作于 AT1 受体的管收缩剂。Angiotensin II human 刺激交感神经刺激 ，增加醛 酮的物合成和肾功能。Angiotensin II human 诱导管平滑肌细胞

7、的长，增加成纤维细胞中 I 型和 III 型胶原的合成，导致管壁和肌增厚以及纤维化。Angiotensin II human 还可诱导细胞凋亡 (apoptosis)。IC & Target Angiotensin receptor (AT receptor)1体外研究 Most of the known actions of Angiotensin II (Ang II) are mediated by AT1 receptors, the AT2 receptor contributes tothe regulation of blood pressure and renal functi

8、on1. Angiotensin II raises blood pressure (BP) by a number ofactions, the most important ones being vasoconstriction, sympathetic nervous stimulation, increased aldosteronebiosynthesis and renal actions. Other Angiotensin II actions include induction of growth, cell migration, and mitosis ofvascular

9、 smooth muscle cells, increased synthesis of collagen type I and III in fibroblasts, leading to thickening of thevascular wall and myocardium, and fibrosis. These actions are mediated by type 1 Ang II receptors (AT1)2. At thecellular level, responsiveness to Angiotensin II is conferred by the expres

10、sion of the two classes of angiotensinreceptors (AT1 and AT2). The effects of Angiotensin II to increase blood pressure are mediated by AT1 receptors3.体内研究 To distinguish the AT1 receptor population that is critical for the pathogenesis of hypertension, osmotic minipumps are implanted s.c. into each

11、 animal to infuse Angiotensin II (1,000 ng/kg/min) continuously for 4 weeks. Angiotensin II causes hypertension by activating AT1 receptors in the kidney promoting sodium reabsorption3.PROTOCOLAnimal Mice3Administration 3 (129C57BL/6) F1 mice lacking AT1A receptors for Angiotensin II are used. The m

12、ice are fed 10 gm/day gelled 0.25%NaCl diet that contains all nutrients and water. After 1 week of baseline collections, the animals are implanted withosmotic minipumps infusing Angiotensin II and are returned to the metabolic cage for 5 more days. Urinary sodiumcontent is determined by using an IL9

13、43 Automatic Flame photometer. After 28 days of Angiotensin II infusion,hearts are harvested, weighed, fixed in formalin, sectioned, and stained with Masson trichrome. All of the tissues areexamined by a pathologist (P.R.) without knowledge of genotypes.MCE has not independently confirmed the accura

14、cy of these methods. They are for reference only.Page 2 of 3 www.MedChemE户使本产品发表的科研献 Oxid Med Cell Longev. 2019; 2019: 4025496. Oxid Med Cell Longev. 2019 Nov. J Cell Mol Med. 2020 Mar 12. J Cell Mol Med. 2019 Sep 11. Am J Physiol Cell Physiol. 2020 Mar 11.See more customer validations on HYPERLINK

15、www.MedChemE www.MedChemEREFERENCES1. de Gasparo M, et al. International union of pharmacology. XXIII. The angiotensin II receptors. Pharmacol Rev. 2000 Sep;52(3):415-72.2. Fyhrquist F, et al. Role of angiotensin II in blood pressure regulation and in the pathophysiology of cardiovascular disorders. J Hum Hypertens. 1995Nov;9 Suppl 5:S19-24.3. Crowley SD, et al. Angiotensin II causes hypertension and cardiac hypertrophy through its receptors in the kidney. Proc Natl Acad Sci U S A. 2006 Nov21;103