果糖作用机制 - Medchemexpress - MCE中国

1、Product Data SheetFructoseCat. No.: HY-N0395CAS No.: 7660-25-5分式: CHO分量: 180.16作靶点: Others作通路: Others储存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性数据体外实验 H2O : 100 mg/mL (555.06 mM; Need ultrasonic)DMSO : 100 mg/mL (555.06 mM)* means soluble, but saturation unknown.Solvent

2、Mass1 mg 5 mg 10 mgConcentration制备储备液1 mM 5.5506 mL 27.7531 mL 55.5062 mL5 mM 1.1101 mL 5.5506 mL 11.1012 mL10 mM 0.5551 mL 2.7753 mL 5.5506 mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存式和期限：-80C, 6 months; -20C, 1 month。-80C 储存时，请在 6 个内使，-20C 储存时，请在 1 个内使。体内实验请根据您的实验动物和给药式选择适当的溶解案。

3、以下溶解案都请先按照 In Vitro 式配制澄清的储备液，再依次添加助溶剂：为保证实验结果的可靠性，澄 的储备液可以根据储存条件，适当保存；体内实验的作液，建议您现现配，当天使； 以下溶剂前显的百分 指该溶剂在您配制终溶液中的体积占；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的式助溶1. 请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% salineSolubility: 2.5 mg/mL (13.88 mM); Clear solution此案可获得 2.5 mg/mL (13.88 mM，饱和度未知) 的澄清溶液。以 1 mL

4、作液为例，取 100 L 25.0 mg/mL 的澄 DMSO 储备液加到 400 L PEG300 中，混合均匀；向上述体系中加50 L Tween-80，混合均匀；然后继续加 450 L 理盐定容 1 mL。2. 请依序添加每种溶剂： 10% DMSO 90% (20% SBE-CD in saline)Solubility: 2.5 mg/mL (13.88 mM); Clear solutionPage 1 of 2 www.MedChemE此案可获得 2.5 mg/mL (13.88 mM，饱和度未知) 的澄清溶液。以 1 mL 作液为例，取 100 L 25.0 mg/mL 的澄

5、DMSO 储备液加到 900 L 20% 的 SBE-CD 理盐溶液中，混合均匀。3. 请依序添加每种溶剂： 10% DMSO 90% corn oilSolubility: 2.5 mg/mL (13.88 mM); Clear solution此案可获得 2.5 mg/mL (13.88 mM，饱和度未知) 的澄 溶液，此案不适于实验周 期在半个以上的实验。以 1 mL 作液为例，取 100 L 25.0 mg/mL 的澄 DMSO 储备液加到 900 L 油中，混合均匀。BIOLOGICAL ACTIVITY物活性 Fructose在许多植物中发现的简单的酮类单糖，它常常与葡萄糖结合形成

6、糖蔗糖。体外研究 Fructose, at low concentrations do not cause any significant increase of Tissue factor (TF)-mRNA levels. On thecontrary, higher Fructose concentrations cause increase in TF mRNA levels at 60 min, as compare to unstimulatedcells. Increasing Fructose concentrations causes significant decrease

7、 of tPA-mRNA levels. SOD significantly preventsFructose induced NF-B activation which is associated with the parallel reduction of Fructose-induced TFexpression/activity1.体内研究 In mice fed 0% Fructose, portal (0.0600.006 mM, overall mean for all time points) and systemic (0.0300.003 mM)Fructose conce

8、ntrations do not vary with time after feeding. In contrast, portal concentrations in wild-type mice consuming 20% Fructose increase by more than twofold from time (t)=0 to t=1 h after feeding (0.13 mM). Likewise,systemic serum Fructose goes from 0.037 at t=0 to 0.13 mM 1 h after feeding. Fasted (t=0

9、) serum Fructose in the 20%group is similar to postprandial concentrations in the 0% mice for both portal and systemic levels, suggesting that thebaseline Fructose concentration during fasting is not affected by diet. Serum Fructose concentrations in KHK-/- miceare 5- to 100-fold greater than those

10、in wild-type mice for the same diet, time, and sample location. Mean (for alltime points) portal and systemic glucose concentrations in mice fed 20% Fructose are 3 (P=0.004) and 2 (P=0.04)mM greater, respectively, than those in mice fed 0%. Systemic Fructose concentrations are approximately threefol

11、dgreater in KHK-/- mice fed Fructose compare with those fed glucose, but are similar between glucose- and Fructose-fed wild-type mice2.PROTOCOLCell Assay 1 HUVECs are incubated with Fructose (0.25, 1 and 2.5 mM) for 30 min. Then, cells are washed with PBS and then freshmedium is added. Total mRNA is

12、 extracted by cell cultures using TRIzol reagent, at baseline and 60 min after Fructosestimulation and Tissue factor (TF) mRNA levels are examined by realtime reverse transcription (RT) and polymerasechain reaction (PCR). In positive control experiments, HUVECs are incubated with LPS (50 g/mL), for

13、30 min and thenmRNA is extracted at 60 min1.MCE has not independently confirmed the accuracy of these methods. They are for reference only.Animal 50 young adult (7-wk-old) male C57BL6 wild-type mice (18 g) are divided into 10 cages and acclimatized to aAdministration 2 reversed light cycle. Mice are

14、 fed a nonpurified commercial diet ad libitum for the first 4 days. On the 5th day andthen throughout the experiment, diets are removed at 2001 (lights on) and returned at 0801 (lights off). For days 8 to14, diets are switched to pellets containing either 0% Fructose, 10% sucrose, 20% glucose (terme

15、d as 0% Fructose)or 20% Fructose, 10% sucrose, or 0% glucose (20% Fructose). On the 15th day, mice are killed at 0800 before feedingand 0900, 1030, 1200, and 1530 during the dark phase, with n=5 for each time point and diet2.MCE has not independently confirmed the accuracy of these methods. They are

16、 for reference only.Page 2 of 3 www.MedChemEREFERENCES1. Cirillo P, et al. Fructose induces prothrombotic phenotype in human endothelial cells : A new role for added sugar in cardio-metabolic risk. J ThrombThrombolysis. 2015 Nov;40(4):444-51.2. Patel C, et al. Effect of dietary fructose on portal and systemic serum fructose levels in rats and in KHK-/- and GLUT5-/- mice. Am J Physiol GastrointestLiver Physiol. 2