讲义相关周汇报

1、GroupDiscussionPart 1 Mapping of the QTL conferring resistance to leaf and panicle blast in Japonica landrace JiangnanwanIsolatesOriginJNWSYN leaf blastpanicle blast leaf blastpanicle blast191(ZB13)JSHRMRHSHS97-2(ZC15)JSHRMRHSHS35-1(ZD1)JSHRHRHSHS55-1(ZE3)JSHRMRHSHS42-2(ZF3)JSSMSHSHS113(ZG1)JSRSHSHS

2、Hoku 1(ZG1)JapanHRMRHSHSTable 1 the resistance of parents after infect the M.oryzae isolatesSYNJNWSYNJNWAB图1 A 江南晚和苏御糯稻瘟病抗感表型；B 接种北1后的病级类型Fig.1 A Phenotypes of panicle and leaf blast in JNW (left) and SYN(right). B The disease scores after infected hoku1Diseased grains(%)Score of leaf blast severity

3、* P=0.01* P=0.05*SYNJNWSYNJNW2040608012345Results图 2 （A-C) 苏御糯与江南晚杂交不同后代中苗瘟抗性级别分布Figure 2 (A-C) Frenquency distribution of leaf blast among different progeny lines derived from a cross between JNW and SYN. ACBNumber of linesScore of leaf blast severityScore of leaf blast severityScore of leaf blast

4、severityNumber of linesNumber of linesF2:6 NJAU F2:6 JAASF2:7 JAASResults图 3 苏御糯与江南晚杂交不同后代中苗瘟抗性级别分布Fig.3 Frenquency distribution of panicle blast among different progeny lines derived from a cross between JNW and SYNResultsTraitQTLChrPositionLeftMarker RightMarkerLOD PVE(%)AddLBRq.lb 4.1(2014JS)4143

5、RM6089RM38143.67485.61520.3095q.lb 11.1(2014JS)11217RM27273BS3314.430524.57870.6499q.lb 11.3(2015JS)11217RM27273BS3320.811237.19140.9699q.lb 11.3(2015JS)11217RM27273BS336.244212.27140.2034PBRq.pb 7.1(2014JS)778RM3186RM3464.86774.8850-0.0714q.pb 11.1(2014JS)11237BS39BS3329.994148.22330.2261q.pb 4.1(2

6、015HN)4110RM6089RM38144.03183.27840.0577q.pb 5.1(2015HN)59RM1182RM2332.84464.37050.0663q.pb 11.2(2015HN)11207RM7277RM271223.94393.0996-0.0556q.pb 11.3(2015HN)11235RM27273BS3949.199870.18320.2678q.pb 4.1(2015JS)40RM16335RM82133.61743.70950.0627q.pb11.4(2015JS)11228RM27150RM272736.14237.05110.0865q.pb

7、11.5(2015JS)11237BS39BS3322.590831.87900.1884Analyzed by QTL IciMapping V4.0Table 2 All QTLs detected for disease gradeResultsFig.4 (A-C) the correlations between panicle and leaf blast resistance severity；X: The lesion scores panicle blast， Y: The lesion scores leaf blast CABResultsRM7277RM27122RM2

8、7273RM27129BS39RM27150RM27381Chr111.5cM1.006 5.9535.973 8.341 7-4-7R6430-1-3R1-5-611-1-10SSQpb1(250kb）?100kbRM27150RM27381BS33JiangnanwanSuyunuoJ/S927100520.5147.5(2)(0)(0)(2)(2)Figure4 A Genetic and physical maps of the region of the qpb1 locus.RM27273RM27381BC2F1+BC1F1260株结合带型挑选单株接种W28W26BS71(1)(0

9、)(0)150132143Results精细定位Pb-jnw1等待接种表型结果。验证Pb-jnw1是否同时影响苗瘟抗性挑选携带Pb-jnw1纯合位点的单株苗期接种，观察抗感情况。构建育种中间材料将江南晚与南粳46、南粳9108及镇稻88杂交得到的F1，结合分子标记挑选携带Pb-jnw1位点的后代个体分别南粳46、南粳9108、镇稻88回交构建育种中间材料。讨论穗瘟接种及统计发病方法的优化家系不同的生育期一定要设置多个抗感对照；百分比统计方法的依据？抗感的区分？为什么出现偏分布情况？BPart 2 Graduation Dissertation第一章 文献综述1 数量性状的研究1.1 数量性状基因座的定位1.1.1 连锁定位1.1.2 关联定位1.2 QTL克隆2 水稻抗稻瘟病性的研究进展 2.1 水稻稻瘟病的侵染途径和发病类型2.2 水稻稻瘟病发病影响因素和接种鉴定2.3 水稻抗稻瘟病类型和遗传研究2.4 水稻抗稻瘟病基因的定位2.5 水稻抗稻瘟病基因的克隆2.6 水稻抗稻瘟病基因的类型2.7 抗稻瘟病育种应用第二章 水稻