盐酸甲基苄肼作用机制 - Medchemexpress - MCE中国

1、Product Data SheetProcarbazine HydrochlorideCat. No.: HY-13733CAS No.: 366-70-1分式: CHClNO分量: 257.76作靶点: DNA Alkylator/Crosslinker作通路: Cell Cycle/DNA Damage储存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性数据体外实验 DMSO : 16.67 mg/mL (64.67 mM; Need ultrasonic)SolventMass1 mg 5 m

2、g 10 mgConcentration制备储备液1 mM 3.8796 mL 19.3979 mL 38.7958 mL5 mM 0.7759 mL 3.8796 mL 7.7592 mL10 mM 0.3880 mL 1.9398 mL 3.8796 mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存式和期限：-80C, 6 months; -20C, 1 month。-80C 储存时，请在 6 个内使，-20C 储存时，请在 1 个内使。体内实验请根据您的实验动物和给药式选择适当的溶解案。以下溶解案都请先按照 In

3、 Vitro 式配制澄清的储备液，再依次添加助溶剂：为保证实验结果的可靠性，澄 的储备液可以根据储存条件，适当保存；体内实验的作液，建议您现现配，当天使； 以下溶剂前显的百分 指该溶剂在您配制终溶液中的体积占；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的式助溶1. 请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% salineSolubility: 1.67 mg/mL (6.48 mM); Clear solution此案可获得 1.67 mg/mL (6.48 mM，饱和度未知) 的澄清溶液。以 1 mL 作液为例，取 100 L

4、16.699999 mg/mL 的澄清DMSO 储备液加到 400 L PEG300 中，混合均匀；向上述体系中加 50 L Tween-80，混合均匀；然后继续加 450 L 理盐定容 1 mL。2. 请依序添加每种溶剂： 10% DMSO 90% (20% SBE-CD in saline)Solubility: 1.67 mg/mL (6.48 mM); Clear solution此案可获得 1.67 mg/mL (6.48 mM，饱和度未知) 的澄清溶液。Page 1 of 2 www.MedChemE以 1 mL 作液为例，取 100 L 16.699999 mg/mL 的澄，混合

5、均匀。DMSO 储备液加到 900 L 20% 的 SBE-CD 理盐溶液中3. 请依序添加每种溶剂： 10% DMSO 90% corn oilSolubility: 1.67 mg/mL (6.48 mM); Clear solution此案可获得 1.67 mg/mL (6.48 mM，饱和度未知) 的澄 溶液，此案不适于实验周 期在半个以上的实验。以 1 mL 作液为例，取 100 L 16.699999 mg/mL 的澄 DMSO 储备液加到 900 L 油中，混合均匀。BIOLOGICAL ACTIVITY物活性 Procarbazine Hydrochloride种烷化剂 (al

6、kylator)，具有抗肿瘤的作。体外研究 Procarbazine Hydrochloride is an anticancer agent. Procarbazine is not cytotoxic to the LI 210 cells which lackcytochrome P-450 or monoamine oxidase activity1.体内研究 Procarbazine Hydrochloride (50 mg/kg, i.p.) causes micronuclei in hematopoietic cells, but does not increase the l

7、acZmutant frequency (MF) in bone marrow of mice, similar to that in liver, testis, spleen, kidney, and lung. ProcarbazineHydrochloride (50 mg/kg, i.p.) has positive effect on lung, bone marrow, and spleen for carcinogenesis2.Procarbazine (450 mg/kg) significantly decreases testicular and epididymal

8、weight and drastically reduces haploidcells and spermatogenic arrest in hamster3.PROTOCOLCell Assay 1 Following Procarbazine or metabolite treatment, cells are diluted to 50.000/mL in 25-cm2 culture flasks (10 mL).Every 24 h, a 0.5-mL aliquot is removed, diluted 20-fold in Hematall isotonic diluent,

9、 and the cell number determinedwith a Coulter Model F electronic cell counter. Counts greater than 10,000/0.5 mL are corrected for coincidence. Cellsare diluted in fresh culture media when cell density exceeded 1 106/mL. Cultures are maintained until theaggregate cell number approached 100 106/mL an

10、d doubling time has returned to 12 h. Cell survival is determinedusing Equation A, where TD(doubling time for cells of interest) is 12 h1.MCE has not independently confirmed the accuracy of these methods. They are for reference only.Animal Mice2Administration 2 Male MutaTM Mouse animals (7-8 weeks o

11、ld) are used after 2 weeks of acclimation. In the first experiment, 18 miceare injected intraperitoneally (i.p.) with 50 mg/kg Procarbazine hydrochloride in 10 mL saline/kg and eight mice areinjected with 10 mL saline/kg as the vehicle control. Six treated mice are killed 7, 14, and 28 days after tr

12、eatment,and four control mice are killed 7 and 28 days after the treatment. Killing is by cervical dislocation2.MCE has not independently confirmed the accuracy of these methods. They are for reference only.户使本产品发表的科研献 J Mol Med (Berl). 2019 Aug;97(8):1183-1193. Mol Imaging Biol. 2019 Apr 15.See mor

13、e customer validations on HYPERLINK www.MedChemE www.MedChemEPage 2 of 3 www.MedChemEREFERENCES1. Erikson JM, et al. Cytotoxicity and DNA damage caused by the azoxy metabolites of procarbazine in L1210 tumor cells. Cancer Res. 1989 Jan 1;49(1):127-33.2. Suzuki T, et al. Procarbazine genotoxicity in the MutaMouse; strong clastogenicity and organ-specific induction of lacZ mutations. Mutat Res. 1999 Aug18;444(2):269-81.3. Weissenberg R, et al. Procarbazine effects on spermatogenesis in golden hamster: a flow cytometric evaluation. Arch Androl. 2002 Mar-Apr;48(2):91-100.McePdfHeightC