生理生化实验室简介

1、生理生化實驗室簡介主持人：莊秀琪 (獸醫系館 308)一、學歷：營養免疫學博士 Auburn University, USA動物生理學碩士 Auburn University, USA 博士後研究 生物醫學工程研究所 University of California, San Diego, USA二、連絡電話：08-7740370 Email: .tw 三、教授課程：獸醫生理學、獸醫生理學實習、細胞分子免疫學、免疫學、生物化學、分子生物學四、實驗室成員：(包含助理、研究生、大學部學生)助理：曾莉卿博士班研究生：洪麗香碩士班研究生：謝宇翔、張佳蓉、許慧

2、君、李欣佩、李尚玲、蔡嘉慧、蔡佩君五、實驗室目標或發展特色：1. 中草藥成份對中樞神經系統抗焦慮功能。2. 腎功能疾病與糖尿病腎功能異常之細胞激素基因多樣性探討。3. 探討DNA佐劑及其他菌體成份對免疫細胞之作用機轉，以期突破疫苗開發技術。4. 開發細胞激素佐劑。六、研究成果：A.專利1. 大量取得豬隻骨髓造血母細胞以製備試管內分化為樹狀突細胞的方法(發明第I272307號)2. The Anti-hypersensitive Inflammation and Anti-allergicActivities of Zingiber zerumbet (L.) Smith(發明第I282281號

3、)3. 可穩定表現豬顆粒球巨噬細胞群落刺激因子之中國倉鼠卵巢細胞株 (中華民國專利申請中)4. 禽用疫苗之CpG DNA佐劑 (中華民國專利申請中)5. CpG DNA ADJUVANT IN AVIAN VACCINES (美國專利申請中)6. 含有可穩定表現家禽介白素18之基因的中國倉鼠卵巢細胞株 (中華民國專利申請中)7. 生物活性微脂粒及其製造方法 (中華民國專利申請中)8. A COMPETITIVE RECEPTOR BINDING ASSAY FOR DETECTING BETA-GLUCANS HAVING IMMUNOMODULATORY ACTIVITY TO HUMAN

4、CELLS (中華民國專利申請中)9. A COMPETITIVE RECEPTOR BINDING ASSAY FOR DETECTING BETA-GLUCANS HAVING IMMUNOMODULATORY ACTIVITY TO HUMAN CELLS (美國專利申請中)10. 受體融合蛋白基因之表面表現質體、轉型株及其用於活體定量可溶性-葡聚醣之方法 (中華民國專利申請中)B.技術移轉1. 以家禽免疫細胞檢測免疫調節活性成分之篩選技術2. 多醣體免疫調節活性之細胞篩選技術3. 大量取得豬隻骨髓造血母細胞以製備試管內分化為樹狀突細胞的方法4. 豬芽胞梭菌類毒素疫苗5. 豬隻生殖與呼吸

5、綜合症病毒次單位疫苗6. 豬隻第二型環狀病毒次單位疫苗C. 期刊論文：1. Chaung HC*, Chen CW, Hsieh BL, Chung WB*. Toll-like receptor expressions in porcine alveolar macrophages and dendritic cells in responding to poly IC stimulation and porcine reproductive and respiratory syndrome virus (PRRSV) infection. Comparative Immunology, M

6、icrobiology & Infectious Diseases 2009. (accepted)（SCI） 2. Liu CH, Chaung HC*, Chang HL, Peng YT, Chung WB*. Expression of Toll-like receptor mRNA and cytokines in pigs infected with porcine reproductive and respiratory syndrome virus. Veterinary Microbiology 2009. (accepted) （SCI）3. Peng YT, Ch

7、aung HC*, Chang HL, Chang HC, Chung WB*. Modulations of phenotype and cytokine expression of porcine bone marrow-derived dendritic cells by porcine reproductive and respiratory syndrome virus. Veterinary Microbiology 2009. (accepted) (short communication)（SCI）4. 吳家彰、莊秀琪、彭郁棠、張清華、鍾文彬。2009。豬樹狀突細胞受豬假性狂犬

8、病病毒感染後其表現型及功能的改變。台灣獸醫誌5. Chaung HC*, Lien Y, Chung WB. Leukotriene C4 release and gene expressions of IL-8 and MCP-1 in porcine alveolar epithelial type II cells. Res Vet Sci 2009;86:78-87. （SCI）6. Chaung HC*, Hsia LC, Liu SH. The effects of vitamin A supplementation on the production of hypersensit

9、ive inflammatory mediators of ammonia-induced airway in pigs. Food Agr Immun 2008;19(4):283-291.（SCI） 7. Chaung HC*, Ho CT, Huang TC. Anti-hypersensitive and anti-inflammatory activities of water extract of Zingiber zerumbet (L.) Smith. Food Agr Immun 2008;19(2):117-129.（SCI）8. Chang HC, Peng YT, Ch

10、ang HL, Chaung HC*, Chung WB*. Phenotypic and functional modulation of bone marrow-derived dendritic cells by porcine reproductive and respiratory syndrome virus. Veterinary Microbiology 2008; 129(3-4):281-293.（SCI）(3)9. Lin CC, Chen SJ*, Huang KL, Lee WJ, Lin WY, Tsai JH, Chaung HC*. PAHs, PAH-indu

11、ced carcinogenic potency, and particle-extract-induced cytotoxicity of traffic-related nano/ultrafine particles. Environmental Science & Technology 2008;42(11): 4229-4235.（SCI）10. Hsueh-Tao Liu, Hso-Chi Chaung, Hsiu-luan Chang, Ching-Ping Tsai, En-Chung Lin, Ping-Cheng Yang, Wen-Bin Chung. 2008.

12、 Decay of Maternally Derived Antibodies and Seroconversion to Respiratory Viral Infection in Pig Herds. Taiwan Vet J 34:127-141.11. Chen SC, Chen YC, Kwang J, Manopo I, Wang PC, Chaung HC, Liaw LL, Chiu SH. Metschnikowia bicuspidata dominates in Taiwanese cold-weather yeast infections of Macrobrachi

13、um rosenbergii. Dis Aquat Organ. 2007 May 9;75(3):191-9.（SCI）12. Lin CC, Chen SJ, Huang KL, Lee WJ, Lin WY, Liao CJ, Chaung HC, Chiu CH*. 2007. Water-soluble ions in nano/ultrafine/fine/coarse particles collected near a busy road and at a rural site. Environ Pollut. 2007 Jan;145(2):562-70.（SCI）13. C

14、haung HC*. CpG oligodeoxynucleotides as DNA adjuvants in vertebrates and their applications in immunotherapy. Int. Immunopharmacol. 2006 Oct;6:1586-1596.（SCI）14. Chen CC, Chaung HC, Chung MY, Huang LT*. Menhaden fish oil improves spatial memory in rat pups following recurrent pentylenetetrazole-indu

15、ced seizures. Epilepsy Behav. 2006 May; 8(3):516-21.（SCI）15. Chen DC, Chung YF, Yeh YT, Chaung HC, Kuo FC, Fu OY, Chen HY, Hou MF, Yuan SS*. 2006. Serum adiponectin and leptin levels in Taiwanese breast cancer patients. Cancer Lett. 2006 Jun 8;237(1):109-14.（SCI）16. 蘇正任、陳志誠、吳美莉、陳柏榮、莊秀琪*。2006。二十二碳六烯酸

16、對Pentylenetetrazol (PTZ) 誘發大鼠癲癇造成神經膠細胞凋亡作用及空間記憶之影響。臺灣農業化學與食品科學 44:272-281。17. Chaung HC*, Yang TH, and Chen SJ. 2006. Evaluation of cytotoxicity of nanoparticles using porcine alveolar macrophage as the in vitro screening platform. J. Northwest Sci-Tech University of Agriculture and Forestry 34, 87-

17、92. 18. 莊秀琪*、陳昱彰、鍾文彬、吳彰哲。2005。探討豬隻骨髓造血母細胞試管內分化為樹狀突細胞之細胞特徵。台灣獸醫誌31:195-207. Chung WB*, Chan WH, Chaung HC, Lien Y, Wu CC, Huang YL. 2005. Real-time PCR for quantification of porcine reproductive and respiratory syndrome virus and porcine circovirus type 2 in naturally-infected and challenged pigs. J

18、Virol Methods 124:11-19.（SCI）19. Chen SC*, Chen TH, Wang PC, Chen YC, Huang JP, Lin YD, Chaung HC, Liaw LL. 2003. Metschnikowia bicuspidata and Enterococcus faecium co-infection in the giant freshwater prawn, Macrobrachium rosenbergii. Dis Aquat Organ 55:161-167.（SCI）七、執行計畫(95-98)：1. 探討DHA與磷脂醯絲胺酸對癲癇中樞神經系統之保護機制(97-2320-B-020-003-MY3) 2. 生質柴油發電機排放奈米/超細微粒上多環芳香烴化合物與碳成分粒徑分佈特徵之研究(97-2221-E-020-006-MY3)3. Dectin-1基因多樣性對beta-glucan生物活性之影響(3/3)(97-2321-B-020-002-)4. 以豬in vitro 及in vivo免疫功能評估系統建立beta-glucan免疫調節之篩選平台(3/3)(97-2321-B-020-003-)5. 家禽流行性感冒疫苗DNA佐劑/細胞激素佐劑之研發(97農科-14.6.1-檢-B6(3)大腸桿菌乳房炎時趨化細胞素的