芍药苷论文：从白芍中提取及分离芍药苷和芍药内酯苷

1、芍药苷论文：从白芍中提取及分离芍药苷和芍药内酯苷【中文摘要】白芍是我国传统中药，芍药苷和芍药内酯苷是白芍中的重要活性成分，具有各自独特的药理价值。本文用溶剂提取法从白芍原料药中获得粗提物，通过正交设计法、均匀设计法和球面对称设计法优化提取有效成分的提取液料比例、提取溶剂、提取时间、提取温度;用大孔吸附树脂层析法从白芍粗提物中提取芍药苷和芍药内酯苷混合物，比较两种树脂AB-8D101的提取效果，并考察洗脱液的比例和洗脱体积；用模拟移动床色谱(SMBC精细分离芍药苷和芍药内酯苷,主要进行切换时间、流动相、洗脱流速的选择，并考察了模拟移动床分离运行的稳定性。实验结果表明：白芍中有效成分的溶剂提取的最

2、佳条件为：甲醇浓度为90%提取液料比为1012倍,提取时间为180min,提取温度为40C;用D101树脂对白芍粗提物中芍药苷和芍药内酯苷进一步提取，梯度洗脱方案为：去离子水(2170mL)-20%甲醇水溶液(1250mL)-60%甲醇水溶液(980mL)-纯甲醇(700mL);模拟移动床分离芍药苷和芍药内酯苷的工作参数为：分离柱为4根色谱分离柱(IDxL=1.0x20.0cm),固定相为ODS(2Qxm),工作模式1-1-2,洗脱流动相甲醇：水=30:70(v/v),切换时间25min,进样浓度0.1g/mL,进样流速0.1mL/min,洗脱流速2.0mL/min,冲洗流速4.0mL/min

3、,室温下实验,得到的产品芍药苷的含量大于92%芍药内酯苷的含量大于90%,该SMB分离过程能够稳定运行。【英文摘要】RadixPaeoniaeRubraisonekindofChinesetraditionalmedicine.PeaoniflorinandAlbiflorinareimportantactivecomponentsinRadixPaeoniaeRubra,eachofwhichhasuniquepharmacologicalvalue.Inthispaper,thecrudeextractwasfirstlyobtainedfromRadixPaeoniaeRubrabyso

4、lventextraction.Theextractconditionsincludingliquid-solidratio,extractionsolvent,extractiontimeandextractiontemperaturewereoptimizedbyorthogonaldesign,uniformdesignandsphericalsymmetricdesign,respectively.Then,themixtureofPaeoniflorinandalbiflorinwasseparatedbymacroporousresinfromthecrudeextract,the

5、separationresultswerecomparedusingtwotypesofresin,AB-8andD101.Theeluentandelutionvolumewerealsoselected.Paeoniflorinandalbiflorinwerefurtherpurifiedbysimulatedmovingbedchromatography(SMBC).Switchingtime,themobilephase,elutionflowrate,andotherparameterswereselected,andtheoperationstabilityofSMBwasinv

6、estigated.Experimentalresultsshowedthattheoptimizedextractingconditionswereasfollows:methanolconcentration90%,liquid-solidratio1012,extractiontime180min,extractiontemperature40°C.ThegradientelutionwasusedintheseparationofthetwocomponentsbyD101resin,elutionprogramwasdeionizedwater(2170mL)-20%met

7、hanolinwater(1250mL)-60%methanolinwater(980mL)-Puremethanol(700mL).ThemixtureofPaeoniflorinandAlbiflorinwasfinallyseparatedbysimulatedmovingbed,andthemainprocessparameterswereasfollows:fourchromatographiccolumn(IDxL=1.0x20.0cm),stationaryphasewas20卩mODSpacking;mobilephasewasmethanol:water=3:7(V/V);e

8、luentflowrate2.0mL/min;purgingflowrate4.0mL/min;injectionflowrate0.1mL/min;feedconcentration0.1g/mL;switchingtime25min;operatingatroomtemperature.Undertheaboveconditions,themixturewasseparatedbySMB,andmorethan92%and90%contentofPaeoniflorinandAlbiflorinwereobtained,respectively.TheSMBseparationprocesswasstable.【关键词】芍药苷芍药内酯苷模拟移动床色谱大孔吸附树脂If备注】索购全文在线典加我：139938848同时提供论文辅导写作和学术期刊论文发表服务本文为学术文就总库合作提供无涉版权-作者如有异说谙与总库或学校聡系，【英文关键词】PaeoniflorinAlbiflorinSMBCMacr