分析化学紫外可见分光光度法zwkjC

1、123续前4续前min%11maxmax，shcmE5续前45. 315. 388. 170. 155036127836155036127812AAAAVB，三处最大吸收，定性鉴别：药典规定6续前7续前%06. 01002101 . 1%/101 . 1100/101 . 1143505. 0334%11肾上腺酮mlmgmlglEACcm8lECA定量依据：9续前要求单色光前提：iECAmLgCigW 求含样过程：已知稀释)/(/)(lEAmLgCi100/lALmolCi/或%100%样CCii10练习mLgmLglEAC/0 .20100/00200. 01207414. 011练习mLg

2、mLgCi/1045. 2100/1045. 21207507. 053mLgC/1050. 2100101001050. 252样%0 .98%1001050. 21045. 2%100%5512样CCBi12练习%)0 .764(591. 01001025000.20367255iEAmLmLmLmgmlmLHCL求已知测移取稀至样品稀至吡啶 mLgmLglEACi/1092. 7100/1092. 7764591. 064%0 .99%10025010100100 . 21092. 7%100%26样CCii13条件前提：固定仪器和测定CACKA样样同上条件固定条件查得测定样品曲线分别测

3、定配制标准系列过程：CAACA标样标样标样标样AACCAACC14示例mLmgmLmLmLmg250 . 32550/200. 0样品标样分别移取15续前标样与样品浓度相近；截距为固定仪器和测定条件；前提：0标样和分别测定一定过程：AASiSiAACCsCiCCSiSiAAmm%100%mmii16练习mLgCCii/1 .98518. 0508. 01000100000.25105 . 2%1 .98%100%12标示量标示量iCB17练习1207508. 01050. 2iiClEACmLgCi/1 .98%1 .98%100%12标示量标示量iCB18续前cbaAAAA总定量依据：aaa

4、aaaEACCEA1111由bbbbbbEACCEA2222由bbaaAEAE222111；测定；测定过程：19续前babaaaAEEAE2222111；和测定；测定过程：aaaaaaEACCEA1111由bbaababaCECEAAA22222由baababECEAC22220续前21babababaAEEAEE22221111；和测定；和测定bbaababaCECEAAA11111bbaababaCECEAAA22222bababbabbaaEEEEEAEAC12211221babaabaababEEEEEAEAC1221211222babababaAAAAAA222111babbaaba

5、babaAAAAAAAAA)()(212121aaAAa2121和的等吸收点选bbbbbbbbaCECEEAAA)(2121bbabbbabEAEEAC2123续前bbAAb 2 1 2 1和的等吸收点选aaaaaaabaCECEEAAA)(21 2 1abaaabaaEAEEAC 2 12425续前 倍率因子令KAAbb21)()()()(1212112212aabbababbababaAKAAKAAAAAKAKAAbA1bA2aaaaabaCEKEAKAA)(1212的干扰消除了bCAaba26练习9 .927%11cmEmLgmLgCi/10900. 4100/10900. 419 .927463. 064mLgC/10000. 55052001000.1063样%0 .98%10010000. 510900. 4%100%66样咖啡酸CCi5052001稀释因子注：27练习%11cmE12000 .100120001010%11MEcmmLgmLglETlEACi/10165. 3100/10166.