PFC脑区促进睡眠神经元

1、Corresponding AuthorYang Dan, PhDBS, physics, Beijing UniversityPhD, neurobiology, Columbia UniversityCurrent positionsHoward Hughes Medical InstituteUniversity of California, BerkeleyCurrent projectsNeural circuits controlling sleep.Function of the prefrontal cortex (PFC). 12021/4/26Background Diff

2、erent wavesgalleryhip/rem-sleep-cycle.html22021/4/26Background Sleep cyclegalleryhip/rem-sleep-cycle.html32021/4/26Background POA Lesions in the POA cause sleep impairment. Sleep-active neurons exist in the POA. The sleep-active neurons are spatially intermingled with wake-active neurons.Preoptic ar

3、ea, a region of the hypothalamusmedial preoptic nucleus median preoptic nucleuslateral preoptic nucleusperiventricular preoptic nucleus/wiki/Preoptic_area42021/4/26Background TMNTuberomammillary nucleus, a region of the posterior hypothalamus largely, wake-promoting histaminer

4、gic neurons arousal, learning, memory, sleep and energy balance./wiki/Tuberomammillary_nucleus52021/4/26GABAPOATMNExtended Data Figure 1a62021/4/26GABAPOATMNGABAergic neuronshistaminergic neuronsPOATMNGABAPOATMNExtended Data Figure 1b72021/4/26GABAPOATMN singles out the sleep-

5、promoting neuronsGABAPOATMN96.21.4%95.43.7%Extended Data Figure 2h82021/4/26GABAPOATMN singles out the sleep-promoting neuronsGABAPOATMN73.64.8%Sleep reboundGABAergic neuronshistaminergic neuronsPOATMNGABAPOATMNSleep-promotingExtended Data Figure 2i92021/4/26Two parts of the essay Identification of

6、preoptic sleep neurons using retrograde labelling and gene profiling102021/4/269、 人的价值，在招收诱惑的一瞬间被决定。22.1.1322.1.13Thursday, January 13, 202210、低头要有勇气，抬头要有低气。23:28:4623:28:4623:281/13/2022 11:28:46 PM11、人总是珍惜为得到。22.1.1323:28:4623:28Jan-2213-Jan-2212、人乱于心，不宽余请。23:28:4623:28:4623:28Thursday, January 13

7、, 202213、生气是拿别人做错的事来惩罚自己。22.1.1322.1.1323:28:4623:28:46January 13, 202214、抱最大的希望，作最大的努力。2022年1月13日星期四下午11时28分46秒23:28:4622.1.1315、一个人炫耀什么，说明他内心缺少什么。2022年1月下午11时28分22.1.1323:28January 13, 202216、业余生活要有意义，不要越轨。2022年1月13日星期四23时28分46秒23:28:4613 January 202217、一个人即使已登上顶峰，也仍要自强不息。下午11时28分46秒下午11时28分23:28:

8、4622.1.13Retrograde labelling Causal role: Optogenetic activation or inhibition of GABA POATMN neurons enhances or suppresses sleep.Figure 1Extended data figure 3122021/4/26Retrograde labelling Data analysisActivationInhibitionControlExtended data figure 5132021/4/26Retrograde labelling Functionally

9、 distinct: Optogenetic activation of GABA POA or VGLUT POATMN neurons promotes wakefulness.Figure 3KEY1: TMN projectingKEY2: GABAergic142021/4/26Retrograde labelling Optrode: Measure directly the spiking activity of ChR2-labelled GABA POATMN neurons.Figure 4152021/4/26Retrograde labelling Optrode: M

10、easure directly the spiking activity of ChR2-labelled GABA POATMN neurons.17 identified GABA POATMN neurons 51 unidentified POA unitsFigure 3162021/4/26Gene profilingTRAPTranslating Ribosome Affinity PurificationFigure 4aRNA-seq172021/4/26Gene profilingCCKCRHFigure 4b cTRAP(cholecystokinin)( cortico

11、tropin-releasing hormone)182021/4/26Gene profilingCCKCRHExtended data figure 10192021/4/26Gene profilingRNA-seqTAC1( Preprotachy-kinin-1)&PDYN( ProDynorpy-in)202021/4/26Conclusion Using optogenetic manipulation and recording, virus-mediated circuittracing, and gene profiling, we have characteriz

12、ed a population ofPOA sleep neurons and identified their markers. GABA VS. neuropeptidesOther pathways enhancing sleepSleep-wake circuits212021/4/26Thank you！222021/4/269、 人的价值，在招收诱惑的一瞬间被决定。22.1.1322.1.13Thursday, January 13, 202210、低头要有勇气，抬头要有低气。23:28:4723:28:4723:281/13/2022 11:28:47 PM11、人总是珍惜为得到

13、。22.1.1323:28:4723:28Jan-2213-Jan-2212、人乱于心，不宽余请。23:28:4723:28:4723:28Thursday, January 13, 202213、生气是拿别人做错的事来惩罚自己。22.1.1322.1.1323:28:4723:28:47January 13, 202214、抱最大的希望，作最大的努力。2022年1月13日星期四下午11时28分47秒23:28:4722.1.1315、一个人炫耀什么，说明他内心缺少什么。2022年1月下午11时28分22.1.1323:28January 13, 202216、业余生活要有意义，不要越轨。20

14、22年1月13日星期四23时28分47秒23:28:4713 January 202217、一个人即使已登上顶峰，也仍要自强不息。下午11时28分47秒下午11时28分23:28:4722.1.13谢谢大家谢谢大家9、 人的价值，在招收诱惑的一瞬间被决定。22.1.1322.1.13Thursday, January 13, 202210、低头要有勇气，抬头要有低气。23:28:4723:28:4723:281/13/2022 11:28:47 PM11、人总是珍惜为得到。22.1.1323:28:4723:28Jan-2213-Jan-2212、人乱于心，不宽余请。23:28:4723:28:4723:28Thursday, January 13, 202213、生气是拿别人做错的事来惩罚自己。22.1.1322.1.1323:28:4723:28:47January 13, 202214、抱最大的希望，作最大的努力。20