PFC脑区促进睡眠神经元

1、1会计学PFC脑区促进睡眠神经元脑区促进睡眠神经元Sleep cyclehttp:/ Lesions in the POA cause sleep impairment. Sleep-active neurons exist in the POA. The sleep-active neurons are spatially intermingled with wake-active neurons.Preoptic area, a region of the hypothalamusmedial preoptic nucleus median preoptic nucleuslateral

2、preoptic nucleusperiventricular preoptic nucleus/wiki/Preoptic_areaTMNTuberomammillary nucleus, a region of the posterior hypothalamus largely, wake-promoting histaminergic neurons arousal, learning, memory, sleep and energy balance./wiki/Tuberomammillar

3、y_nucleusExtended Data Figure 1aGABAergic neuronshistaminergic neuronsPOATMNGABAPOATMNExtended Data Figure 1bGABAPOATMN96.21.4%95.43.7%Extended Data Figure 2hGABAPOATMN73.64.8%Sleep reboundGABAergic neuronshistaminergic neuronsPOATMNGABAPOATMNSleep-promotingExtended Data Figure 2iIdentification of p

4、reoptic sleep neurons using retrograde labelling and gene profiling Causal role: Optogenetic activation or inhibition of GABA POATMN neurons enhances or suppresses sleep.Figure 1Extended data figure 3Retrograde labelling Data analysisActivationInhibitionControlExtended data figure 5Retrograde labell

5、ing Functionally distinct: Optogenetic activation of GABA POA or VGLUT POATMN neurons promotes wakefulness.Figure 3KEY1: TMN projectingKEY2: GABAergicRetrograde labelling Optrode: Measure directly the spiking activity of ChR2-labelled GABA POATMN neurons.Figure 4Retrograde labelling Optrode: Measure

6、 directly the spiking activity of ChR2-labelled GABA POATMN neurons.17 identified GABA POATMN neurons 51 unidentified POA unitsFigure 3Gene profilingTRAPTranslating Ribosome Affinity PurificationFigure 4aRNA-seqGene profilingCCKCRHFigure 4b cTRAP(cholecystokinin)( corticotropin-releasing hormone)Gen

7、e profilingCCKCRHExtended data figure 10Gene profilingRNA-seqTAC1( Preprotachy-kinin-1)&PDYN( ProDynorpy-in)Conclusion Using optogenetic manipulation and recording, virus-mediated circuittracing, and gene profiling, we have characterized a population ofPOA sleep neurons and identified their markers. GABA VS. neuropeptidesOther pathways enhancing sleepSleep-wake circuitsThank you！Retrograde labelling Data analysisActi