亚铁氰化钾铁氰化钾放大碳量子点电化学发光对谷胱甘肽的测定表现出良好的灵敏性

1、文献汇报研究背景：研究背景：谷胱甘肽（GSH）是重要的内源性抗氧化剂，用于防御毒素。不正常的含量，减缓小孩的生长、头发脱落、器官损伤，甚至糖尿病、心血管疾病等。因此谷胱甘肽（GSH）的检测至关重要。碳量子点（C-dots）的优点：电化学发光，生物兼容性。 碳量子点（C-dots）的缺点：狭窄的电势窗口，减弱ECL信号。(11-pyrrolyl-1-ylundecyl) triethylammonium tetrafluoroborate (A2)作为阳离子表面活性剂通过静电作用与C-dots结合在一起。亚铁氰化钾和铁氰化钾优点：作为电子注入和空穴注入，能在电化学反应中加快电子的转移。亚铁氰化钾

2、和铁氰化钾在poly(C-dots/A2)能增强ECL信号。实验部分：制备C-dots2.673 g 丙氨酸溶解在30.0 ml 超纯水再加入500 L 乙二胺转入聚四氟乙烯高压釜中 200加热6 h冷却至室温，得到透明黑褐色液体在透析包（1000 MWCO）中透析24 h45下旋转蒸发，得到粉末固体C-Dots固定在玻碳电极上：A2通过声波降解法分散到二次蒸馏水中制得浓度为8 mMC-dotsC-dots/A2声波降解10 minGCE/poly(C-dots/A2）取10 L，放在GCE上，室温干燥 30 min。0-0.9 V；80圈；50 mV s-1。制备GCE/Poly(C-dot

3、s/A2)-Fe(CN)63-/4-GCE/poly(C-dots/A2）GCE/poly(C-dots/A)-Fe(CN)63-/4-浸入含有10 mM K3Fe(CN)6/K4Fe(CN)6 (1:1)0.1 M PBS(pH=7.0)。-0.4-0.7V；60圈；50mV s-1Figure 1. (A) CVs of GCE obtained in 0.1 M PBS (pH =7.0) containing 10 mM K4Fe(CN)6/K3Fe(CN)6 (a) and poly(C-dots/A2)-Fe(CN)63/4 in 0.1 M PBS (pH=7.0) (b) at

4、 a scan rate of 50 mV s1,respectively. (B) CVs of poly(C-dots/A2)-Fe(CN)63/4 in 0.1 M PBS (pH=7.0) at various scan rates. The scan rate from inner to outer is 50 (a), 100 (b), 200 (c), 300 (d), 400 (e), and 500 mV s1 (f). Inset: Plots of anodic and cathodic peak currents vs scan rates. (C) ECLpotent

5、ial curves of poly(C-dots/A2)-Fe(CN)63/4 in 0.05 M Tirs-HCl (pH=7.4) without (a) and with (b) 10 mM K2S2O8 at a scan rate of 100 mV s1. (D) ECL responses of poly(C-dots/A2)-Fe(CN)63/4obtained during a continuous potential scan between 1.75 and 0 V.电化学研究：电化学研究：Figure 2. (A) ECL-time curves of poly(C-

6、dots/A2)-Fe(CN)63/4 in the presence of (aj) 0, 0.1, 0.3, 0.5, 0.7, 1, 2, 4, 7, and 10 M GSH. The electrolyte is Tris-HCl (0.05 M, pH=7.4) with 10 mM K2S2O8 and 0.1 M KCl. (B) The corresponding ECL vs GSH concentration curves. Inset: Linear calibration plot for GSH detection. (C) The ECL response of

7、poly(C-dots/A2)-Fe(CN)63/4 in the presence of interfering substances.Figure 3. SEM (A,C,D) and TEM (B) images for (A) polyA2, (B) Cdots, (C) poly(C-dots/A2), and (D) poly(C-dots/A2) Fe(CN)63/4.Inset shows the photographs of C-dots/A2 solution under visible light (left) and under 365 nm UV light (rig

8、ht).Figure 4. (A) ECL (a) and PL (b) spectrum of the poly(C-dots/A2)-Fe(CN)63/4 system. (B) The effect of different coreactants on the ECL intensity of poly(C-dots/A2)-Fe(CN)63/4: (a) O2, (b) H2O2, (c) cysteine, and (d) K2S2O8. Inset: amplification of lines a, b, and c. (C) ECL response of poly(C-do

9、ts/A2), poly(C-dots/A2)-K4Fe(CN)6, poly(C-dots/A2)-K3Fe(CN)6, and poly(C-dots/A2)-Fe(CN)63/4 in Tris-HCl buffer solution (0.05 M, pH=7.4) with K2S2O8 (10 mM) and poly(C-dots/A2)-Fe(CN)63/4 in Tris-HCl buffer solution (0.05 M, pH=7.4) with K2S2O8 (10 mM) saturated by nitrogen.Figure 5. CVs of the mod

10、ified electrodes recorded in 0.1 M PBS (pH=7.0) for (A) GCE/poly(C-dots/A2) in the absence (a) and presence of O2 (b), (B) GCE/poly(C-dots/A2)-Fe(CN)63/4 in the absence (a) and presence of O2 (b), (C) under N2, GCE/poly(C-dots/A2)-Fe(CN)63/4 in the absence (a) and presence of 10 mM K2S2O8 (b), (D) under ambient atmosphere, GCE/poly(C-dots/A2) Fe(CN)63/4in the absenc