常用酿酒酵母菌株基因型教程文件

1、常用酿酒酵母菌株基因型精品文档Com mon ly used stra insThis page describes some of the most commonly used yeast lab strains. Much of the information is taken fromF.Sherman (2002) Getting started with yeast, Methods Enzymol. 350, 3-41. Other useful papers for strain background information include:Mortimer and Johnst

2、on (1986) Genetics 113:35-43 - thoroughly describes the genealogy of strain S288Cvan Dijken et al. (2000) Enzyme Microb Technol 26:706-714 - compares various characteristics ofcommonly used lab strains?Winzeler et al.(2003) Genetics 163:79-89 - uses SFP (single-feature polymorphisms) analysis to stu

3、dygenetic identity between common lab strainsS288CGenotype: MAT aSUC2 gal2 mal mel flo1 flo8-1 hap1 ho bio1 bio6Notes: Strain used in the systematic sequencing project, the sequence stored in SGD. S288C does not formgalactose anaerobically.The S288C genome was recently resequenced at the Sanger Inst

4、itute .References: Mortimer and Johnston (1986) Genetics 113:35-43.Sources: ATCC:204508BY4743Genotype: MAT a/ ahis3 1/his3 A1 leu2 M/leu2 山 LYS2/lys2 山 met15 M/MET15 ura3 M/ura3 山Notes:Strain used in the systematic deletion project,generated from a cross between BY4741 and BY4742, whichare derived f

5、rom S288C. As S288c, these strains have an allelic variant ofMIP1which increases petite frequency.See Brachmann et al. reference for details.References: Brachmann et al. (1998) Yeast 14:115-32.Sources: Biosystems:YSC1050FY4Genotype: MAT aNotes: Derived from S288C.References: Winston et al. (1995) Ye

6、ast 11:53-55.Brachmann et al. (1998) Yeast 14:115-32.FY1679Genotype: MAT a/ aura3-52/ura3-52 trpl A63/TRP1 Ieu2 A1/LEU2 his3 A200/HIS3 GAL2/GALNotes: Isogenic to S288C; used in the systematic sequencing project, the sequence stored in SGD.References: Winston et al. (1995) Yeast 11:53-55.Sources: EUR

7、OSCARF:10000DAB972Genotype: MAT aX2180-1B trp1 0 rho 0Notes: Isogenic to S288C; used in the systematic sequencing project, the sequence stored in SGD. AB972 is an ethidium bromide-induced rho- derivative of the strain X2180-1B- trp1.References: Olson MV et al. (1986) Proc. Natl. Acad. Sci. USA 83:78

8、26-7830.Sources: ATCC:204511A364AGenotype: MAT a ade1 ade2 ura1 his7 lys2 tyr1 gal1 SUC mal cup BIONotes: Used in the systematic sequencing project, the sequence stored in SGD.References: Hartwell (1967) J. Bacteriol. 93:1662-1670.Sources: ATCC:208526XJ24-24aGenotype: MAT a ho HMa HMt ade6 arg4 -17

9、trp1-1 tyr7-1 MAL2Notes: Derived from, but not isogenic to, S288CReferences: Strathern et al. (1979) Cell 18:309-319DC5Genotype: MAT a leu2-3,112 his3-11,15 can1-11Notes: Isogenic to S288C; used in the systematic sequencing project, the sequence stored in SGD.References: Broach et al. (1979) Gene 8:

10、121-133X2180-1AGenotype: MAT a SUC2 mal mel gal2 CUP1Notes: S288c spontaneously diploidized to give rise to X2180. The haploid segregants X2180-1a and X2180-1b were obtained from sporulated X2180Referen ces: Mortimer and JohnstonSources:ATCC:204504YN N216Genotype: MAT a/ aura3-52/ura3-52 Iys2-8O1 am

11、ber /lys2-801 amber ade2-101 ochre/ade2-101 ochreNotes: Congenic to S288C (see Sikorski and Hieter). Used to derive YSS and CY strains (see Sobel and Wolin).References:Sikorski RS and Hieter P(1989) Genetics 122:19-27.Sobel and Wolin(1999) Mol. Biol. Cell 10:3849-3862.YPH499Genotype: MAT a ura3-52 l

12、ys2-801_amber ade2-101_ochre trp1-出3 his3- A200 leu2- A1Notes: Contains nonrevertible (deletion) auxotrophic mutations that can be used for selection of vectors. Note thattrp1-出3, unlike trp1- A1, does not delete adjacent GAL3 UAS sequence and retains homology to TRP1 selectable marker. gal2-, does

13、not use galactose anaerobically. Derived from the diploid strain YNN216 (Johnston and Davis 1984; original source: M. Carlson, Columbia University), which is congenic with S288C.References: Sikorski RS and Hieter P (1989) Genetics 122:19-27.Sobel and Wolin (1999) Mol. Biol. Cell 10:3849-3862.Johnsto

14、n M and Davis RW (1984) Mol Cell Biol 4(8):1440-8.Sources:ATCC:204679YPH500Genotype: MAT aura3-52 lys2-801_amber ade2-101_ochre trp1-出3 his3- A200 leu2- A1Notes: MAT a strain isogenic to YPH499 except at mating type locus. Derived from the diploid strain YNN216 (Johnston and Davis 1984; original sou

15、rce: M. Carlson, Columbia University), which is congenic with S288C.References: Sikorski RS and Hieter P (1989) Genetics 122:19-27.Sobel and Wolin (1999) Mol. Biol. Cell 10:3849-3862.Johnston M and Davis RW (1984) Mol Cell Biol 4(8):1440-8.Sources: ATCC:204680YPH501Genotype: MAT a/MAT a ura3-52/ura3

16、-52 lys2-801_amber/lys2-801_amber ade2-101_ochre/ade2-101_ochre trp1-63/trp1-出3 his3-应00/his3-应00 leu2- A1/leu2- A1Notes: a/ a diploid isogenic to YPH499 and YPH500 . Derived from the diploid strain YNN216 (Johnston and Davis1984; original source: M. Carlson, Columbia University), which is congenic

17、with S288C.References: Sikorski RS and Hieter P (1989) Genetics 122:19-27.Sobel and Wolin (1999) Mol. Biol. Cell 10:3849-3862.Johnston M and Davis RW (1984) Mol Cell Biol 4(8):1440-8.Sources:ATCC:204681Sigma 1278BNotes: Used in pseudohyphal growth studies.Detailed notes about the sigma strains have

18、been kindly provided byCora Styles.Granek and Magwene , PLoS Genet. 2010 Jan 22;6(1):e1000823, established that certain lineages of theSigma1278B background contain a nonsense mutation in RIM15, a G-to-T transversion at position 1216 that converts a Gly codon to an opal stop codon. This rim15 mutati

19、on interacts epistatically with mutations in certain other genes to affect colony morphology.Annotation of the Sigma1278b genome and information about the systematic deletion collection can be foundhere.SK1Genotype: MAT a/ aHO gal2 cup S can1 R BIONotes: Commonly used for studying sporulation or mei

20、osis. Canavanine-resistant derivative.The SK1 genome was sequenced at the Sanger Institute .References: Kane SM and Roth J. (1974) Bacteriol. 118: 8-14Sources: ATCC:204722CEN.PK (aka CEN.PK2)Genotype: MAT a/ aura3-52/ura3-52 trp1-289/trp1-289 leu2-3_112/leu2-3_112 his31/his3 A1 MAL2-8C/MAL2-8CSUC2/S

21、UC2Notes: CEN.PK possesses a mutation in CYR1 (A5627T corresponding to a K1876M substitution near the end of the catalytic domain in adenylate cyclase which eliminates glucose- and acidification-induced cAMP signalling anddelays glucose-induced loss of stress resistance ( Vanhalewyn et al., 1999 ; D

22、umortier et al., 2000 ).References: van Djken et al. (2000) Enzyme Microb Technol 26:706-714Sources: EUR0SCARF:30000DW303Genotype: MAT a/MAT a eu2-3,112 trp1-1 can1-100 ura3-1 ade2-1 his3-11,15 phi+Notes:W303 also contains abud4mutation that causes haploids to bud with a mixture of axial and bipolar

23、 buddingpatterns. In addition, the original W303 strain contains therad5-535allele. As S288c, W303 has an allelic variantofMIPI which increases petite frequency.The W303 genome was sequenced at the Sanger Institute .References:W303 constructed by Rodney Rothstein ( seedetailed notes from RR and Step

24、han Bartsch )bud4 info:rad5-535Voth et al. (2005) Eukaryotic Cell, 4:1018nfo: Fan et al. (1996) Genetics 142:749-28.Sources: Biosystems:YSC1058W303-1AGenotype: MAT a leu2-3,112 trp1-1 can1-100 ura3-1 ade2-1 his3-11,15Notes:W303-1A possesses aybp1-1mutation (I7L, F328V, K343E, N571D) which abolishes

25、Ybp1p function,increasing sensitivity to oxidative stress.References: W303 constructed by Rodney Rothstein ( see detailed notes from RR and Stephan Bartsch ).ybp1-1 info: Veal et al. (2003) J. Biol. Chem. 278:30896-904.Sources:Biosystems:YSC1058W303-1BGenotype: MAT a su2-3,112 trp1-1 can1-100 ura3-1

26、 ade2-1 his3-11,15References: W303 constructed by Rodney Rothstein ( see detailed notes from RR and Stephan Bartsch ).Sources: Biosystems:YSC1058W303-K6001Genotype: |mAT a; ade2-1, trp1-1, can1-100, leu2-3,112, his3-11,15, GAL, psi+, ho:HO:CDC6 (at HO), cdc6:hisG,ura3:URA3 GAL-ubiR-CDC6 (at URA3) Re

27、ferences:K6001 was developed byBobola et alin Kim Nasmyths lab (PMID: 8625408), and has become acommon model in yeast aging research (PMID: 15489200). Its genome has been sequenced by Timmermann et al(PMID: 20729566)D273-10BGenotype: MAT amalNotes: Normal cytochrome content and respiration; low freq

28、uency of rho-. This strain and its auxotrophic derivatives were used in numerious laboratories for mitochondrial and related studies and for mutant screens. Good respirer thats relatively resistant to glucose repression.References: Sherman, F. (1963) Genetics 48:375-385.Sources: ATCC:24657FL100Genot

29、ype: MAT aReferences: Lacroute, F. (1968) J. Bacteriol. 95:824-832.Sources: ATCC: 28383SEY 6210/SE Y6211Genotype: MAT a/MAT a leu2-3,112/leu2-3,112 ura3-52/ura3-52 his3-A200/his3- A200 trp1- A901/trp1- A901ade2/ADE2 suc2- A9/suc2- A9 GAL/GAL LYS2/lys2-801Notes:SEY6210/SEY6211, also known as SEY6210.

30、5, was constructed by Scott Emr and has been used in studiesof autophagy, protein sorting etc. It is the product of crossing with strains from 5 different labs (Gerry Fink, Ron Davis,David Botstein, Fred Sherman, Randy Schekman). It has several selectable markers, good growth properties andgood spor

31、ulation.References: Robinson et al. (1988) Mol Cell Biol 8(11):4936-48Sources:ATCC:201392SEY6210Genotype: MAT aleu2-3,112 ura3-52 his3- A200 trp1- A901 suc2-出 lys2-801; GALNotes: SEY6210 is a MATalpha haploid constructed by Scott Emr and has been used in studies of autophagy, protein sorting etc. It

32、 is the product of crossing with strains from 5 different labs (Gerry Fink, Ron Davis, David Botstein, Fred Sherman, Randy Schekman). It has several selectable markers and good growth properties.References: Robinson et al. (1988) Mol Cell Biol 8(11):4936-48Sources: ATCC:96099SEY6211Genotype: MAT a l

33、eu2-3,112 ura3-52 his3- A200 trp1- A901 ade2-101 suc2- A9; GALNotes:SEY6211 is a MATa haploid constructed by Scott Emr and has been used in studies of autophagy, proteinsorting etc. It is the product of crossing with strains from 5 different labs (Gerry Fink, Ron Davis, David Botstein, FredSherman,

34、Randy Schekman). It has several selectable markers and good growth properties.References: Robinson et al. (1988) Mol Cell Biol 8(11):4936-48Sources: ATCC:96100JK9-3dThere are a, alpha and a/alpha diploids of JK9-3d with the following genotypes:Genotypes: JK9-3da MAT a leu2-3,112 ura3-52 rme1 trp1 hi

35、s4JK9-3d a has the same genotype as JK9 -3da with the exception of the MAT locusJK9-3da/ a is homozygous for all markers except mating typeNotes: JK9-3d was constructed by Jeanette Kunz while in Mike Halls lab. She made the original strain while Joe Heitman isolated isogenic strains of opposite mati

36、ng type and derived the a/alpha isogenic diploid by mating type switching. It has in its background S288c, a strain from the Oshima lab, and a strain from the Herskowitz lab. It was chosen because of its robust growth and sporulation, as well as good growth on galactose (GAL+) (so that genes under c

37、ontrol of the galactose promoter could be induced). It may also have a SUP mutation that allows translation through premature STOP codons and therefore produces functional alleles with many point mutations.References:Heitman et al.(1991a) Science 253(5022):905-9 andHeitman et al.(1991b) Proc Natl Acad Sci U S A88(5):1948-52RM11 -1aG