测序通用引物序列.doc

测序通用引物序列常见载体的测序引物：Primer of Vector:Vector：Primer(F)；Primer(R)pACT T7 T3pACT2 GAL4 AD pACT2-RpAS2-1 GAL4 BD pAS2-1.RpB42AD pB42ADF pB42ADRpBACPAK8 BAC1 BAC2pBK-CMV T7 T3PBS(SK/KS)/M13- M13F/T7 T3/M13RpBV220 PBV220F PBV220RpCAMBIA 1301(1300) P1 P2pCAMBIA 2300 M13R(-48) M13F(-47)PCANTB5E S1/M13R S6pCAT3-enhancer RVP3 此载体无反向引物pcDNA3.0 CMV-F/T7 SP6/BGHpcDNA3.1 T7 BGHpcDNA4 T7/CMV-F BGHpcDNA6 T7/CMV-F(J21025在T7前面） BGHpcDNAII T7 SP6pCE2.1 M13F M13RpCEP4 pCEP-F EBV-RpCF-T M13F M13RpCI T7（17Base） 此载体无反向引物pCI-neo T7（17Base） T3pCMS-EGFP T7 T3pCMV-3Tag-4A T3 /PFLAG-CMV-F T7pCMV5 pCMV5F pCMV5RpCMV5-Flag pCMV5F pCMV5RpCMV-MYC/HA pCMV-F pCMV-RpCMV-Sport M13F/T7 SP6/M13RpCMV-Tag(KAN+) T3 T7pCR2.1-TOPO M13F/T7 M13RpCR3.1 T7 BGHpCS2 SP6 T7pDonar M13F M13RpDONR221 M13F M13RpDrive T7 SP6pDRIveR(KAN+) T7 SP6pDsRED1-C1 pDsRED-ex-C1-F pEGFP-N-3(距离很近,一般不用)pDsRED2-C1(KAN+) pDsRED-ex-C1-F pEGFP-N-3pDSRED-N1(KAN+) pEGFP-N-5 PDSRED-N-RpECFP-C pEGFP-C-5 pECFP-C-3pECFP-N1 pEGFP-N-5 pEGFP-N-3pEF/myc/ER pEF-F pCDNA3.1RpEGFP-C(KAN+) pEGFP-C-5 pEGFP-C-3pEGFP-N(KAN+) pEGFP-N-5 pEGFP-N-3pENTR/D-TOPO(KAN+) M13F M13RpET-*(His)(KAN+) T7 T7 TerpET22(a,b,c)(AMP+) T7 T7 TerpET28,30,24,49(KAN+) T7 T7 TerpET32(a,b,c)(AMP+) T7/S.tag T7 TerpET50(amp+) T7/s.tag T7TERpET44(AMP+) T7/s.tag T7 TerpEYFP-N1 pEGFP-N-5 pEGFP-N-3pEYFP-C1 pEGFP-C-5 pEGFP-C-3pFLAG-CMV pFLAG-CMV -F pFLAG-CMV -RpGAD424 GAL4 AD 3ADpGADGH GAL4 AD 3ADpGADT7-Rec GAL4 AD/T7 3ADpGAPZa-A a-FACTOR 3AOXpGBKT7(Kana+) T7 3BDpGEM-T(-Easy) M13F/T7 SP6/M13RpGEX-(*)T pGEX-4T-5 pGEX-4T-3pGL2 GLP1 GLP2pGL3 RVP3 GLP2(RVP4)(没有特别说明用GLP2)PinpointTM Vector Pinpoint Primer SP6pIRES PIRES-F/T7 T3/PIRES-R (J40720)pIRES2-EGFP pIRES2-EGFP.P5 pIRES2-EGFP.P3pLXSN pLXSN-F pLXSN-RpMAL-c2E MalE primer M13F(-47)pMAL-C2x P5 P3pMAL-p2X MalE primer M13F(-47)PMD18-T M13R(-48) M13F(-47)PMD19-T M13F(-47) M13R(-48)pPIC9K(AMP+、ZEO+） 5AOX/a-factor 3AOXpPICZa 5AOX/PPICZa-F 3AOXpPROEXHTA M13R(-48) 无反向引物pQE30or40 pQE30-F pQE-R:pRSET T7 T7TERpSilencer3.1-H1 hygro M13F(-47) 3.0REVpSILENCEV2.0-U6 T7 2.0REVpSK01-T M13F M13RpSK-CMV(KAN+) T7 T3pSP72 T7 SP6pSport1 SP6/M13F(-47) T7/M13RpSUPER T7 T3pTA2 M13F/T7(优先使用M13F） T3/M13RpT-Adv M13F /T7 M13RpTARGET TM pTarget.F(在T7前面）/T7 pTarget.RpTO-T7 T7 T7TERpTRC99a-c pTRC99C-F pTRC99C-RpTriPLEx2 5pTriPLEx2 T7pTWIN-1 T7 T7TER(客户确认再用）pUC18(19)/118(119) M13F(-47) M13R(-48)pUCm-T M13F/T7 M13RpVAX1 T7 BGHpWSK29 T7/M13F/M13F(-47) T3/M13R/M13R(-48)pXT7 T7 SP6pYES2 T7 pYes2.RpLEXA pLEXA-F pLEXA-RpLNCX pLNCX-F pLNCX-RpRECEIVER CMV-F 此载体无反向引物pSHUTTLE-CMV PSHUTTLE-CMV-F PSHUTTLE-CMV-RpSP64/65 SP6 此载体无反向引物pSTBlue-1 T7 SP6pT7Blue(R) T7 M13F(-47)引物名称 序列(5-3)：M13R：CAG GAA ACA GCT ATG ACCM13F：TGT AAA ACG ACG GCC AGTM13F(-47)：CGC CAG GGT TTT CCC AGT CAC GACM13R(-48)：AGC GGA TAA CAA TTT CAC ACA GGAM13(-96)：CCC TCA TAG TTA GCG TAA CGSP6：ATT TAG GTG ACA CTA TAGT7：TAA TAC GAC TCA CTA TAG GGT7 terminator：TGC TAG TTA TTG CTC AGC GGT3：ATT AAC CCT CAC TAA AGG GApGEX-4T-5：GGG CTG GCA AGC CAC GTT TGG TGpGEX-4T-3：CCG GGA GCT GCA TGT GTC AGA GGGLp1：TGT ATC TTA TGG TAC TGT AAC TGGLp2：CTT TAT GTT TTT GGC GTC TTC CARVp3：CTA GCA AAA TAG GCT GTC CCRVp4：GAC GAT AGT CAT GCC CCG CGpcDNA3.1R：TAG AAG GCA CAG TCG AGGPinPoint primer： CGT GAC GCG GTG CAG GGC GpCMV-F： TCT AAA AGC TGC GGA ATT GTpCMV-R： TCCAAACTCATCAATGTATCpTRC99C-F: TTG CGC CGA CAT CAT AACpTRC99C-R: CTGCGTTCTGATTTAATCTGpCEP-F: AGA GCT CGT TTA GTG AAC CGEBV-R : GTG GTT TGT CCA AAC TCA TCpIRES2-EGFP.P5: GTA GGC GTG TAC GGT GGG AGpIRES2-EGFP.P3: AAC GCA CAC CGG CCT TAT TC3AD: AGA TGG TGC ACG ATG CAC AGCMV -F： CGC AAA TGG GCG GTA GGC GTGS1： CAA CGT GAA AAA ATT ATT ATT CGCS6： GTA AAT GAA TTT TCT GTA GTA GG5AOX1：GAC TGG TTC CAA TTG ACA AGC3AOX1：GCA AAT GGC ATT CTG ACA TCC-Factor：TAC TAT TGC CAG CAT TGC TGCGAL4 AD：TAC CAC TAC AAT GGA TGpACT2-R：GTGCACGATGCACAGTTGAApB42ADF：CCA GCC TCT TGC TGA GTG GAG ATGpB42ADR：AAG CCG ACA ACC TTG ATT GGA GpEGFP-N-5：TGG GAG GTC TAT ATA AGC AGA GpEGFP-N-3：CGT CGC CGT CCA GCT CGA CCA GpEGFP-C-5： CAT GGT CCT GCT GGA GTT CGT GpEGFP-C-3 ：TAT GGC TGA TTA TGA TCA GTPBV220F：AAG AAG GGC AGC ATT CAA AGPBV220R：CTG CGT TCT GAT TTA ATC TGU6：ATG GAC TAT CAT ATG CTT ACC GTA2.0rev primer：AGG CGA TTA AGT TGG GTA3.0rev：GAG TTA GCT CAC TCA TTA GGCS.tag：GAA CGC CAG CAC ATG GAC5TriplEx2：CTC CGA GAT CTG GAC GAG CRVP4：GAC GAT AGT CAT GCC CCG CGRVP3：CTA GCA AAA TAG GCT GTC CCpQE30-R：GTT CTG AGG TCA TTA CTG GpQE30-F：TGA GCG GAT AAC AAT TTC ACpEF-F：TCA AGC CTC AGA CAG TGG TTCpDSRED-N1-R：TGA AGC GCA TGA ACT CCT TGpDsRED-express-C1-F：TCC CAC AAC GAG GAC TAC ACpCMV5R： ATT ATA GAG GAC ACC TAG TCpCMV5F： TTC CAA AAT GTC GTA ATA ACP5： TGC GTA CTG CGG TGA TCA ACP3： CTG CAA GGC GAT TAA GTT GGBAC2： ACG CAC AGA ATC TAG CGC TTBAC1： AAC CAT CTC GCA AAT AAA TA3BD： TAA GAG TCA CTT TAA AAT TTG TAT ACpTarget.F： CCA GGA TTT TCC CAG TCA CpTarget.R： GGC TTT ACA CTT TAT GCT TCT7(17base)： ACA TCC ACT TTG CCT TTC TCpYes2.R： TCG GTT AGA GCG GAT GTGGAL4 BD： TCA TCG GAA GAG AGT AGpAS2-1.R： AAG AGT TAC TCA AGA ACA AGA ApFlag-CMV-F: GGT AGG CGT GTA CGG TGGpFlag-CMV-R: GCA CTG GAG TGG CAA CTTpIRES-F: CTT ACT GAC ATC CAC TTT GCpIRES.R: CAC TGC ATT CTA GTT GTG GTP1： CCA GGC TTT ACA CTT TAT GCP2： GCG ATT AAG TTG GGT AAC GCpLEXA-F： CGT CAG CAG AGC TTC ACC ATpLEXA-R： TAA AAC CTA AGA GTC ACT TTpLNCX-F： AGC TCG TTT AGT GAA CCG TCA GAT CGpLNCX-R： ACC TAC AGG TGG GGT CTT TCA TTC CCpLXSN-F： CTT GAA CCT CCT CGT TCG ACpLXSN-R： GTT GCT GAC TAA TTG AGA TGpSHUTTLE-CMV-F： GGT CTA TAT AAG CAG AGG TGpSHUTTLE-CMV-R： GTG GTA TGG CTG ATT ATG ATC AGMalE Primer： GGT CGT CAG ACT GTC GAT GAA GCC引物名称序列(5-3)M13RCAG GAA ACA GCT ATG ACCM13FTGT AAA ACG ACG GCC AGTM13F(-47) CGC CAG GGT TTT CCC AGT CAC GACM13R(-48)AGC GGA TAA CAA TTT CAC ACA GGAM13(-96) CCC TCA TAG TTA GCG TAA CGSP6 ATT TAG GTG ACA CTA TAGT7TAA TAC GAC TCA CTA TAG GGT7 terminator TGC TAG TTA TTG CTC AGC GGT3 ATT AAC CCT CAC TAA AGG GApGEX-4T-5GGG CTG GCA AGC CAC GTT TGG TGpGEX-4T-3CCG GGA GCT GCA TGT GTC AGA GGGLp1TGT ATC TTA TGG TAC TGT AAC TGGLp2CTT TAT GTT TTT GGC GTC TTC CARVp3CTA GCA AAA TAG GCT GTC CCRVp4GAC GAT AGT CAT GCC CCG CGpcDNA3.1RTAG AAG GCA CAG TCG AGGPinPoint primerCGT GAC GCG GTG CAG GGC GpCMV-FTCT AAA AGC TGC GGA ATT GTpCMV-R TCCAAACTCATCAATGTATCpTRC99C-F: TTG CGC CGA CAT CAT AACpTRC99C-R: CTGCGTTCTGATTTAATCTGpCEP-F: AGA GCT CGT TTA GTG AAC CGEBV-R :GTG GTT TGT CCA AAC TCA TCpIRES2-EGFP.P5:GTA GGC GTG TAC GGT GGG AGpIRES2-EGFP.P3: AAC GCA CAC CGG CCT TAT TC3AD: AGA TGG TGC ACG ATG CAC AGCMV -FCGC AAA TGG GCG GTA GGC GTGS1 CAA CGT GAA AAA ATT ATT ATT CGCS6 GTA AAT GAA TTT TCT GTA GTA GG5AOX1 GAC TGG TTC CAA TTG ACA AGC3AOX1 GCA AAT GGC ATT CTG ACA TCC-FactorTAC TAT TGC CAG CAT TGC TGCGAL4 ADTAC CAC TAC AAT GGA TGpACT2-RGTGCACGATGCACAGTTGAApB42ADF: CCA GCC TCT TGC TGA GTG GAG ATGpB42ADR: AAG CCG ACA ACC TTG ATT GGA GpEGFP-N-5: TGG GAG GTC TAT ATA AGC AGA GpEGFP-N-3: CGT CGC CGT CCA GCT CGA CCA GpEGFP-C-5: CAT GGT CCT GCT GGA GTT CGT GpEGFP-C-3 : TAT GGC TGA TTA TGA TCA GTPBV220F: AAG AAG GGC AGC ATT CAA AGPBV220R:CTG CGT TCT GAT TTA ATC TGU6ATG GAC TAT CAT ATG CTT ACC GTA2.0rev primer:AGG CGA TTA AGT TGG GTA3.0rev: GAG TTA GCT CAC TCA TTA GGCS.tagGAA CGC CAG CAC ATG GAC5TriplEx2CTC CGA GAT CTG GAC GAG CRVP4GAC GAT AGT CAT GCC CCG CGRVP3 CTA GCA AAA TAG GCT GTC CCpQE30-RGTT CTG AGG TCA TTA CTG GpQE30-FTGA GCG GAT AAC AAT TTC ACpEF-FTCA AGC CTC AGA CAG TGG TTCpDSRED-N1-RTGA AGC GCA TGA ACT CCT TGpDsRED-express-C1-FTCC CAC AAC GAG GAC TAC ACpCMV5R ATT ATA GAG GAC ACC TAG TCpCMV5F TTC CAA AAT GTC GTA ATA ACP5TGC GTA CTG CGG TGA TCA ACP3CTG CAA GGC GAT TAA GTT GGBAC2ACG CAC AGA ATC TAG CGC TTBAC1AAC CAT CTC GCA AAT AAA TA3BD: TAA GAG TCA CTT TAA AAT TTG TAT ACpTarget.FCCA GGA TTT TCC CAG TCA CpTarget.RGGC TTT ACA CTT TAT GCT TCT7(17base)ACA TCC ACT TTG CCT TTC TCpYes2.RTCG GTT AGA GCG GAT GTGGAL4 BDTCA TCG GAA GAG AGT AGpAS2-1.RAAG AGT TAC TCA AGA ACA AGA ApFlag-CMV-F:GGT AGG CGT GTA CGG TGGpFlag-CMV-R:GCA CTG GAG TGG CAA CTTpIRES-F:CTT ACT GAC ATC CAC TTT GCpIRES.R:CAC TGC ATT CTA GTT GTG GTP1CCA GGC TTT ACA CTT TAT GCP2GCG ATT AAG TTG GGT AAC GCpLEXA-FCGT CAG CAG AGC TTC ACC ATpLEXA-RTAA AAC CTA AGA GTC ACT TTpLNCX-FAGC TCG TTT AGT GAA CCG TCA GAT CGpLNCX-RACC TAC AGG TGG GGT CTT TCA TTC CCpLXSN-FCTT GAA CCT CCT CGT TCG ACpLXSN-RGTT GCT GAC TAA TTG AGA TGpSHUTTLE-CMV-FGGT CTA TAT AAG CAG AGG TGpSHUTTLE-CMV-RGTG GTA TGG CTG ATT ATG ATC AGMalE PrimerGGT CGT CAG ACT GTC GAT GAA GCC引物名称引物序列3AOX5-GGC AAA TGG CAT TC TGA CAT-33AD5-AGA TGG TGC ACG ATG CAC AG-33BD5-TAA GAG TCA CTT TAA AAT TTG TAT C-35AOX15-GAC TGG TTC CAA TTG ACA AGC-3A-FACTOR5-TAC TAT TGC CAG CAT TGC TGC-3BAC15-AAC CAT CTC GCA AAT AAA TA-3BAC25-ACG CAC AGA ATC TAG CGC TT-3BGH rev5-TAG AAG GCA CAG TCG AGG C-3CMV-F5-CGC AAA TGG GCG GTA GGC GTG-3CMV-R5-GTT CAC GGT GCC CTC C-3DsRed1-C-r5-AGC TGG ACA TCA CCT CCC ACA ACG-3DsRed1-N-f5-GTA CTG GAA CTG GGG GGA CAG-3EBV Reverse5-TGT GGT TTG TCC AAA CTC AAT C-3EBV-R5-GTG GTT TGT CCA AAC TCA TC-3GAL4 AD-F5-TAC CAC TAC AAT GGA TG-3GAL4 BD5-TCA TCG GAA GAG AGT AG-3GLP15-TGT ATC TTA TGG TAC TGT AAC TG-3GLP25-CTT TAT GTT TTT GGC GTC TTC CA-3M13(-96)5-CCC TCA TAG TTA GCG TAA CG-3M13F5-TGT AAA ACG ACG GCC AGT-3M13F（-47）5-CGC CAG GGT TTTC CCA GTC ACG AC-3M13R5-CAG GAA ACA GCT ATG ACC-3M13R（-48）5-AGC GGA TAA CAA TTT CAC ACA GGA-3P249905-TCATCGGAAGCGAGTAG-3P314305-CGT TTT AAAACCTAAGAGTCAC-3P125845-TTT TCA GTA TCT ACG AT-3P171105-TAC CAC TAC AAT GGA TG-3PAS2-1F5-TCA TCG GAA GCG AGT AG-3PAS2-1R5-CGT TTT AAA ACC TAA GAG TCA C-3PB42ADF5-CCA GCC TCT TGC TGA GTG GAG ATG-3PB42ADR5-AAG CCG ACA ACC TTG ATT GGA G-3pbd-gal4-f5-GCC TCT AAC ATT GAG ACA GC-3pbd-gal4-r5-AAG AGT TAC TCA AGA ACA AGA A-3pbi-121(35s)5-GAC GCA CAA TCC CAC TAT CC-3PBV220F5-AAG AAG GGC AGC ATT CAA AG-3PBV220R5-CTG CGT TCT GAT TTA ATC TG-3PCDNA3.0-F25-CTC TGG CTA ACT AGA GAA CC-3PCDNA3.0-R25-GGC AAC TAG AAG GCA CAG TC-3PCDNA3.1F5-CTA GAG AAC CCA CTG CTT AC-3PCDNA3.1R5-TAG AAG GCA CAG TCG AGG-3pCEP Frorward5-AGC AGA GCT CGT TTA GTG AAC CG-3PCEP-F5-AGA GCT CGT TTA GTG AAC CG-3PCMV-F5-TCT AAA AGC TGC GGA ATT GT-3PCMV-F5-TTC CAA AAT GTC GTA ATA AC-3PCMV-R5-TCC AAA CTC ATC AAT GTA TC-3PCMV-R5-ATT ATA GAG GAC ACC TAG TC-3PDONR-F5-TCG CGT TAA CGC TAG CAT GGA TCT C-3PDONR-R5-GTA ACA TCA GAG ATT TTG AGA CAC-3PEF-F5-TCA AGC CTC AGA CAG TGG TTC-3PEGFP-C-35-TAT GGC TGA TTA TGA TCA GT-3PEGFP-C-55-CAT GGT CCT GCT GGA GTT CGT G-3PEGFP-N-35-CGT CGC CGT CCA GCT CGA CCA G-3PEGFP-N-55-TGG GAG GTC TAT ATA AGC AGA G-3pETup5-ACG ATG CGT CCG GCG TAG AGG A-3PGEX-35-CCG GGA GCT GCA TGT GTC AGA GG-3PGEX-55-GGG CTG GCA AGC CAC GTT TGG TG-3PIRES2-EGFP-F5-GTA GGC GTG TAC GGT GGG AG-3PIRES3-EGFP-R5-AAC GCA CAC CGG CCT TAT TC-3pMAL-C2X-F5-TGC GTA CTG CGG TGA TCA AC-3pMAL-C2X-R5-CTG CAA GGC GAT TAA GTT GG-3PQE30F5-TGA GCG GAT AAC AAT TTC AC-3PQE30R5-GTT CTG AGG TCA TTA CTG G-3pTARGET-SEQ5-TTA CGC CAA GTT ATT TAG GTG ACA-3PTRC99C-F5-TTG CGC CGA CAT CAT AAC-3PTRC99C-R5-CTG CGT TCT GAT TTA ATC TG-3PTRCHISA-F5-CAT GGT ATG GCT AGC ATG AC-3PTRCHISA-R5-CAG GCT GAA AAT CTT CTC TC-3RVP35-CTA GCA AAA TAG GCT GTC CC-3RVP45-GAC GAT AGT CAT GCC CCG CG-3S.TAG5-CGA ACG CCA GCA CAT GGA CA-3S15-CAA CGT GAA AAA ATT ATT ATT CGC-3S65-GTA AAT GAA TTT TCT GTA TGA GG-3SP65-ATT TAG GTG ACA CTA TAG-3T35-ATT AAC CCT CAC TAA AGG GA-3T75-TAA TAC GAC TCA CTA TAG GG-3T7（17BASE）5-ACA TCC ACT TTG CCT TTC TC-3T7TER5-TGC TAG TTA TTG CTC AGC GG-3TRXF5-CAT ATG AGC GAT AAA ATT ATT CAC-3TRXR5-GGA TCC CTT GTC ATC GTC ATC ACC A-3malEfor5-GGT CGT CAG ACT GTC GAT GAA GCC-3pTrcHis-fw5-TTA AAG AGG TAT ATA TTA ATG TAT CG-3Xpressfor5-AGC ATG ACT GGT GGA CAG-3pTrcHis-rv5-ATC TGT ATC AGG CTG AAA ATC-3N-CMV-305-ATA ACC CCG CCC CGT TG-3C-CMV-245-TTA GGA CAA GGC TGG TGG-3CMV-Profor5-ATG GGC GGT AGG CGT G-3pCAMBIA1301-5(ECORI)5-CCA GGC TTT ACA CTT TAT GC-3pCAMBIA1301-3(HindIII)5-GCG ATT AAG TTG GGT AAC GC-3T7 EEV5-ATG TCG TAA TAA CCC CGC CCC G-3pDBLeu-55-GAA TAA GTG CGA CAT CAT CAT C- 3pDBLeu-35-CAG ATG GAA CGG TCT TTA AAT G-3HGHrev5-CTG GGG AGG GGT CAC AGG G-3PSG5(BAMHI)-F5-GCT GGT TAT TGT GCT GTC TC-3PSG5-R(SV40)5-AAA CCA CAA CTA GAA TGC AGT-3M13R(-26)5-CAG GAA ACA GCT ATG AC-3pBRforBam5-CTT GGA GCC ACT ATC GAC-3pBRrevBam5-GGT GAT GTC GGC GAT ATA GG-3pBRforEco5-AAT AGG CGT ATC ACG AGG C-3pBRrevHind5-GCG TTA GCA ATT TAA CTG TG-3 c-mycrev5-CTC TTC TGA GAT GAG TTT TTG-3 pGAL4-ADrv5-GAA CTT GCG GGG TTT TTC-3 pGAL4-BDrv5-AAT CAT AAG AAA TTC GCC C-3 pMD19T490R5-CACAGGAAACAGCTATGACC-3Psos55-CCA AGA CCA GGT ACC ATG-3Psos35-GCC AGG GTT TTC CCA GT-3Primer TriplEx-LD-5 5-CTC GGG AAG CGC GCC ATT GTG TTG G-3 Primer TriplEx-LDrv-3 5-ATA CGA CTC ACT ATA GGG CGA ATT GGC-3 UB-F5-TCA GTG TTA GAC TAG TAA ATT-327F5-AGA GTT TGA TCC TGG CTC AG-31492R5-GGY TAC CTT GTT ACG ACT T-3