常用同位素性质.doc

常用同位素性质列表放射性核素半衰期射线类型3H12.4 years-7Be53.3 daysECa11C20.5 min+14C5760 year-22Na2.6 year+24Na14.8 h-28Mg21.4 h-31Si170 min-32P14.3 days-33P25 days-35S87.4 days-36Cl310 000 years-38Cl38.5 min-42K12.4 h -45Ca165 days-47Ca4.54 days-51Cr28 days52Mn5.8 days+54Mn310 days55Fe2.94 years57Co270 days58Co72 days+59Fe46.3 days-60Co5.3 years-63Ni100 years-64Cu12.8 h-+65Zn250 days+75Se121 days76As26.8 h-82Br36 h-86Rb18.7 days-89Sr51 days-90Sr28.5 years-99Mo68 h-109Cd462 daysECa110Ag249.8 days-111Ag7.47 days-113Sn115.1 daysECa115Cd44.6 days-125I60.0 daysECa131I8.04 days-133Ba10.8 yearsECa134Cs2.06 years-135I9.7 h-137Cs30.17 years-185W75.1 days-197Hg64.4 hECa198Au2.696 days-199Au3.13 days-203Hg46.6 days-同位素检测方法同位素介质检测方法3H琼脂糖凝胶闪烁计数聚丙酰胺凝胶 硝酸纤维素膜，滤纸14C/35S琼脂糖凝胶fluorography(for max. sensitivity)or聚丙酰胺凝胶 放射自显影（可获较高的分辩率）硝酸纤维素膜，滤纸放射自显影32P/125I琼脂糖凝胶放射自显影（用增感屏）聚丙酰胺凝胶 (for max. sensitivity) or direct硝酸纤维素膜，滤纸放射自显影（可获较高的分辩率）同位素在试验中的应用应用同位素检测方法DNA/RNA 打点杂交32P放射自显影（用增感屏）DNA 序列测定32P放射自显影35S放射自显影原位杂交32P放射自显影35S放射自显影3H放射自显影体外蛋白质合成14C/35S闪烁计数3H闪烁计数噬斑和菌落的筛选32P放射自显影（用增感屏）35S放射自显影Southern and Northern 杂交32P放射自显影（用增感屏）35S放射自显影（用增感屏）Western blots3H闪烁计数14C/35S放射自显影125I放射自显影（用增感屏）常用同位素理化性质，检测方法单位换算 Curies 和 d . p . m 的换算.1 Curie (Ci) =2.221012 d . p . m .1 milliCurie (mCi) =2.22109 d . p . m .1 microCurie (m Ci) =2.22106 d . p . m .1 nanoCurie (nCi) = 2.22103 d . p . m .1 picoCurie (pCi) =2.22 d . p . m .Curies 和 Becquerels的换算 1 Ci = 3.71010 Bq =37GBq (gigaBq)1 mCi = 3.710 7 Bq =37MBq (megaBq)1 m Ci = 3.710 4 Bq =37kBq (kiloBq)1 GBq =2.710 -4 Ci =27.027mCi(mil