渔业资源与渔场学课件早期发育实例论文

Spawning,earlydevelopment,andfirstfeedinginthelemonpeel

angelfishCentropygeflavissimus(蓝眼黄新娘)Aquaculture253(2006)270–2781.Introduction棘蝶鱼科Pomacanthidae:7属，70个种，分布广泛。蓝眼黄新娘2.Materialsandmethods2.1.AnimalsAllspeciesofCentropygestudiedtodatefromharemgroupsofonemaleandtwotofourfemales。lemonpeelangelfishCentropygeflavissimusweremaintainedinpairsin300Ltanksandfedtwicedaily(8amand4pm)withcommercialflakefoodandrawshrimporfish;Photoperiodandtemperatureweremanipulatedusinglighttimersandheatpumpstosimulateseasonalchanges.winterseason:10hlightand20C;summer:13hlightand28–30C;Springandfallconditions:11–12hlightand24–26C.Salinitywasmaintainedat32–36ppt,pH8.2,NO2andNH3小于0.03ppm.2.2.BehaviouralobservationsSpawningactivitywasrecordedondigitalvideoTape；Recordingsweremadeduringthefinal90minoflighteachevening.Aresearcherstartedtherecorderandimmediatelylefttheroomsoasnottodisturbthespawningpair。2.3.Embryocollection,development,andhatchingrate150目筛绢网集卵；5微米滤水，26度下于2L容器中孵化，PH8.2，盐度32-36。2.4Larvalrearing150L玻璃钢水槽培养。孵化6天后才微充气。池中光线较弱，加绿藻至看不到池底止。从孵化6天后开始换水，每天早晨15%。每天加几滴potassiumiodine（钾碘）。2.5.Evaluationofliveprey对不同饵料培养效果进行评价。2.6.Experimentaldesign分组进行不同饵料下成活率实验。6个培养容器，共1200粒卵用于温度和营养对成活率影响的实验研究。每天投喂两次，每个容器约200尾。3.Results3.1.CourtshipandspawningA：产卵前；B：产卵；C：产卵后3.2.Fertilizationandhatchrate受精率大约90%。受精卵孵化率98%。26度下约15小时孵化。3.3.Embryodevelopment见下图。A：受精后1小时B：受精后3小时，原肠期C：受精后8小时D：受精后10.5小时E：受精后14.5小时，孵化前。3.4.LarvaldevelopmentA：