Tat-NTS-peptide-生命科学试剂-MCE

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemETat-NTSpeptideCat.No.:HY-P10275分⼦式:C₁₂₅H₂₁₂N₅₄O₂₄分⼦量:2855.37Sequence:Tyr-Gly-Arg-Lys-Lys-Arg-Arg-Gln-Arg-Arg-Arg-Arg-Ser-Phe-Pro-His-Leu-Arg-Arg-Val-Phe-NH2SequenceShortening:YGRKKRRQRRRRSFPHLRRVF-NH2作⽤靶点:Others作⽤通路:Others储存⽅式:PleasestoretheproductundertherecommendedconditionsintheCertificateofAnalysis.BIOLOGICALACTIVITY⽣物活性Tat-NTSpeptide细胞穿透肽，具有神经保护作⽤。Tat-NTSpeptide能特异性的抑制ANXA1的核易位，减少缺⾎区的神经元凋亡(apoptosis)。并且Tat-NTSpeptide能减少脑缺⾎性梗死体积，可以⽤于缺⾎性卒中的研究[1]。体外研究Tat-NTSpeptide(20μM)blocksANXA1nuclearmigrationinneuronalcellswithoutaffectingthenucleartranslocationofotherproteins[1].Tat-NTSpeptide(20μM)inhibitsthebindingofANXA1top53,alleviatesneuronalapoptosisinducedbyoxygenglucosedeprivation/reperfusion(OGD/R),andhasneuronalcytoprotectiveeffects[1].Tat-NTSpeptide(20μM)cansignificantlyimprovetheviabilityofneuronalcellsafterOGD/R[1].WesternBlotAnalysis[1]CellLine:neuronalcellsConcentration:20μMIncubationTime:Result:SignificantlydecreasedthelevelofANXA1proteininthenuclearfraction,andhadnoimpactonthesubcellularlocalizationofp65andβ-catenin.SignificantlydecreasedBidproteinlevels.SignificantlydecreasedOGD/R-triggeredtBidexpressionandcaspase-9,PARP,1/2MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemEcaspase-3cleavagebuthadlittleeffectontheactivationofcaspase-8.RT-PCR[1]CellLine:neuronalcellsConcentration:20μMIncubationTime:Result:SignificantlydecreasedBidmRNAlevels.体内研究Tat-NTSpeptide(0.5-10mg/kg;nilateralIntracerebroventricular(i.c.v.)Injection;singledose)reducestheinteractionbetweenANXA1andimportinβinC57BL/6micewithmiddlecerebralarteryocclusion(MCAO)andpreventsthenucleartranslocationofANXA1invivo.Tat-NTSpeptidealleviatesneuronalapoptosisafterischemicinjuryinmice,exhibitingneuroprotectiveeffectsagainstcerebralischemia[1].AnimalModel:MCAOC57BL/6micemodel[1]Dosage:0.5mg/kg,1mg/kg,2mg/kg,5mg/kg,10mg/kg.Administration:UnilateralIntracerebroventricular(i.c.v.)Injection:Singledose.Afterundergoing1-hourmiddlecerebralarteryocclusion(MCAO)Result:SignificantlyinhibitedtheproteinleveloftBid,cleavedcaspase-9,cleavedPARP,andcaspase-3,andhadlittleeffectontheactivationofcaspase-8.Reducedtheinfarctvolumeandimprovedneurologicaloutcomesfollowingfocalischemicinjury.REFERENCES[1].LiX,etal.Anovelcell-penetratingpeptideprotectsagainstneuronapoptosisaftercerebralischemiabyinhibitingthenucleartranslocationofannexinA1.CellDeathDiffer.2019Jan;26(2):260-275.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalappl