DNA实验-免疫细胞表面抗原分子CD家族对照表CD1-CD24

免疫细胞表面抗原分子CD家族对照表(CD1CD247)ExalphaBiologicalsproducesandsellsproductsforLifeScienceResearch,includingmonoclonalandpolyclonalantibodies,foruseinimmunohistochemistry,westernblot,ELISAandFlowCytometry、Weofferover800productsand,throughcollaborationswithotherscientistsaswellasourowninhouseR&Dprograms,Exalphacontinuestobringleadingedgeproductstomarket、OurfamilyofCDantibodiesbinesqualityandperformancewithreasonablepricestomakeourCDantibodiesthebestvalueinthemarkettoday、OurfamilyofProliferationproductsoffereasyallinonekitsorstandaloneantibodiestoquantitateproliferatingcells、Takealookatourwebsiteorcallusforadditionalinformation、Note:

noinformationisavailableonCDw145,CD181,CD182,CD185194,CD196199,CD211,CD214216,CD218220,

CD237BackgroundCD:

clusterdesignationofmonoclonalantibodies(clustersofdifferentiation)Designatedatthe1stto7thWorkshopsonInternationalHumanLeukocyteDifferentiationAntigens

Thelastconferencewasin2000、Thenextconferenceisin2004、

HLDAWorkshopsaretheprimarymechanismtocharacterizeleukocytesurfaceantigenicmoleculesandepies;erythroidantigensarenowincluded

For1stto6thworkshops,antibodiesweresubmittedtotheorganizinglaboratory,codedandsenttoparticipatinglaboratoriesfortestingagainst

various

\o""cell

types、Forthe7thworkshop,aCDdesignationcouldbeestablishedforamoleculeifitsgenehas

beenclonedandatleastonespecificmonoclonalantibodyhad

beenstudiedintheWorkshopInterpretationshouldbebasedoncellulardistributionofstaining,proportionofpositivelystainedcells,stainingintensityandcutofflevels、CD1FamilyofnonpolymorphicMHCclassIlikeglycoproteinsAlsomemberofimmunoglobulinsuperfamilyOnchromosome1q2223(notMHClinked)Has5differentsubsets,allnoncovalentlyassociatedwith12kdbeta2microglobulinFunction:

restrictTcellresponsestocertainantigens;maymediatethymicTcelldevelopmentPositivestaining(normal):

corticalthymocytes(70%),activatedTcells,Langerhanscells,interdigitatingdendriticcellsPositivestaining(disease):

preTALLwithcorticalthymocytephenotype;LangerhanscellhistiocytosisNegativestaining:

matureperipheralTcellsCD1aPositivestaining(normal):

Dendriticcellsindermis/epidermisofbenigninflammatoryskindisordersPositivestaining(disease):

Langerhanscellhistiocytosis(fairlyspecific),myeloidleukemias,someBcellmalignancies;dendriticcellsinmostperipheralcutaneousTcelllymphomas,

AJCP2001;116:72Negativestaining:

normalBcells,mostcutaneousperipheralBcelllymphomas(?reflectsreplacementofreactivepatterncontainingdendriticcellswithaneoplasticpatternofBcells)Microimages:

LangerhanscellhistiocytosisMicroimages(AJSPsubscribers):

pulmonaryLangerhanscellhistiocytosisMicroimages(HumPathsubscribers):

LangerhanscellhistiocytosisReferences:

AJSP2001;25:630CD1bPositivestaining(disease):

myeloidleukemiasandsomeBcellmalignanciesNegativestaining:

normalBcellsCD1cPositivestaining(normal):

subsetofnormalperipheralBcellsPositivestaining(disease):

myeloidleukemiasandsomeBcellmalignancies

Negativestaining:

normalBcellsCD1dPositivestaining(normal):

thymus(lowlevels),bowelCD1eCD2AkaErosettereceptor,LFA2(leukocytefunctionantigen)Function:

bindsCD58/LFA3onantigenpresentingcells,andinducescostimulatorysignalsinTcellsAlsoregulatesTandNKmediatedcytolysis,inhibitsapoptosisofactivatedperipheralTcells,mediatesTcellcytokineproduction,regulatesTcellanergyPositivestaining(normal):

thymocytes(95%),matureperipheralTcells(almostall),NKcells(8090%),thymicBcells(50%)Microimages:

extranodalNK/Tcelllymphoma,nasaltypeCD2RCD2epiesrestrictedtoactivatedTcellsPositivestaining:

activatedTcells,?NKcellsCD3AkaOKT3Function:

plex(5chains)ofintegralmembraneglycoproteinsassembledasaplex;haslongcylasmictailwithantigenrecognitionactivationmotif;plexisthendownregulatedAlsosubdividedintodelta,epsilon,gammasubtypesCylasmicexpressionatearlyTcelldifferentiation,thenmembranousexpressionMostspecificTcellantibodyPositivestaining(normal):

thymocytes,peripheralTcells,NKcells;alsoPurkinjecellsofcerebellumPositivestaining(disease):

80%ofTcelllymphomasNegativestaining:

gammadeltaTcellreceptors,mostBcelllymphomasMicroimages:

CD3epsilontesticularNK/Tcelllymphoma(figure3D)Microimages(AJSPsubscribers):

achalasia,posttransplantlymphoproliferativediseaseinliverReferences:

AJSP2001;25:1413CD4AkaOKT4,Thelper/inducerOnchromosome#12pNonpolymorphousglycoproteinsbelongingtoimmunoglobulinsuperfamilyServesasHIVreceptoronTcells(asdochemokinereceptorsCCR5andCXCR4),macrophages,brainCD4+TcellsarekilledbyHIVCoreceptorinMHCclassIIrestrictedantigeninducedTcellactivationBindstononpolymorphicregionofclassImolecules;mayincreaseavidityofcellcellinteractionsPositivestaining(normal):

thymocytes(8090%),matureTcells(65%,ThelperandCD4/CD8+thymocytes),macrophages,Langerhanscells,dendriticcells,granulocytesPositivestaining(disease):

pityriasislichenoidesMicroimages:

acutedemyelinatingdisease,extranodalNK/Tcelllymphoma,nasaltypeCD5Belongstoancientscavengerreceptorfamily

IsphysicallyandfunctionallycoupledwithTcellreceptorzetaCD3signaltransducerplexCD5+Bcellsproduce“generalistantibodies”polyreactivelowaffinity“natural”antibodiestoexogenousantigens(tetanustoxoid,lipopolysaccharide)aswellasautoreactiveantibodies(ssDNA,thyroglobulin,insulin)Note:sharksonlyhavepolyreactiveIgMNote:monoreactiveIgGisproducedby<0、1%ofcirculatingBcells,frompositiveselectionandsomatic

pointmutationFirstlineofdefenseagainstantigens;havealowactivationthreshold;aretheonlylineofdefenseforthosewhocannotproducespecificantibodyProduceantibodiesusinggermline(nonmutated)configurationofgenesegments,usuallyIgMProductionelevatedinrheumatoidarthritis(2752%ofcirculatingBcellsvs、20%normal)CD5mayserveasadualreceptor,givingeitherstimulatoryorinhibitorysignalsdependingbothonthecelltypeandthedevelopmentstagePositivestaining(normal):

Bcellsofmantlezoneofspleenandlymphnodes;Bcellsinperitonealandpleuralcavities;almostallTcells;Infetus,mostBcellsinspleenandcordbloodareCD5positivePositivestaining(disease):

BcellCLL/SLL,mantlecelllymphoma,mostTmalignancies,thymiccarcinomas(70%)Negativestaining:

spindlecellthymomas,MALTlymphoma,follicularlymphomaMicroimages:

extranodalNK/Tcelllymphoma,nasaltype,mantlecelllymphoma(figure3D)CD6Adhesionmoleculemediatingthebindingofdevelopingthymocyteswiththymicepithelialcells

Maybeinvolvedinautoimmunityandgraftvs、hostdisease(GVHD)AntibodiestoCD6areusedtodepleteTcellsfrombonemarrowtransplantstopreventGVHDPositivestaining(normal):

lowlevelsonimmaturethymocytes,highlevelsonmaturethymocytes

CD7MembraneglycoproteinandFcreceptorforIgM

HomologoustoTCRgamma,IgkappaMembraneexpressionearlyduringTontogeny,beforeTCRrearrangement,persistsuntilterminalstagesofTcelldevelopmentLowerexpressioninmemoryTcellsvs、naiveTcellsPositivestaining(normal):

matureperipheralTcells(85%),postthymicTcells(majority),NKcells(majority),somemyeloidcellsPositivestaining(disease):

TcellALL;AML(especiallyM4/M5),chronicmyelogenousleukemia,blastsintransientmyeloproliferativedisorderNegativeexpression:

BcellALL,Sezarysyndrome,adultTcellleukemia/lymphomaMicroimages:

extranodalNK/Tcelllymphoma,nasaltypeCD8AkaOKT8,Tcellsuppressor/cytotoxiccellsOnchromosome#2MHCclassIrestrictedreceptor;bindstononpolymorphicregionofclassImolecules;mayincreaseavidityofcellcellinteractionsAssociatedwithlymphoepitheliomalikecarcinomaoflung(AJSP2002;26:715)Positivestaining(normal):

Tcells(2535%ofmatureperipheralTcells,mostcytotoxicTcells,CD4/CD8+thymocytes);NKcells(30%whicharealsoCD3negative);corticalthymocytes(7080%),epidermotrophiclymphocytesinmycosisfungoides(AJSP2002;26:450)Microimages:

lymphoepitheliomalikecarcinomaofcervixfigure3,acutedemyelinatingdiseaseMicroimages(ModPathsubscribers):

nodalcytotoxicTcelllymphomaReference:

ModPath2002;15:1131CD9MaymediateplateletactivationandaggregationAntibodiesareusedtopurgebonemarrowpriortoperipheralstemcellbonemarrowtransplantViralcoreceptorPositivestaining(norm