不同来源间充质干细胞标志物汇总（全）

不同来源间充质干细胞标志物汇总(全)(图片来源网络)间充质干细胞(mesenchymalstemcells,MSC)是一种源于中胚层的早期干细胞，具有多向分化潜能，特定的条件下可分化为骨细胞、软骨细胞和神经细胞等，支持造血，具备低免疫原性和免疫调节活性，具有广泛的科研和临床应用价值。主要存在于结缔组织和器官间质中，以骨髓中含量最为丰富，少量存在于血液及其他组织中。近年来随着基础科研和临床研究的不断深入，MSC在医学领域的应用价值也得到了极大肯定，但仍然存在一些问题，如：缺少特异性表面标志物，鉴定困难；多次传代后分化能力不能保留；诱导分化调控机制尚不明确等，都是有待于进一步研究和讨论的。MSC主要关键基因和表面分子包括CD73、CD90、CD105，表达极少或无表达CD11b、CD19、CD34、CD45和HLA-DR，其中不同来源之间的MSC标志物存有一定差异性，这里列出来供大家参考。外周血(Peripheralderivedblood)来源[1]阳性:CD105,CD90,CD73,CD73,CD44,CD90.1,CD29,CD105,CD106,CD140a阴性：CD34,CD19,CD11b骨髓(Bonemarrow)来源[2]阳性：CD105,CD13,CD140b,CD147,CD151,CD276,CD29,CD44,CD47,CD59,CD73,CD81,CD90,CD98阴性：CD14,CD31,CD34,CD45皮肤(Skin)/包皮(foreskin)来源[3]阳性：CD29,CD44,CD73,CD90,CD105,vimentin阴性：CD34,CD45,HLADR脂肪(Adipose)来源[4]阳性：CD9,CD29,CD44,CD54,CD73,CD90,CD105,CD106,CD146,CD166阴性：CD14,CD31,CD34,CD45,CD133,CD144,HLA-DR,STRO-1尿液（Urine）来源[5,6]阳性：CD29,CD44,CD54,CD73,CD90,CD105,CD166,STRO-Oct-4,Klf-4,Sox-17,vimentin阴性：CD41,HLA-DR心（Heart）来源[7]阳性：CD44,CD105,CD29,CD90阴性：CD14,CD45,CD34,CD31牙（Dental）来源[8,9]阳性：CD13,CD29,CD44,CD49,CD73,CD90,CD146,STRO-1,Oct-3/4,NANOG,SSEA-3阴性：CD14,CD31,CD34,CD45,HLADR骨骼肌（Skeletalmuscle）来源[10]阳性：CD29,CD44,CD49E,CD56,CD73,CD90,CD105,HLA-I阴性：CD34,CD45胰腺（Pancreas）来源[11]阳性：CD105,CD90,CD73,CD44,CD29,CD13,nestin,vimentin,CD146,NG2,a-SMA,PDGF-R[3阴性：CD31,CD34,andCD45,CK19,CA19.9肺（Lung）来源[12]阳性：CD73,CD90,andCD105,vimentin,prolyl-4-hydroxylase阴性：CD14,CD34,CD45胚胎干细胞（ESC）[13]阳性：CD29,CD44,CD73,CD105,SSEA-4阴性：CD34,CD45,HLADR诱导多能干细胞（iPSC）[14]阳性：CD29,CD44,CD166,CD73,CD105,KDR,MSX2阴性：CD34,CD45,HLADR沃顿果冻(Whartonjelly)来源[15]阳性：CD44,CD73,CD90,CD105,CD166阴性：CD14,CD34,CD45胎盘(Placenta)来源[16,17]阳性：CD105, CD73,CD90c-kit,Thy-1,Oct-4,SOX2,hTERT,SSEA-1,3,4,TRA-1阴性：CD34,CD45,CD14orCD11b,CD19,HLA-DR脐带(Umbilicalcord)来源[18]阳性:CD73,CD90,CD105,Oct-4,Nanog,ABCG2,Sox-2,Nestin阴性：CD34,CD45,CD19,HLA-DR绒毛(Chorionicvilli)来源[19]阳性：CD44,CD117,CD105,a-SMA,CD49,CD146,CD106,CD166,Stro-1,vWF阴性：CD34,CD45,CD19,HLA-DR绒毛膜(Chorionicmembrane)来源[20]阳性：CD44,CD49,CD56,CD73,CD90,CD105阴性：CD45,CD34,CD14,CD31,EPCAM,HLA-DR脐带血(Cordblood)来源[17]阳性：CD29,CD73,CD105,CD44,Oct-4,Sox-1,Sox2,NANOG,ABCG2,Nestin阴性：CD34,CD45子宫内膜(子宫内膜)来源[21]阳性：CD73,CD90,CD105,CD166,HLA-ABC,Oct-4阴性：CD14,CD34,CD45,HLA-DR羊膜(Amnioticmembrane)来源[22,23]阳性：CD73,CD90,CD105,Oct-4,SSEA-4,Tra-1阴性：CD11b,CD14,CD19,CD79a,CD34,CD45,HLA-DR羊水(Amnioticfluid)来源[24]阳性：CD73,CD90,CD105,CD166,MHCclassI,Oct-4,EA-1阴性：CD45,CD40,CD34,CD14,HLA-DR参考文献：LiS,HuangK-J,WuJ-C,HuMS,SanyalM,HuM,LongakerMT,LorenzHPPeripheralblood-derivedmesenchymalstemcells:candidatecellsresponsibleforhealingcritical-sizedcalvarialbonedefects.StemCellsTranslMed.2015;4(4):359-68.AmatiE,PerbelliniO,RottaG,BernardiM,ChieregatoK,SellaS,RodeghieroF,RuggeriM,AstoriG.High-throughputimmunophenotypiccharacterizationofbonemarrow-andcordblood-derivedmesenchymalstromalcellsrevealscommonanddifferentiallyexpressedmarkers:identificationofangiotensinconvertingenzyme(CD143)asamarkerdifferentiallyexpressedbetweenadultandperinataltissuesources.StemCellResTher.2018;9(1):10.ParkJR,KimE,YangJ,LeeH,HongSH,WooHM,ParkSM,NaS,YangSR.Isolationofhumandermisderivedmesenchymalstemcellsusingexplantsculturemethod:expansionandphenotypicalcharacterization.CellTissueBank.2015;16(2):209-18.SchafflerA,BuchlerC.Concisereview:adiposetissue-derivedstromalcells—basicandclinicalimplicationsfornovelcell-basedtherapies.StemCells.2007;25(4):818-27.ZhangD,WeiG,LiPZhouX,ZhangY.Urine-derivedstemcells:anovelandversatileprogenitorsourceforcell-basedtherapyandregenerativemedicine.GenesDis.2014;1(1):8-17.PavathuparambilAbdulManaphN,Al-HawwasM,BobrovskayaL,CoatesPT,ZhouX-F.Urine-derivedcellsforhumancelltherapy.StemCellResTher.2018;9(1):189.PilatoCA,StadiottiI,MaioneAS,SaverioV,CattoV,TundoF,DelloRussoA,TondoC,PompilioG,CasellaM,etal.Isolationandcharacterizationofcardiacmesenchymalstromalcellsfromendomyocardialbiopticsamplesofarrhythmogeniccardiomyopathypatients.JVisExp.2018;132:57263.HuangGTJ,GronthosS,ShiS.Mesenchymalstemcellsderivedfromdentaltissuesvs.thosefromothersources:theirbiologyandroleinregenerativemedicine.JDentRes.2009;88(9):792-806.Ledesma-MartinezE,Mendoza-NunezVM,Santiago-OsorioE.Mesenchymalstemcellsderivedfromdentalpulp:areview.StemCellsInt.2016;2016:4709572.CamernikK,MihelicA,MihalicR,MaroltPresenD,JanezA,TrebseR,MarcJ,ZupanJ.Skeletal-muscle-derivedmesenchymalstem/stromalcellsfrompatientswithosteoarthritisshowsuperiorbiologicalpropertiescomparedtobone-derivedcells.StemCellRes.2019;38:101465.MAhmedS,El-BadriN.Pancreaticmesenchymalstromalcells:characteristicsandpossibleorigin.LiverPancreaticSci.2016;1(1).MartinuT,PalmerSM,OrtizLA.Lung-residentmesenchymalstromalcells.AmJRespirCritCareMed.2011;183(8):968-70.HawkinsKE,CorcelliM,DowdingK,RanzoniAM,VlahovaF,HauKL,HunjanA,PeeblesD,GressensF,HagbergH,etal.Embryonicstemcell-derivedmesenchymalstemcells(MSCs)haveasuperiorneuroprotectivecapacityoverfetalMSCsinthehypoxic-ischemicmousebrain.StemCellsTranslMed.2018;7(5):439-49.XuM,ShawG,MurphyM,BarryF.Inducedpluripotentstemcellderivedmesenchymalstromalcellsarefunctionallyandgeneticallydifferentfrombonemarrow-derivedmesenchymalstromalcells.StemCells.2019;37(6):754-65.PittengerMF,MackayAM,BeckSC,JaiswalRK,DouglasR,MoscaJD,MoormanMA,SimonettiDW,CraigS,MarshakDR.Multilineagepotentialofadulthumanmesenchymalstemcells.Science.1999;284(5411):143-7.MatikainenT,LaineJ.Placenta--analternativesourceofstemcells.ToxicolApplPharmacol.2005;207(2Suppl):544-9.delaTorreP,JesusPerez-LorenzoMI,FloresA.Humanplacenta-derivedmesenchymalstromalcells:areviewfrombasicresearchtoclinicalapplications;2019.IwataniS,YoshidaM,YamanaK,KurokawaD,KurodaJ,ThwinKKM,UemuraS,TakafujiS,NinoN,KodaT,etal.Isolationandcharacterizationofhumanumbilicalcord-derivedmesenchymalstemcellsfrompretermandterminfants.JVisExp.2019;143.DivyaMS,RoshinGE,DivyaTS,RasheedVA,SanthoshkumarTR,ElizabethKE,JamesJ,PillaiRM.Umbilicalcordblood-derivedmesenchymalstemcellsconsistofauniquepopulationofprogenitorsco-expressingmesenchymalstemcellandneuronalmarkerscapableofinstantaneousneuronaldifferentiation.StemCellResTher.2012;3(6):57.GonzalezPL,CarvajalC,CuencaJ,Alcayaga-MirandaF,FigueroaFE,BartolucciJ,Salazar-AravenaL,KhouryM.Chorionmesenchymalstemcellsshowsuperiordifferentiation,immunosuppressive,andangiogenicpotentialsincomparisonwithhaploidenticalmaternalplacentalcells.StemCellsTranslMed.2015;4(10):1109-21.ChengY,LiL,