抗氧化青苹果醋与葡萄多酚对小鼠尿酸血症的预防作用

抗氧化物苹果醋与葡萄多酚对小鼠高尿酸血症的预防作用指导老师：林明栋、余剑平组员：刘嘉伟、张剑威、程慧华、漆微韡、李鹏亮——05物理小组前言

高尿酸与痛风

高尿酸的治疗

本实验的方向

苹果醋与葡萄多酚

材料与方法材料与方法材料与方法实验记录组别性别原始编号分组日体重取血日体重灌胃时间造模时间注射肝素时间取血时间组内编号性别原始编号分组日体重取血日体重灌胃时间造模时间注射肝素时间取血时间组内编号空白雄12930.2——8:559:001雌128.927.9——9:459:5010193233.1——9:059:102253131.3——9:5510:1092433.233.5——9:159:203223.926.5——10:0510:3082034.334.5——9:259:3042429.830——10:1510:5071234.935.5——9:359:4052028.229.3——10:2511:106造模629.230.6—19:1020:0520:106233032—19:0019:5520:0051430.333—19:2020:1520:2072927.527.6—18:5019:4519:5042933.336.6—19:3020:2520:30892730.5—18:4019:3519:4031534.436.8—19:4020:3520:409728.929.6—18:3019:2519:3022635.636.2—19:5020:4520:50101132.334.3—18:2019:1519:2012021年6月18日实验记录组别性别原始编号分组日体重取血日体重灌胃时间造模时间注射肝素时间取血时间组内编号性别原始编号分组日体重取血日体重灌胃时间造模时间注射肝素时间取血时间组内编号高苹果醋雄273034.813:0014:0014:5515:001雌2727.629.813:5014:5015:4515:5012530.432.813:1014:1015:0515:102302727.314:0015:0015:5516:002233233.513:2014:2015:1515:203430.231.514:1015:1016:0516:103934.536.313:3014:3015:2515:304528.328.714:2015:2016:1516:204735.838.313:4014:4015:3515:4052633.830.514:3015:3016:2516:305低苹果醋30303115:3016:3017:2517:306222929.514:4015:4016:3516:406283136.315:4016:4017:3517:4071027.829.314:5015:5016:4516:507232.333.615:5016:5017:4517:508827.128.215:0016:0016:5517:0081333.536.216:0017:0017:5518:009213433.215:1016:1017:0517:109113634.816:1017:1018:0518:10101728.52715:2016:2017:1517:20102021年6月19日实验记录组别性别原始编号分组日体重取血日体重灌胃时间造模时间注射肝素时间取血时间组内编号性别原始编号分组日体重取血日体重灌胃时间造模时间注射肝素时间取血时间组内编号高多酚雄223132.19:5510:5511:5011:551雌628.128.49:3010:3011:2511:3011833.335.310:0011:0011:550:0022828.528.79:3510:3511:3011:352434.235.910:0511:0512:0012:053183030.39:4010:4011:3511:40353539.410:1011:1012:050:1041931.9289:4510:4511:4011:4541634.534.810:1511:1512:1012:1551232.231.39:5010:5011:4511:505低多酚1025.42510:2011:2012:150:2061426.627.410:4511:4512:4012:456332.529.110:2511:2512:2012:257328.327.810:5011:5012:450:507833.536.310:3011:3012:250:3081329.229.810:5511:5512:5012:5582134.837.510:3511:3512:3012:359153030.411:0012:0012:551:0091734.535.110:4011:4012:350:4010163233.711:0512:0513:0013:05102021年6月20日实验结果性别空白组测定结果造模组结果高苹果醋组结果低苹果醋组结果高多酚组结果低多酚组结果组内编号尿酸μmol/LMDAnmol/mL组内编号尿酸μmol/LMDAnmol/mL组内编号尿酸μmol/LMDAnmol/mL组内编号尿酸μmol/LMDAnmol/mL组内编号尿酸μmol/LMDAnmol/mL组内编号尿酸μmol/LMDAnmol/mL雄1121.737.710940.716.41712.619.010622.912.01505.016.110875.411.92114.946.79827.516.22404.021.09707.011.52704.219.19939.916.43121.740.58934.214.43670.620.58723.913.83698.616.48956.716.34101.439.57927.816.44572.417.37782.812.44726.719.77673.412.85128.436.96927.815.15448.919.26751.915.35620.112.56925.911.9雌6118.337.95931.013.86566.718.65101514.76704.214.45920.313.17114.946.94850.212.07552.717.54886.613.37676.218.64892.215.28138.640.73908.412.08477.021.73760.312.38718.212.73684.615.89114.936.22685.310.39735.116.12746.313.69648.117.22715.412.310158.834.31840.511.810561.118.11998.811.210454.516.61667.713.4原始数据实验结果GroupUricacidμmol/LMDAnmol/mL空白123.35±15.7939.72±4.21造模877.33±80.2a13.84±2.22a高苹果醋570.11±110.04b18.90±1.77a低苹果醋799.61±127.55c13.02±1.36a高葡萄多酚645.58±93.93b16.31±2.51a低葡萄多酚825.15±123.06c13.91±1.81aa.与空白组相比，P<0.01b.与造模组相比，P<0.01c.与造模组相比，P>0.05两组间比较采用独立样本t检验实验结果实验结果讨论讨论参考文献[1]徐立，时乐.小鼠代谢性高尿酸血症模型的复制方法初探.中国比较医杂志20061:1[2]金沈锐，郑军.小鼠高尿酸血症模型初探成都中医药大学学报199922(1):49-52[3]金莹，孙爱东.苹果多酚对小鼠的抗氧化作用的研究中国食物与营养20061:31-33[4]王海东，葛飞.金钱