连接互相匹配的粘性末端产生新的酶切位点

连接互相匹配的粘性末端，产生新的酶切位点通常连接互相匹配的粘性末端可以产生新的前切位点。以下组合是通过计算机程序计算出来的，尽管我们希望能尽量保证其准确性，但有些未经实验证实，因此不能保证100%的可行性。如果有同裂酶存在，则只列出其中的•个前。本表中列出的内切酶均为已商品化的内切酶。识别序列不够严谨的内切酶（例如，识别多个序列的陶）只在括号内标出相关的序列，但注意这些酶不只切割该序列。表示连接产物不能被再切割。酶连接到可进行切割的酶Acc65l(G/GTACC)Ban1(G/GTACC)Acc65I,Ban1,Kpn1,NiaIV,Rsa1BsiW1,BsrG1Rsa1Acc1(GT/CGAC)AciLAcl1,BsaH1(GR^CGYC),HinP1I,HpaII,Nar1—(GT/CGAC)Cla1,BstB1,Taq1Taq1Aci1(C/CGC)Acc1(GT/CGAC),Acl1,Cla1,BstB1,Taq1-BsaH1(GR/CGCC),HinP1I,NariAcilHpaIIHpaIIAcl1(AAj'CGTT)AccI(GT/CGAC),AciI.Cla1,BstB1,HinP!I,HpaII,Nar1,Taq1-AfiIIIPci1(A.'CATGT)AfiII(A/CATGT),BspH1,Neo1,Sty1(C/CTAGG)NiaIIIPpuM1(RG/GWCCY)AvaII,RsrllAvaII.Sau96I(GG/GTCCY)AvaII,RsrIIAvaII,BsmF1,NiaIV.Sau961(GG/GACCY)AvaII.RsrIIAvaII.NiaIV.Sau961PspOM1(G/GGCCC)Eae1(Y/GGCCR),Eag1HaeIII,Sau961NotlAci1,Fnu4HLHaeIII.Sau961Pstl(CTGCA/G)BsiHKA1,Bsp1286I(GTGCAC)BsglNsiI—SbflPstlPvu1(CGAT/CG)AsiSIDpn1,Pvu1PacIMse1BsiEI(CGAT/CG)BsiE1,DpnII,Pvu1Rsrll(CG/GWCCG)AvaII,PpuMI(RG^GACCY)AvaII.Sau961PpuMI(RG/GACCY)AvaII.NiaIV.Sau961PpuMI(RG/GTCCY)AvaII.BsmF1,NiaIV,Sau961Sac1(GAGCT/C)BanII(GAGCT/C).BsiHKAI,Bsp1286I(GAGCT/C)Alu1,BanII,BsiHKA1,Bsp1286I,SacISacII(CCGC/GG)BsiEI(CGGC/CG)AciISal1(G/TCGAC)Ava1(C/TCGAG),Xho1Taq1Sbtl(CCTGCA/GG)BsiHKA1,Bsp12861(GTGCAC)Bsg1Nsi1-PstlPstlSfcl(c.qrGCAG)ApaL1Bsg1SgrAI(CR/CCGGYG)SeeBsrF1Spe1(A/CTAGT)AvrII,Nhe1,Sty1(C/CTAGG),Xba1BfalSphl(GCATG/C)NiaIII,Nsp1NiaIIIStylAvrilAvrII,Bfa1,BsaJ1,Sty1(C/CTAGG)Nhe1.Spe1,Xba1Bfal(C/CATGG)BspH1Nia川Neo1BsaJ1,Neo1,NiaIILSty1Taq1(T/CGA)Acc1(GT/CGAC),Cla1,BstB1Taq1Aci1,Acl1,BsaH1(GR/CGYC),HinP1I.HpaII,NarI-Tsp5091(/AATT)Apo1(R/AATTY),EcoR1,Mfe1Tsp5091Xbal(T/CTAGA)AvrII,Nhe1,Spe1,Sty1(C/CTAGG)BfalXhoimi1Ava1(C/TCGAG)Aval,Taq1,Xho1(C.TCGAGSallTaqlXma1Age1,BsaW1.BspE1,BsrF1,NgoMIV,SflrA1HpaII,Nci1,ScrFI(C/CCGGGAva1(C/CCGGG)Ava1,BsaJ1,HpaII.Nci1,ScrF1,Sma1.Xma1(A.'CGCGT)Asc1,BssHIIBstU1,Hha1(A/CATGT)BspH1,Neo1,PcilNiaIII(A/CGCGT)Miu1AfiIII,BstU1,Miu1Agel(A^CCGGT)Ava1(C/CCGGG),Xma1HpaII,Nci1,ScrFIBsaW1.BspE1BsaW1,HpaIIBsrFI(A.'CCGGT),SgrA1(CA/CCGGTG)Age1,BsaW1.BsrF1,HpaIINgoMIVBsrF1,HpaIIApa1(GGGCC/C)BanII(GGGCC/C),Bsp12861(GGGCC/C)Apa1,BanII,Bsp1201,Bsp1286I,HaeIII,NiaIV,Sau96IApaLI(G/TGCAC)Sfc1(C/TGCAG)BsglApo1(A^AATTY)EcoR1ApoI,Tsp509I(G/AATTY)EcoRIApoI,EcoRI,Tsp5091(R/AATTY)Mfe1,Tsp5091Tsp5O91Asc1(GG/CGCGCC)AfiIII(A/CGCGT),Miu1BstU1,Hha1BssHIIBssHII.BstU1,Cac81,Hha1Ase1(AT/TAAT)Bfa1.Csp61,Nde1-Mse1Mse1AsiSI(GCGAT/CGC)BsiE1(CGAT/CG)DpnII,Pvu1PadMselPvu1DpnII,Pvu1Ava1(C.'CCGGG)Age1,BsaW1,BspE1,BsrF1(R/CCGGY),NgoMIV,SgrA1(CR/CCGGYG)HpaII,Nci1,ScrFI(C.G-CGAG)XholAva1,Taq1.Xho1(C.nrCGAG)Sal1Taq1(C/CCGGG)Xma1Ava1,BsaJ1,HpaILNci1,ScrF1,Sma1AvaII(G/GWCC)PpuM1(RG/GACCY)AvaII,NiaIV,Sau961RsrllAvaII.Sau961PpuM1(RG/GTCCY)AvaII.BsmF1,NiaIV.Sau961AvrII(C/CTAGG)Nhe1,Spe1,Xba1BfalSty1(C/CTAGG)AvrII,Bfa1,BsaJ1,Sty1BamH1(G/GATCC)BelLDpnIIAlw1,DpnIIBglII,BstY1(R/GATCY)Alw1,BstY1,DpnIIBstY1(G/GATCC)Alw1,BamH1,BstYI,DpnII,NiaIVBan1(G/GTACC)ACC65IAcc651.Ban1,Kpn1,NiaIV.Rsa1(G/GCGCC)Kas1Ban1,BsaH1,HaeII,Hha1,Kas1,Nar1,NiaIV(G/GTACC)BsiW1,BsrG1Rsa1BanII(GGGCC/C)Apa1,Bsp1286I(GGGCC/C)Apa1,BanII,Bsp1286I,HaeIII,NiaIV,Sau961(GAGCT/C)Bsp12861(GAGCT/C),Sac1Alu1,BanII,BsiHKA1,Bsp1286I,Sac1Bell(T/GATCA)BamH1,BstY1(R/GATCY)Alw1,DpnIIBglII,MboIIDpnIIBfal(C/TAG)Ase1,Csp61,Mse1.Nde1-BglII(A.'GATCT)BamH1.BstY1(R/GATCY)Alw1,BstY1,DpnIIBel1,DpnIIDpnIIBsaHI(GR/CGYC)Acc1(GT/CGAC),Cla1,BstB1,Taq1—(GA-CGYC)Aci1,HinPlIHga1(GG/CGYC)AciI,HinPlIHhal(GG/CGYC)HpaIIAcil(G^CGYC)NarIBsaH1,Hga1(GG/CGYC)NariBan1,BsaH1,HaeII,Hha1,Nari,NiaIVBsaWI(W/CCGGW)Age1,BsrF1(R'CCGGY),SgrA1(CR/CCGGYG)Age1,BsaW1,BsrF1,HpaIIAva1(C/CCGGG),Xma1HpaII.Nci1,ScrFIBspE1BsaW1.BspE1,HpaIIBsrF1(R/CCGGY),NgoMIVBsrFLHpaIINgoMIVHpaIIBsiEI(CGAT/CG)PacIMsel(CGAT/CG)PvuIBsiE1,DpnII,Pvu1(CGGC/CG)SacIIAcilBsiHKA1(GTGCA/C)Bsp1286I(GTGCA'C)BsiHKAl,Bsp12861Bsp1286I(GAGCA/C)BsiHKA1,Bsp12861Bsp12861(GAGCT/C),Sac1Alu1,BanII,BsiHKA1,Bsp1286I,SacINsi1-Pstl,Sbf1BsglBsiWI(C/GTACG)Acc651,Ban1(G/GTACC),BsrG1RsaIBsp1286I(GGGCC/C)Apa1,BanII(GGGCC/C)ApaI,BanII,Bsp12861,HaeIII,NiaIV,Sau961(GTGCA/C)BsiHKA1ApaLI,BsiHKAl,Bsp1286l(GGGCC/C)BanII(GGGCC/C)BanII,Bsp1286I(GAGCT/C)BanII(GAGCT/C),BsiHKA1,Sac1AluI.BanII,BsiHKAI,Bspl286I,SacI(GWGCW/C)BsiHKA1BsiHKAI,Bsp1286l(GTGCA/C)Nsi1-(GTGCA/C)Pstl,Sbf1BsqIBspEIAge1,BsaW1,BsrF1(R/CCGGY),SgrA1(CR/CCGGYG)BsaWI,HpaII(T/CCGGA)Ava1(C/CCGGG),Xma1HpaII,Nci1,ScrFIBsaWIBsaW1,BspE1,HpaIIBsrF1(R/CCGGY),NgoMIVHpaIIBspHI(T/CATGA)AfiIII(A/CATGT),Neo1,Sly1(C/CATGG)NiaIIIBsrFI(A-'CCGGY)Age1,BsaW1Age1,BsaW1,BsrF1,HpaII(G/CCGGY)Age1,BsaWLNgoMIVBsrFLHpaII(R/CCGGY)Ava1(C/CCGGG),Xma1HpaII,Nci1,ScrFI(RCCGGY)BsaW1,BspEIBsaWI,HpaII(R/CCGGY)BsaW1.BspE1HpaII(G/CCGGY)NgoMIVBsrF1.Cac81,HpaII,Nae1(CR/CCGGYG)SgrA1BsrF1.HpaIIBsrG1(T/GTACA)Acc651,Ban1(G/GTACC),BsiW1Rsa1BssHII(G^CGCGC)AfiIII(A/CGCGT),Miu1BstU1,Hha1Asc1BssHII.BstU1,Cac81,Hha1BslBI(TT/CGAA)Acc1(GT/CGAC),Cla1,Taq1Taq1Aci1.Acl1,BsaH1(GRQGYC),HinP1I,HpaII.Nari一BstYI(NGATCY)BamHI,BglIIAlw1,BstY1,DpnII(G/GATCY)BamH1Alw1,BamH1,BstY1,DpnII,NiaIV(R/GATCY)Bel1,DpnIIDpnII(G/GATCY)Bel1,DpnIIAlw1,DpnII(NGATCY)BglIIBglII.BstY1,DpnIIClal(AT/CGAT)Acc1(GT/CGAC),BstB1,Taq1Taq1AciLAcl1,BsaH1(GFVCGYC),HinPlI,HpaII,Nari-DpnIVMboUSau3A1(/GATC)BamHLBstYI(R/GATCC)Alw1,DpnIIBelI,BglII,BstYI(R/GATCY)DpnIIEael(Y/GGCCR)PspOMIHaeIII.Sau961(C/GGCCR)Eag1BsiE1,Eae1,Eag1,HaeIII(T/GGCCR)Eag1Eae1,HaeIII(C/GGCCR)NotlAci1,BsiE1,Eae1,Eag1,Fnu4H1,HaeIII(T/GGCCR)NotlAci1,Eae1,Fnu4H1,HaeIIIEagl(C/GGCCG)PspOM1HaeIII.Sau961Eae1(Y/GGCCR)Eae1,HaeIIIEae1(C/GGCCG)BsiE1,Eae1,Eag1,HaeIIINotlAci1,BsiE1,Eae1,Eag1,Fnu4H1,HaeIIIEcoR1(G/AATTC)Apo1(G/AATTC)Apo1,EcoR1,Tsp5091Apo1(R/AATTY)Apo1,Tsp5091Mfe1,Tsp5091Tsp509IHinP11@CGC)Acc1(GT/CGAC),Acl1,Cla1,BstB1,Taq1-Aci1,BsaH1(GR/CGCC),NariHhalBsaH1(GR/CGTC)HgaIHpaIIAciIHpaIL/Msp1(C/CGG)Acc1(GT/CGAC),Acl1,Cla1,BstB1,Taq1-AciLBsaH1(GR/CGCC),HinP11,Nar1AciIKasl(G/GCGCC)Ban1(G/GCGCC)BanI,BsaHI,HaeII,HhaI,KasI,Nar1,NiaIVMfel(C/AATTG)Apo1(R/ATTTY),EcoR1,Tsp5091Tsp5091MIul(A^CGCGT)AfiIII(A/CGCGT)AfiIII.BstU1,Miu1Asc1,BssHIIBstU1,Hha1Mse1(T/TAA)AselMse1Bfa1,Csp61,Nde1-Nari(GG/CGCC)Acc1(GT/CGAC),Acl1,Cla1,BstB1,Taq1-Aci1,HinP1IHhalBsaHI(GR/