苏木化学成分研究

苏木化学成分研究

supply是sharmace的第四面。（卢姆纳斯），它被用作一个分散性和低水平的非物质区，用于体外生物和非物质区的研究和反射性。2.1.通过外在ro严重结构来表达违反相关规则标准的行为MeltingpointsweremeasuredonanX-4digitalmeltingpointapparatusandthetemperaturewasuncorrected.TheopticalrotationsweremeasuredonaPerkinElmer243Bdigitalpolarimeter.2.2.u2004u2005SappanLignumwaspurchasedfromAnguoofHebeiProvinceandidentifiedbyProfessorPeng-FeiTu(SchoolofPharmaceuticalSciences,PekingUniversity).AvoucherspecimenwasdepositedintheherbariumofPekingUniversityModernResearchCenterforTraditionalChineseMedicines.2.3.silcagractsor-etoacfraceofficiencipa之间的匹配SappanLignum(21.5kg)wasextractedwith95%EtOHfor2h,andrepeatedthreetimes,afterwhichthesolventwasremovedunderreducedpressure.Theethanolicextractwassuspendedinwater,andwaspartitionedwithpetroleumether,EtOAc,andn-BuOHsuccessively.TheEtOAcfractionwassubjectedtosilicagelcolumnchromatography,andelutedwithpetroleumether–EtOAc(2:1–1:5)toyield8fractions.Compound14(20mg)wascrystallizedfromfraction1.Compound6(15mg)wascrystallizedasyellowneedleswithEtOAcfromfraction2.Compound5(34mg)wasobtainedbycolumnchromatographyonsilicagel(CHCl3.1.brazil1Redamorphouspowder(MeOH).EI-MS:calcdforC3.2.sap潘眼2Whitepowder(MeOH).[a]3.3.e-3-3，4-dihydrol-4-i-7-hydrol-4-13Yellowneedles(MeOH),mp220–221°C.EI-MScalcdforC3.4.3-deoxisappan便于访问4Whitepowder(MeOH).EI-MS:calcdforC3.5.brazilidea5Colorlessneedles(Acetone),mp251–252°C.ESI-MS:calcdforC3.6.calcdf-msmeoh,mp3Yellowneedles(EtOAc),mp210–212°C.EI-MS:calcdforCYellowneedles(MeOH),mp310–312°C.EI-MScalcdforC3.8.rhaftin8Yellowneedles(MeOH),mp288–289°C.EI-MScalcdforC3.9.sapnachalcone9Yellowneedles(MeOH),mp198–199°C.ESI-MScalcdforC3.10.酶活剂Yellowneedles(MeOH),mp210–212°C.EI-MS:calcdforCYellowneedles(MeOH),mp211–213°C.EI-MS:calcdforC3.12.calcdf-msWhiteamorphouspower(MeOH).EI-MS:calcdforCWhiteamorphouspowder(MeOH).EI-MS:calcdforC3.14.itgatchiectin-sitoritysitorityColorlessneedles(petroleumether-EtOAc),mp137–138°C.Itwasidentifiedasβ-sitosterolbycomparisionoftheTLCbehaviorandthemeltingpointwiththeauthenticcompound.4.日homoisoflavanoids,2,3&4,3,4,4,4,4,4,4,4,4,3,4,4,4,3,4,4,3,4,4,4,4,3,4,4,4,4,4,3,4,3,4,4,4,4,3,4,3,4,4,3,4,3,4,3,4,4,4,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,4,3,FourteencompoundswereisolatedfromSappanLignum,includingonexanthone(6),onecoumarin(13),threechalcones(9,10and11),twoflavones(7and8),threehomoisoflavonoids(2,3and4),andbrazilin.Amongthem,compounds12and13wereidentifiedastwonewnaturalcompounds.Euxanthone(6)wasthefirstxanthoneisolatedfromthegenusofCaesalpinia.XanthoneswerefoundmainlyinGuttiferae,Gentianaceae,MaraceaeandPolygalaceae,butfewinLeguminosae.InLeguminosae,xanthonessuchasmangiferin,isomangiferinandcassiaxanth