支原体污染的预防

BIOLOGICALINDUSTRIESDOYOUSUSPECTMYCOPLASMA?

Prevention,DetectionandTreatmentofMycoplasmaContamination支原体污染的预防，检测与治疗第一页，共二十四页。BiologicalIndustriesoffersyou:

Treatment治疗Prevention预防Detection检测

MYCOPLASMA支原体第二页，共二十四页。MYCOPLASMADETECTION

支原体检测第三页，共二十四页。EZ-PCRMycoplasmaTestKitCat.#20-700-10(10assays)Cat.#20-700-20(20assays)ReadytousePCRmixforthedetectionofmycoplasmaincellculture即用型细胞培养中支原体检测PCR试剂盒第四页，共二十四页。EZ-PCRMycoplasmaTestKitisdesignedtodetectthepresenceofmycoplasmacontaminatingbiologicalmaterials,suchasculturedcells.EZ-PCR支原体检测试剂盒用来检测生物材料，如培养的细胞中支原体的存在。Mycoplasmadetectionbythedirectcultureprocedureistime-consumingandsomemycoplasmaspeciesaredifficulttocultivate.直接培养方法检测耗时且有的种类支原体很难培养。WithPCRtesting,resultsareobtainedwithinafewhours,sincethepresenceofcontaminantmycoplasmacanbeeasilydetectedsimplybyverifyingthebandsofamplifiedDNAfragmentsinelectrophoresis.用PCR检测，几小时可出结果。支原体DNA片段可通过电泳条带确认。第五页，共二十四页。第六页，共二十四页。Theprimersetallowsdetectionofvariousmycoplasmaspecies,

withhighsensitivityandspecificity:系列引物可以高特异性和高灵敏度地检测很多种支原体M.hyorhinisM.argininiM.bovisM.hominisM.pulmonisM.arthritidisM.salivariumM.oraleM.fermentansM.pirumM.pneumoniaeM.capricolumAcholeplasmaSpiroplasma第七页，共二十四页。TREATMENTOFMYCOPLASMA-INFECTEDCELLSWITHANTIBIOTICS

用抗生素治疗支原体污染的细胞第八页，共二十四页。Thecontaminationofcellswithmycoplasmaisaverycommonproblem,eventhoughitoftengoesunnoticedsincenocloudinessappearsinthecellculture.

支原体污染细胞培养是很常见的问题，常常因其不造成混浊而不被注意到。

Sincemycoplasma-infectedcellscannotalwaysbediscarded,manycomplicatedmethodshavebeensuggestedfortheeliminationofthemycoplasma.

支原体污染的细胞有时不能废弃，有许多复杂的方法可以用来去除污染。

第九页，共二十四页。BiologicalIndustriesisnowofferingacombinationofantibiotics,whichhavebeenshowntobeeffectiveintheeliminationofmycoplasmaspeciesthataccountfor90%ofthecontaminationfoundincellcultures.

BiologicalIndustries的抗生素系列产品能有效杀灭细胞培养中的90%以上的支原体BIOMYC-3(cat.#03-038-1)BIOMYC-1(cat.#03-036-1)&BIOMYC-2(cat.#03-037-1)第十页，共二十四页。BIOMYC-1isbasedontheantibiotictiamutin,whichisproducedbythefunguspleurotusmutilus.成分为泰妙菌素.

BIOMYC-2isbasedonminocycline,whichisatetracyclinederivative.二甲胺四环素，四环素衍生物.Thesetwoantibioticsolutionsaregenerallyusedsequentiallyincombination.以上抗生素溶液一般按顺序联合使用.

BIOMYC-1

&

BIOMYC-2

第十一页，共二十四页。BIOMYC-3BIOMYC-3isbasedontheciprofloxacinantibiotic,whichisamemberofthefluoroquinolonegroup.ManymycoplasmaspecieshavebeenfoundtobesensitivetoBIOMYC-3.BIOMYC-3成分为氟喹酮类的环丙沙星，多种支原体对其敏感.第十二页，共二十四页。PREVENTIONPHARMACIDAL(cat.#IC-110100)Disinfectantsolutionforincubatorsandsterilework-benches

incellculture-andmolecularbiologylaboratories用于消毒容器的表面

AQUAGUARD-1(cat.#01-867-1)Disinfectingof

CO2incubatorwaterbaths二氧化碳培养箱水槽专业抗支原体污染试剂

AQUAGUARD-2(cat.#01-916-1E)Disinfectingof

ordinaryWaterbaths一般培养箱水槽抗支原体处理试剂

第十三页，共二十四页。PHARMACIDALTheproblemofcontaminationinincubatorsand/orsterilework-benchesisoftenaseriousone,leadingtoextensivedamage.Non-toxicandbiodegradable,

andthereforecanbeusedforsprayingincubatorswhilethecellsremaininsidePreventscontaminationwithandgrowthoffungi(andspores),bacteria(andspores)mycoplasmaandviruses(includingHIVandHepatitisB)Activeingredients:QuarternaryBenzylammoniumcompounds.Thesolutiondoesnotcontainmercury,formaldehyde,phenoloralcohol.培养箱，无菌台的污染常导致更广泛的破坏。无毒和可降解性，可喷洒正进行细胞培养的培养箱。防止真菌（芽孢），细菌（芽孢），支原体和病毒（HIV&HBV）污染。活性成分为四苯甲基化合物,不含汞，甲醛，苯酚第十四页，共二十四页。AQUAGUARD-1

Fordisinfecting

CO2incubatorwaterbathsThewaterrequiredtocreatethehumidityisasourceofcontaminationwhichdispersesintheincubator.InordertodisinfectthewaterwerecommendAquaguard-1Solution,whichcontainsadisinfectantthatdoesnotcausedamagetothestainlesssteeltray,isnon-toxic,non-volatile,andextremelyeffective.Thewatershouldbereplacedwithsterilewatereverytwotofourweeks,adding50mlofAquaguard-1per5litersofwater.保持湿度的培养箱中的水是污染来源之一。消毒培养箱中的水，建议使用Aquaguard-1溶液，它不会腐蚀不锈钢盘，无毒，不挥发且极有效。水应每两到四周更换一次，每5升水加入50mlAquaguard-1溶液。第十五页，共二十四页。AQUAGUARD-2

Forpreventionofmicrobialgrowthinordinarywater

baths

用于防止普通水浴槽的微生物生长Aquaguard-2isintendedfordisinfectingvariouskindsofwaterbathsfrombacteriaandfungi.Aquaguard-2

用来消毒各种水浴箱Itisrecommendedtouse2mlofAquaguard-2foreachliterofwaterinthebath,andtorepeattheprocedureevery1-2weeks.After4-6weeks,thebathshouldbeemptiedandrefilledwithwatercontainingAquaguard-2.使用浓度为2ml/L,每1－2周重复一次，4－6周后彻底更换含Aquaguard-2的水TheactiveingredientinAquaguard-2issafetohumansanddoesnotcauseanyirritatingeffectstotheskinwhenusedintherecommendedconcentration.Aquaguard-2isbiodegradable.Aquaguard-2的有效成分在推荐使用浓度时对人体安全，且可降解。第十六页，共二十四页。MYCOPLASMAFAQ’s

常见问题第十七页，共二十四页。Whyisroutinemycoplasmatestingimportant?

为什么定期支原体检测很重要？Thecellfunctionsofmycoplasma-infectedcellsareaffected.Sinceitisdifficulttodetectinfectionreliablywithothermethods,itispossiblethatresultsofexperimentsarebeingmisinterpreted.支原体可改变细胞功能。用其他方法很难检测出感染，进而导致实验结果被曲解。第十八页，共二十四页。DoIhavetoculturemycellsintheabsenceofantibioticsbeforetesting?

检测前细胞培养中不能使用抗生素吗？Ifitisnotanurgentcase,itisrecommendedtoculturewithoutantibioticsforseveraldays,inordertomaximizethestrengthofthesignal,thatisobservedinPCR.建议在没有抗生素的情况下培养几天，以便最大化PCR观测信号。第十九页，共二十四页。CanItestcellsimmediatelyaftertheyhavebeenremovedfromliquidnitrogen?

细胞从液氮中取出后能够立即检测吗？Itisrecommendedtoletthemfirstgrowuntil90-100%confluencewithoutantibiotics.建议在无抗生素条件下生长至90－100％融合后。第二十页，共二十四页。HowfrequentlyshouldItestmycells?

检测频率？WhenevernewcellsenterthelabBeforeeachliquidnitrogenstorageRoutinelyevery2-3monthsWeeklyaftercontaminationhasbeenfoundinthelaboratoryWhenyoubelievethatcellscharacteristicshavebeenmodified新细胞进入实验室液氮保存前定期每2－3个月发现污染后每周发现细胞特性发生改变第二十一页，共二十四页。Whichantibiotic(s)shouldIusetotreatinfectedcells?

抗生素的选择Biomyc-1+Biomyc-2sequentially,orBiomyc-3aloneBiomyc-1+Biomyc-2按顺序联合使用，Biomyc-3单独使用Biomyc-1,Biomyc