2023学年完整公开课版Generalidentificationtesto

PharmaceuticalanalysisGeneralidentificationtestofmedicines(training)Generalidentificationtestofmedicines(training)PharmaceuticalidentificationConcept:Ananalyticalmethodforjudgingtheauthenticityofmedicinesbychemical,physicochemicalandbiologicalmethodsaccordingtocomposition,structureandphysicalandchemicalpropertiesofmedicines.Itismainlyusedtojudgetheauthenticityofmedicines,andsometimesusedtojudgethepurityofmedicines.PharmaceuticalidentificationContentGeneralidentificationtest:DistinguishdifferenttypesofmedicinesSpecificidentificationtest:DistinguishoneofthesamemedicinesMethodsChemicalidentificationSpectrophotometryChromatographyMedicineIdentificationKeyanddifficultpointsMasterIdentificationmethodsandprinciplesofseveralcommonchemicalmedicines.FamiliarOperationofseveralcommonchemicalmedicineidentificationtestsUnderstandAbilitytocorrectlyjudgeresults.Contents1.Identificationofaspirintablets2.Identificationofprocainehydrochlorideinjection3.IdentificationofofvitaminCtablet4.IdentificationofofvitaminB1tablet5.IdentificationofstreptomycinsulfateinjectionSectionIIdentificationofaspirintablet1.Identificationofaspirintablets(1)Structure(2)Property Thisproductiswhitetabletandeasytodeterioratewhenexposedtomoisture.(2)Identificationmethod Chemicalidentification

andhighperformanceliquidchromatographyCategorizedintosalicylicacids1.Identificationofaspirintablets(4)ExperimentalprincipleHydrolysis1)Ferrictrichloridereaction1.Identificationofaspirintablets(5)OperationstepsTakeappropriateamountoffinepowerofthisproductAdd10mlwaterBoilitCoolitAdd1dropofferricchloridetestsolutionPurpleblueEquivalentto0.1gaspirinSectionIIIdentificationofprocainehydrochlorideinjection2.Identificationofprocainehydrochlorideinjection(1)Structure(2)Property Thisproductiscolorlessclearliquid.(3)Identificationmethod Chemicalidentificationandultraviolet-visiblespectrophotometryCharacteristics:benzocaine,structureofprimaryaromaticamine,tertiaryaminenitrogen,hydrochloride2.Identificationofprocainehydrochlorideinjection(4)Experimentalprinciple1)ReactionofaromaticprimaryamineDiazoniumsaltScarletprecipitateDiazoniumsaltProcainehydrochloride2.Identificationofprocainehydrochlorideinjection(4)Experimentalprinciple2)IdentificationreactionofgeneratingchlorideWhiteprecipitatePrecipitatesolublePrecipitateregenerated2.Identificationofprocainehydrochlorideinjection(5)Operationsteps

1)Reactionofaromaticprimaryamine(2)IdentificationreactionofgeneratingchlorideAddHClAdd0.1mol/LNaNO2solution

Addbasicβ-naphtholreagentScarletprecipitateTake50mgtestproductAddHNO3foracidificationAddsilvernitrateWhiteflocculentprecipitateAddammoniasolutionPrecipitatesolubleWhiteprecipitateTestsolutionAddHNO3foracidificationSectionIIIIdentificationofvitaminCtablet3.IdentificationofvitaminCtablet(1)Structure(2)Property Thisproductiswhitetablet.(3)Identificationmethod

ChemicalidentificationandthinlayerchromatographyWithdienolstructureandreducibility3.IdentificationofvitaminCtablet(3)Experimentalprinciple1)Reactwithsilvernitratetestsolution(2)ReactwithsodiumdichlorophenolindophenolatetestsolutionAcid,rosered;alkali,blueColorlessphenolimineDehydrovitaminCBlacksilverprecipitation3.IdentificationofvitaminCtablet(4)Operationsteps(1)Pre-treatmentofvitaminCFiltrate0.5mlsilvernitratetestsolution1-2dropsofsodiumdichlorophenolindophenolatetestsolutionBlacksilverprecipitationColordisappears(2)ChemicalidentificationTakeappropriateamountofvitaminCtablets10mlwaterTakeappropriateamountoffinepowderequivalentto0.2gvitaminCFilteredFiltratePorphyrizeShakeitSectionIVIdentificationofvitaminB1tablet4.IdentificationofofvitaminB1tablet(1)Structure(2)Property

Thisproductiswhitetablet.(3)Identificationmethod

ChemicalidentificationandinfraredspectrophotometryCharacteristics:Quaternaryaminealkaliformedbyconnectionofaminopyrimidineandthiazoleringthroughmethylene;Hydrochloride4.IdentificationofofvitaminB1tablet(4)Experimentalprinciple1)ThiochromereactionSolubleinN-butylalcoholBluefluorescence2)Identificationreactionofgeneratingchloride(sameasprocainehydrochloride)SpecificityreactionofvitaminB1Thiochromewithfluorescence4.IdentificationofofvitaminB1tablet(5)Operationsteps1)Pre-treatmentofvitaminB1tabletTakeappropriateamountofvitaminB1tabletsTakeappropriateamountoffinepowerFilterEvaporatetodrynessFiltrateObtainresiduesGrindAddwaterfordissolutionAddacidwithfluorescencedisappearing0.5mlpotassiumferricyanide+5mlN-butylalcoholAddsodiumhydroxidefordissolutionObtainappropriateamountofresiduesShakeitBluefluorescenceinalcohollayerAddalkali,withfluorescencereappearing2)SpecificityidentificationSectionVIdentificationofstreptomycinsulfate5.Identificationofstreptomycinsulfate(1)Structure(2)Property

Thisproductiswhiteoroff-whitepowder.(3)IdentificationmethodChemicalidentificationandinfraredspectrophotometryStreptidineStreptoseN-methylglucose5.Identificationofstreptomycinsulfate(4)Experimentalprinciple1)SakaguchireactionOrange-redcompound2)MaltolreactionMaltolPurple-redSpecificreactionofstreptomycinhydrolysate-streptidineStreptomycinStreptomycin8-hydroxyquinolineethanolsolution(ornaphthol)5.Identificationofstreptomycinsulfate(4)Experimentalprinciple3)Identificationreactionofsulfateinaqueoussolutionofthisproduct2)Reactionwithleadacetate:DissolvePrecipitatedinhydrochloricacidornitricacidwithoutdissolution1)Reactionwithbariumchloride:5.Identificationofstreptomycinsulfate(5)Operationsteps1)Sakaguchireaction2)MaltolreactionAdd4mlwatertodissolveitNaOH2.5ml,1ml0.1%8-hydroxyquinoline0.5mgtestproductAdd3dropsofNaBrOCoolitOrange-red