Polyinosinic-polycytidylic-acid-potassium-Poly-I-C-potassium-DataSheet-生命科学试剂-MCE

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemEPolyinosinic-polycytidylicacidpotassiumCat.No.:HY-134958CASNo.:31852-29-6Synonyms:Poly(I:C)potassium分⼦式:(C₁₀H₁₃N₄O₈P)x.(C₉H₁₄N₃O₈P)x.xK作⽤靶点:Toll-likeReceptor(TLR);Apoptosis作⽤通路:Immunology/Inflammation;Apoptosis储存⽅式:PleasestoretheproductundertherecommendedconditionsintheCertificateofAnalysis.BIOLOGICALACTIVITY⽣物活性Polyinosinic-polycytidylicacidpotassium(Poly(I:C)potassium)双链RNA的合成类似物，⼀种TLR3和视酸诱导型因I受体(RIG-I和MDA5)的激动剂。Polyinosinic-polycytidylicacidpotassium可以⽤作疫苗佐剂，以增强先天性和适应性免疫反应，并改变肿瘤的微环境，还可以直接触发癌细胞发⽣凋亡(Apoptosis)。IC50&TargetTLR3TLR3体外研究Polyinosinic-polycytidylicacidpotassium(20ng/mL;24hours;WM793,WM278,WM239A,WM9and1205Lucells)inhibitscellgrowth[1].Polyinosinic-polycytidylicacidpotassium(200ng/mL;24hours;1205Lucells)inducesapoptosisin1205Lucells[1].Polyinosinic-polycytidylicacidpotassium(3ng/mL;24hours;1205Lucells)inducesIFN-βexpressioninmelanomacells.SilencingofRIG-IandMDA-5confirmedthatinductionofIFN-βbyPolyinosinic-polycytidylicacidrequiredRIG-IandMDA-5,respectively,andthatrequiredIPS-1[1].Polyinosinic-polycytidylicacidpotassiumispreparedforinjectionbyresuspendinginsterilesaline,heatingto50°Cataconcentrationof2mg/mltoensurecompletesolubilisationandthenallowingtocoolnaturallytoroomtemperaturetoensureproperannealingofdouble-strandedRNA.PolyI:Cisstoredat-20°Cuntiluse[3].CellViabilityAssay[1]CellLine:WM793,WM278,WM239A,WM9and1205Lucells1/3MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemEConcentration:20ng/mLIncubationTime:24hoursResult:Stronglyreducedviabilityfrom100%incontrolsto20%–50%within24hours.ApoptosisAnalysis[1]CellLine:1205LucellsConcentration:200ng/mLIncubationTime:24hoursResult:Inducedapoptosisin1205Lucells.RT-PCR[1]CellLine:WM793,WM278,WM239A,WM9and1205LucellsConcentration:3ng/mLIncubationTime:24hoursResult:InducedIFN-βexpressioninmelanomacells.WesternBlotAnalysis[1]CellLine:1205LucellsConcentration:5ng/mLIncubationTime:24hoursResult:Revealedactivesubunitsofcaspase-9andcaspase-8inmelanomacells.体内研究Polyinosinic-polycytidylicacidpotassium(50µg;i.v.;ondays3,6,and9aftertumorinoculation)showsantitumoractivityinNOD/SCIDimmunodeficientmice[1].AnimalModel:NOD/SCIDimmunodeficientmice(1205Lucells)[1]Dosage:50µgAdministration:I.v.;ondays3,6,and9aftertumorinoculationResult:Reducedthesizeofmetastasesandthetotalamountoftumortissue,andthelevelofhumanDNAwas50%lowerinmice.2/3MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemE户使⽤本产品发表的科研⽂献•AdvFunctMater.29August2022.•ChemEngJ.2021,129392.•LiverInt.2022Oct17.•Phytomedicine.2021,153495.•MolTherOncolytics.25August2022.Seemorecustomervalidationsonwww.MedChemEREFERENCES[1].BeschR,etal.ProapoptoticsignalinginducedbyRIG-IandMDA-5resultsintypeIinterferon-independentapoptosisinhumanmelanomacells.JClinInvest.2009Aug;119(8):2399-411.[2].ChengYS,etal.Anticancerfunctionofpolyinosinic-polycytidylicacid.CancerBiolTher.2010Dec15;10(12):1219-23.[3].RobertField,etal.SystemicchallengewiththeTLR3agonistpolyI:CinducesamplifiedIFNalpha/betaandIL-1betaresponsesinthediseasedbrainandexacerbateschronicneurodegeneration.BrainBehavImmun.2010Aug;24(6):996-1007.McePdfHeightCaution:Producthas