分子生物学Molecular Biology智慧树知到答案章节测试2023年山东第一医科大学

第一章测试TheNucleicacidwillgetmaximumUVabsorbanceatwavelength_____（）

A:270nm

B:260nm

C:280nm

D:230nm

答案:BThecommoncomponentcomposedofDNAandRNAis_____（）

A:Thymine

B:D-ribose

C:Guanine

D:D-2-deoxyribose

答案:CThesecondarystructureofDNAis_____（）

A:β-turn

B:β-sheet

C:α-helix

D:doublehelix

答案:DThecorrespondinganticodontocodonACGis___（）

A:GCA

B:CGU

C:UGC

D:CGT

答案:BSinglestrandDNA5’-CGGTA-3’canannealwiththefollowRNA_____（）

A:5’-TAGGC-3’

B:5’-UACCG-3’

C:5’-GCCAT-3’

D:5’-GCCAU-3’

答案:BmRNAisusedas_____intranslation.（）

A:template

B:None

C:adaptor

D:machinery

答案:AAmountofTinRNAis0.25mol，soamountofAis_____.（）

A:1.00mol

B:0.5mol

C:0.25mol

D:0.75mol

答案:CtRNAineukaryoticcellcontains_____（）

A:5.8sRNA

B:5sRNA

C:18sRNA

D:28sRNA

答案:ABCDThebaseinDNA_____（）

A:C

B:T

C:A

D:G

答案:ABCDStem-loopisatypeofsecondarystructurenotusuallyfoundintRNA.（）

A:错

B:对

答案:BAllgenomesarecomposedofRNA.（）

A:错

B:对

答案:A第二章测试SequencesthatareremovedfromtheprimaryRNAtranscriptineukaryotesarecalled________.（）

A:introns

B:exons

C:mRNA

D:operons

答案:AWhichcharacterisnottrueforeukaryoticgenes?（）

A:Alltheprotein-codinggenesinhighereukaryoteshaveintrons.

B:Thereisahighlyconservedsequenceatthejunctionofexonandintron.

C:GenesofrRNAandsometRNAhaveintronstoo.

D:Eukaryoticgenestructureisdiscontinuous

答案:AWhichisnotafeatureofenhancer？（）

A:Sometimesenhancersequencescanalsobelocatedwithinintrons.

B:Ageneoftenhasonlyoneenhancer.

C:Thedistancebetweenenhancersequencesandregulatedgenesvariesalot.

D:Itcanenhancethepromoterinanydirectionandatanylocation

答案:BTheregulatorsofeukaryoticgeneexpressionconsistof________.（）

A:promoter

B:enhancer

C:insulator

D:silencer

答案:BCDAllhousekeepinggeneshaveweakpromotorsandareregulatedtomaintaintheirgeneproductsatconstantlowlevelconcentrations.（）

A:对

B:错

答案:B第三章测试BasedonMeselson-Stahlexperiment,eachdaughterDNAmoleculeiscomposedof（）

A:twooldparentalstrands

B:twostrandswithbothnewandoldDNA

C:oneoldandonenewstrand

D:twonewdaughterstrands

答案:CWhichmechanismcanexplaintwostrandsaresynthesizedintheoppositedirection,inthereplicationfork?（）

A:semidiscontinuousreplication

B:semiconservativereplication

C:bidirectionalreplication

D:primingofDNAsynthesis

答案:AAlthoughmammaliangenomeishuge,cellscanreplicatethegenomeinjustafewminutes.Whichisthemostpossiblereason?（）

A:RNApolymerasescarryoutreplication

B:EukaryoticDNApolymerasesareextraordinarilyfasterthanprokaryotes

C:Theuniquecellcycle

D:Hundredsofreplicationforksworksimultaneously

答案:DWhichisTUREabouttheinitiationofprokaryoticDNAreplication?（）

A:DNApolymeraseIIIremovesprimer

B:TopoisomerasesynthesizesanRNAfragment

C:DNApolymerseIsynthesizesnewlysynthesizedstrand

D:DNAhelicaseunwindsDNAduplexatoriC

答案:DWhichis

FALSEabouttheterminationofprokaryoticDNAreplication?（）

A:DNAligasesealsallDNAnicks

B:Coordinatethesynthesisoflaggingandleadingstrands

C:DNApolymeraseIfillsgapsandleavesnicks

D:DNApolymeraseIremovesallRNAprimersandleavesgaps

答案:BWhichisFALSEaboutDNApolIIIinprokaryotes?（）

A:βclampsaidittofixDNAtemplates

B:ItisthekeyenzymeinDNAreplication

C:itcancatalyzetheformationof3’-5’phosphodiesterbond

D:α,δ,ξsubunitsformcoreenzyme

答案:DMitochondrialDNAreplicatesin（）

A:conservativereplication

B:reversetranscription

C:dispersivereplication

D:D-loopreplication

答案:DRNAprimerinDNAreplication（）

A:staysinthedaughterDNAduplex

B:isrequiredbyallOkazakifragments

C:provides3’-OHtosynthesizeDNA

D:activatesDNApolymerase

答案:BCDNAreplicationneeds（）

A:helicase

B:primase

C:topoisomerase

D:DNAligase

答案:ABCDWhatarethedifferencesofeukaryoticDNAreplicationfromprokaryotes?（）

A:thereplicationoriginofeukaryotesisshorterthanthatinprokaryotes

B:telomereandtelomeraseaidterminationofeukaryotes

C:eukaryoticDNAisreplicatedinSphaseofcellcycle

D:eukaryoticDNAcontainsmultipleorigins

答案:ABCDTheOkazakifragmentofeukaryotesislongerthanthatofprokaryotes.（）

A:对

B:错

答案:BReversetranscriptaseexertsthefunctionsofRNAdependentDNApolymeraseandDNAdependentDNApolymerase.（）

A:错

B:对

答案:B第四章测试WhichofthefollowingDNAdamagesbelongstoexogenousDNAdamage（）.

A:Ionizingradiation

B:InaccuracyinDNAreplication

C:EndogenousOxidativeDNAdamage

D:InstabilityofDNAstructure

答案:AWhichtypeofDNApointmutationswillnotleadtothechangeinaminoacid（）.

A:Silentmutation

B:Nonsensemutation

C:Noneofthem

D:Missensemutation

答案:Awhichbasesubstitutionbelowbelongsto“Transition”pointmutation.（）

A:G→T

B:A→T

C:C→T

D:A→C

答案:CWhichiswrongbelowasdescribedfordirectlyundoingDNAdamagesrepair.（）

答案:DInwhichprocessGG-NERandTC-NERshowdifferent（）

A:DNAlesionrecognition

B:DNAstrandunwinding

C:DNAStrandexcision

D:Nucleotidesresynthesis

答案:AThedeficiencyofNERrepairproteinsissusceptibleto_disease.（）

A:Xerodermapigmentosum

B:SickleHbC

C:Lynchsyndrome

D:Chronicmyelogenousleukemia

答案:ADuringmismatchrepairinprokaryotes,thenewlysynthesizedDNAisrecognizedbythelackof()modification（）

A:Methylation

B:Phosphorylation

C:Acetylation

D:Deamination

答案:ADuringdoublestrandedDNAbreakrepair,NHEJshowsmoreprecisecomparedtoHR.（）

A:错

B:对

答案:ADNAdamageisthemaincausefortheincreasedfrequencyofgenemutation.（）

A:错

B:对

答案:BBaseexcisionrepairisoneofDNArepairpathwaystocorrectslargebaselesions.（）

A:对

B:错

答案:B第五章测试WhichcomponentoftheprokaryoteRNApolymeraseholoenzymeisinvolvedinrecognizingpromotersspecifictocertaintypesofgenes?（）

A:β

B:β’

C:σ

D:α

答案:CWhichRNApolymerasetranscribesthegeneofinsulin?（）

A:RNApolymeraseIII

B:RNApolymeraseII

C:RNApolymeraseI

D:RNApolymeraseIV

答案:BAlltheRNAsbelowaretranscribedbyRNApolIexcept（）

A:5.8SrRNA

B:28SrRNA

C:23SrRNA

D:18SrRNA

答案:CWhichofthefollowingstepsoccursfirstinprokayotictranscription?（）

A:Rhoproteinbinding

B:formationofanopenpromotercomplex

C:formationofaclosedpromotercomplex

D:promoterclearance

答案:CTheRhoproteinisinvolvedinthe_____stageoftranscription.（）

A:termination

B:initiation

C:elongation

D:post-transcriptionalprocessing

答案:AInprokaryotes,RNApolymerasecanbindupstreamsequencesofpromotersdirectly.Eukaryotesneedsomeproteinstofacilitatethebinding.Whatarethenamesoftheseproteins?（）

A:TATAboxproteins

B:Exons

C:Introns

D:Transcriptionfactors

答案:DWhichtranscriptionfactorcanrecognizeandbindtoTATAbox?（）

A:TFIIB

B:TFIIH

C:TFIIE

D:TFIID

答案:DAmutationtothissequenceineukaryoticmRNAwillaffecttheprocessbywhichthe3’-endpoly-AtailisaddedtothemRNA.（）

A:AAUAAA

B:TATAAA

C:TTGACA

D:CAAT

答案:AThefunctionsof5’CapofeukaryoticmRNAinclude（）

A:accelerationoftheaddingofpolyAtail.

B:transportofthemRNAfromnucleustocytosol;

C:enhancementofthemRNA’stranslatability;

D:protectionofthemRNAfromdegradation;

答案:BCDCis-actingelementsandtrans-actingfactorscontroltranscription.Thecis-actingelementsincludetheTATAbox,enhancersandotherelementsetal.（）

A:错

B:对

答案:BRNAPolIIistheenzymesynthesizinghnRNAineukaryotes.Itisnotsusceptibletoamanitin.（）

A:对

B:错

答案:BEukaryotictranscriptionsisaccompaniedbytranslation.（）

A:对

B:错

答案:B第七章测试WhichisNOTafinalproductofgeneexpression?（）

A:tRNA

B:rRNA

C:mRNA

D:protein

答案:CIfageneisexpressedatrelativelyconstantrateinalmostallthecells,itiscalleda(an)（）

A:induciblegene

B:constantgene

C:housekeepinggene

D:repressiblegene

答案:CThemostefficientstepofgeneexpressionregulationis（）

A:activationofchromosomes

B:translationinitiation

C:transcriptioninitiation

D:transcriptiontermination

答案:CWhichoneisNOTacis-actingelement?（）

A:silencer

B:promoter

C:activatorprotein

D:enhancer

答案:CTheprokaryoticoperatorsequenceisusually（）.

A:locatedonthedownstreamregionofpromoter

B:boundbyCAP

C:locatedontheupstreamregionofpromoter

D:boundbyRNApolymerase

答案:CIntheregulationoflacoperon,whenthemediumcontainsbothglucoseandlactose,whichstatementistrue?（）

A:bothrepressorandCAPareactive

B:bothrepressorandCAPareinactive

C:repressorisactive;CAPisinactive

D:repressorisinactive;CAPisactive

答案:BInlacoperon,repressorproteincouldbindto()sequence.（）

A:lacI

B:operator

C:lacZ

D:promoter

答案:BIntheregulationoflacoperon,whenthemediumcontainsonlyglucose,whichstatementistrue?（）

A:concentrationofcAMPishigh,CAPisactive

B:concentrationofcAMPislow,CAPisinactive

C:concentrationofcAMPislow,CAPisactive

D:concentrationofcAMPishigh,CAPisinactive

答案:BIfageneisexpressedonlyincertainorgansortissues,thisiscalled（）

A:temporalspecificityofgeneexpression

B:noneoftheabove

C:spatialspecificityofgeneexpression

D:stagespecificityofgeneexpression

答案:CWhichisNOTthebasalcis-actingelementsineukaryotes?（）

A:insulator

B:enhancer

C:promoter

D:operator

答案:DEnhancersmustlocateontheupstreamregionofpromoter.（）

A:错

B:对

答案:ATheactivechromatinusuallyshowslowlevelofmethylation.（）

A:错

B:对

答案:B第八章测试Whichstatementistrueaboutoncogenes?（）

A:Theirproductsinhibitcellproliferation

B:Hypermethylationhappensintheirpromoterregion

C:Oncogenesareonlypresentincancercells

D:Theyareusuallyactivatedincancerdevelopment

答案:DWhichoneofthefollowingisoncogene?（）

A:PTEN

B:myc

C:Rb

D:p53

答案:BThefollowingchangespromoteoncogeneactivation,except（）

A:Lossofheterozygosity

B:Amplification

C:Mutation

D:Translocation

答案:AThemechanismsoftumorsuppressorgeneinactivationinclude（）

A:Obtainingnewpromoterorenhancer

B:Mutation

C:hypermethylation

D:Lossofheterozygosity

答案:BCDInmostchronicmyelogenousleukemia,tumorigenesisiscausedbyoncogeneamplification（）

A:错

B:对

答案:A第九章测试ThebasicstepsofPCRreactionare:（）

A:denaturation,extension,annealing

B:annealing,extension,denaturation

C:denaturation,annealing,extension

D:annealing,denaturation,extension

答案:CThebasiccomponentsofthePCRsystemdoesnotinclude:（）

A:dNTP

B:thermalstableDNApolymerase

C:NTP

D:templateDNA

答案:CWhichtechnologycanbeusedtoidentifyDNA?（）

A:Southernblotting

B:Westernblotting

C:Northernblotting

D:affinitychromatography

答案:AWhicharerightaboutmolecularhybridization?（）

A:CanonlyhybridizationbetweenDNAandRNA

B:FromtheunderstandingofDNAdenaturationandrenaturationphenomenon

C:Hybridmoleculesmustbecompletelybasepairing

D:Thehybridmoleculesmustbesingle

答案:BDInthePCRreaction,thepurposeofannealingistomakethecombinationofprimerandtemplate.（）

A:错

B:对

答案:BProteinchiptechnologyhasthecharacteristicsoffastandhighthroughput,itcanbeusedtoanalyzethousandsofproteinsinthewholegenomelevelatthesametime.（）

A:错

B:对

答案:B第十章测试WhichofthefollowingstatementsisnottrueconcerningTypeIIrestrictionendonucleases?（）

A:Recognizepalindromesequence

B:Stickyendsorbluntendsareproducedafterdigestion

C:TheycancleavesingleDNA

D:CutDNAintofragmentsatrecognitionsite

答案:CWhichisnotnecessaryaspartofprokaryoticexpressionvector?（）

A:Promoter

B:PolyAsignal

C:Ribosomebindingsite

D:Selectablemarkers

答案:BBasedonthenamingrules,thefirstletterofarestrictionendonucleasemeans（）

A:Bacterialgenus

B:Identifiedorder

C:Bacterialstrain

D:Bacterialspecies

答案:AIsocaudarnerpresents（）

A:Variousrestrictionendonucleasesrecognizesamepalindrome,butcutatidenticalsite

B:Variousrestrictionendonucleasesrecognizedifferentpalindromes,butproducesamestickyends

C:Variousrestrictionendonucleasesrecognizesamepalindrome,butcutatdifferentsite

D:Variousrestrictionendonucleasesrecognizesamepalindromes,andproducesamestickyends

答案:BPlasmidsareusuallyintroducedintobacterialcellsby.（）

A:Transformation

B:Transfection

C:Infection

D:Transduction

答案:AT4DNAligaserequires（）

A:GTP

B:NAD+

C:AMP

D:ATP

答案:DIninsertionalinactivationscreening,thebacteriathataresensitivetotetracyclineandresistanttoampicillinshouldbetransformedwith（）

A:Self-cyclizedvectors

B:ForeignDNAo