双酶切反应通用缓冲液使用表

DoubleDigestion（双酶切反应）时UniversalBuffer（通用缓冲液）的使用表■说明使用二种酶同时进行DNA切断反应（DoubleDigestion）时，为了节省反应时间，通常希望在同一反应体系内进行。TaKaRa采用UniversalBuffer表示系统，并对每种酶表示了在各UniversalBuffer中的相对活性。尽管如此，在进行DoubleDigestion时，有时还会难以找到合适的UniversalBuffer。本表以在pUC系列载体的多克隆位点处的各限制酶为核心，显示了在DoubleDigestion可使用的最佳UniversalBuffer条件。在本表中，各UniversalBuffer之前表示的［数字刈是指各UniversalBuffer的反应体系中的最终浓度。TaKaRa销售产品中添附的UniversalBuffer全为10倍浓度的缓冲液。终浓度为0.5x时反应体系中的缓冲液则稀释至20倍，1x时稀释至10倍，2x时稀释至5倍进行使用。■注意◊1曲DNA中添加10U的限制酶，在50pl的反应体系中，37°C下反应1小时可以完全降解DNA。◊为防止Star活性的产生，请将反应体系中的甘油含量，尽量控制在10%以下。◊根据DNA的种类，各DNA的立体结构的差别，或当限制酶识别位点邻接时，有时会发生DoubleDigestion不能顺利进行的可能。EnzymeAcc]BamH1BgHICfaI5cdRIECORVHineElHindHIKpn1NeoiNde\AccE1xM0,5xK牝T1xM1*M0.5xK1XM1XMMM+BSAixrBjjtiH10.5XK1XK1XK1xK1XK1XK0,5XK1XK0.5XKIxK+BSAix<B单II1XT1XK1XH1xH1XH1xH2XK1xK1XT1XK+BSA1mHClal1xM1XK1XH1xM1XH1XH1XM1XM1xM1XK+0SA1xHEdoR11XMI一I欢孑1xH1XH1XH1XH1KM1XUt尺M1XK+0SA1XHECORV0.5XK1XK1xH1XH1XH1XHZXT1xK0.5"IxKtBSA1xHHine耻1xW0.5xK2XK1XM1xM2XT1XM1XM1XMlx娅SA1XTHfndl[JIxM1XK1xK1KM1xM1xK1xM1xMfKM1XK+0$AIxKKpn11XM0.5XK1xT1XMEM0.5XK1XM1XM1XL站WKtBSJ1】TNc-oIixLI+BSAIxKiBSAUK+BSAtxK+嘛1祁+B$AIxK^BSAIxM两IxKtBSA0,5x将啪AIxKtBSA1XK+BSANtfeI1XTIXK1XH1xH1XH1xH1XT1xK1XT1xK+0$A1XHMpH05XK+BSAOSxK+BSA，俱BEAIXH^BSA1XK+BSAIXH-^BSAO.5KK40SA顾ABSAO.SKK4BSAI5.6XK+SBAuh^psaPsn1KM1xK1XH1XH1xH1XH1xM1"1xMIxK+BSA1XHp他i0.5XKIXK1XK1x；K1XK1xK0.5XKIXK0,5XKIxK^SAIXK。甘£i1XM0.&XKO.$XK1xM1XM0.5XK1xM1xM1XL(J.5XK+BSAESail1.5XTL5XT1XH1XH1XH1XH1.5XK1：5XK1.5XT+B5A1.5XT+6SA1xHSms11MT+BSA皿JtT+W1x140UbSSA1XT40£AC.5XK4&SAIxT+BSAims口1xT+0$A1XT+BSAIxT+BSASpe[1xMIxKIxH1xMIxHixh1xM1xM1xMIxK+BSA1xHSpAl0.5xK1xK1XH1XH1XH1XH2XT1xK0.5XKIxK+BSA1XHXJt»3Eixyt0.5XK2XT1XM1xM2XT1XM1xMfXM1XLMSA1^T加11xMIxK1xH1xHImH1xH1xM1xM1小1xK1xH

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