ML-SA1-DataSheet-生命科学试剂-MedChemExpress

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemEML-SA1Cat.No.:HY-108462CASNo.:332382-54-4分⼦式:C₂₂H₂₂N₂O₃分⼦量:362.42作⽤靶点:TRPChannel作⽤通路:MembraneTransporter/IonChannel;NeuronalSignaling储存⽅式:Powder-20°C3years4°C2yearsInsolvent-80°C6months-20°C1month溶解性数据体外实验DMSO:20.83mg/mL(57.47mM;ultrasonicandwarmingandheatto65°C)MassSolvent1mg5mg10mgConcentration制备储备液1mM2.7592mL13.7961mL27.5923mL5mM0.5518mL2.7592mL5.5185mL10mM0.2759mL1.3796mL2.7592mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存⽅式和期限：-80°C,6months;-20°C,1month。-80°C储存时，请在6个⽉内使⽤，-20°C储存时，请在1个⽉内使⽤。体内实验请根据您的实验动物和给药⽅式选择适当的溶解⽅案。以下溶解⽅案都请先按照InVitro⽅式配制澄的储备液，再依次添加助溶剂：(为保证实验结果的可靠性，澄的储备液可以根据储存条件，适当保存；体内实验的⼯作液，建议您现⽤现配，当天使⽤；以下溶剂前显⽰的百分⽐指该溶剂在您配制终溶液中的体积占⽐；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的⽅式助溶)1.请依序添加每种溶剂：10%DMSO>>40%PEG300>>5%Tween-80>>45%salineSolubility:≥2.08mg/mL(5.74mM);Clearsolution1/2MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemE2.请依序添加每种溶剂：10%DMSO>>90%cornoilSolubility:≥2.08mg/mL(5.74mM);ClearsolutionBIOLOGICALACTIVITY⽣物活性ML-SA1作为⼀种选择性TRPML激动剂，通过促进溶酶体的酸化和蛋⽩酶活性，抑制登格病毒2(DENV2)和寨卡病毒(ZIKV)。ML-SA1抗DENV2RNA和ZIKVRNA的IC50值分别为8.3μM和52.99μM。ML-SA1诱发⾃噬。ML-SA1可⽤于⼴谱抗病毒研究[1]。IC50&TargetIC50:8.3μM(DENV2)[1].IC50:52.99μM(ZIKV)[1]体外研究ML-SA1(25μM;0~14hours;A549cells)possiblyaffectstheentryofDENV2intohostcells[1].ML-SA1(0~200μM;A549cells)showsthatthereisnocytotoxicitytothecelllineobserved,evenatconcentrationsupto200μM.ML-SA1(0~50μM;A549cells)significantlysuppressesDENV2attheRNAlevelsandtheIC50is8.93μM[1].ML-SA1resultsinadose-dependentdecreaseinZIKVinA549cellsatboththeRNAandproteinlevels,andtheIC50valueofML-SA1againstZIKVRNAis52.99μM.ML-SA1,asanactivatorofTRPMLs,appearstobeapotentinhibitorofDENV2andZIKVinvitro.ML-SA1promoteslysosomalacidificationandproteaseactivitytoinhibitviralinfection.ML-SA1caninduceautophagyinHuh7cellsorA549cells[1].WesternBlotAnalysis[1]CellLine:A549cellsConcentration:25μMIncubationTime:0~14hoursResult:PossiblyaffectedtheentryofDENV2intohostcells.户使⽤本产品发表的科研⽂献•Autophagy.2022Sep10;1-16.Seemorecustomervalidationsonwww.MedChemEREFERENCES[1].XiaZ,etal.ML-SA1,aselectiveTRPMLagonist,inhibitsDENV2andZIKVbypromotinglysosomalacidificationandproteaseactivity.AntiviralRes.2020;182:104922.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.