Adavivint-SM04690-DataSheet-生命科学试剂-MedChemExpress

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemEAdavivintCat.No.:HY-109049CASNo.:1467093-03-3Synonyms:SM04690;Lorecivivint分⼦式:C₂₉H₂₄FN₇O分⼦量:505.55作⽤靶点:Wnt作⽤通路:StemCell/Wnt储存⽅式:Powder-20°C3years4°C2yearsInsolvent-80°C6months-20°C1month溶解性数据体外实验DMSO:25mg/mL(49.45mM;Needultrasonicandwarming)H2O:<0.1mg/mL(insoluble)MassSolvent1mg5mg10mgConcentration制备储备液1mM1.9780mL9.8902mL19.7804mL5mM0.3956mL1.9780mL3.9561mL10mM0.1978mL0.9890mL1.9780mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存⽅式和期限：-80°C,6months;-20°C,1month。-80°C储存时，请在6个⽉内使⽤，-20°C储存时，请在1个⽉内使⽤。体内实验请根据您的实验动物和给药⽅式选择适当的溶解⽅案。以下溶解⽅案都请先按照InVitro⽅式配制澄的储备液，再依次添加助溶剂：(为保证实验结果的可靠性，澄的储备液可以根据储存条件，适当保存；体内实验的⼯作液，建议您现⽤现配，当天使⽤；以下溶剂前显⽰的百分⽐指该溶剂在您配制终溶液中的体积占⽐；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的⽅式助溶)1/2MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemE1.请依序添加每种溶剂：10%DMSO>>40%PEG300>>5%Tween-80>>45%salineSolubility:≥2.5mg/mL(4.95mM);Clearsolution2.请依序添加每种溶剂：10%DMSO>>90%(20%SBE-β-CDinsaline)Solubility:≥2.5mg/mL(4.95mM);Clearsolution3.请依序添加每种溶剂：10%DMSO>>90%cornoilSolubility:≥2.5mg/mL(4.95mM);ClearsolutionBIOLOGICALACTIVITY⽣物活性Adavivint(SM04690;Lorecivivint)⼀种有效的，选择性的典型的Wnt信号通路抑制剂，在SW480细胞中，⽤⾼通量TCF/LEF报告因实验，测得EC50值为19.5nM。IC50&TargetEC50:19.5nM(Wnt,SW480cell)[1]体外研究Adavivint(SM04690)isapotentandselectiveinhibitorofWntsignaling,withanEC50of19.5nMviaahigh-throughputTCF/LEF-reporterassayinSW480coloncancercells,andshowsnoeffectonSV40luciferasereporter.Adavivintenhancesaggregationofhumanmesenchymalstemcells(hMSCs)withanEC50of10nM.Adavivint(30nM)protectschondrocytesfromcatabolicbreakdowninvitro[1].体内研究Adavivint(0.3μg)enhancescartilagerepairandprotectionintheratacutecruciateligamenttearandpartialmedialmeniscectomyosteoarthritis(ACLT+pMMxOA)model[1].户使⽤本产品发表的科研⽂献•CellOncol.2022Oct21.•MolCarcinog.2022Oct12.•Bone.2022Feb23;116372.Seemorecustomervalidationsonwww.MedChemEREFERENCES[1].DeshmukhV,etal.Asmall-moleculeinhibitoroftheWntpathway(SM04690)asapotentialdiseasemodifyingagentforthetreatmentofosteoarthritisoftheknee.OsteoarthritisCartilage.2018Jan;26(1):18-27.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresear