Tunicamycin-DataSheet-生命科学试剂-MedChemExpress

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemETunicamycinCat.No.:HY-A0098CASNo.:11089-65-9分⼦式:C₃₉H₆₄N₄O₁₆作⽤靶点:Bacterial;Fungal;InfluenzaVirus;Antibiotic作⽤通路:Anti-infection储存⽅式:Powder-20°C3yearsInsolvent-80°C6months-20°C1month溶解性数据体外实验DMSO:40mg/mL(Needultrasonic)H2O:<0.1mg/mL(insoluble)请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存⽅式和期限：-80°C,6months;-20°C,1month。-80°C储存时，请在6个⽉内使⽤，-20°C储存时，请在1个⽉内使⽤。体内实验请根据您的实验动物和给药⽅式选择适当的溶解⽅案。以下溶解⽅案都请先按照InVitro⽅式配制澄的储备液，再依次添加助溶剂：(为保证实验结果的可靠性，澄的储备液可以根据储存条件，适当保存；体内实验的⼯作液，建议您现⽤现配，当天使⽤；以下溶剂前显⽰的百分⽐指该溶剂在您配制终溶液中的体积占⽐；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的⽅式助溶)1.请依序添加每种溶剂：10%DMSO>>40%PEG300>>5%Tween-80>>45%salineSolubility:≥2mg/mL(InfinitymM);Clearsolution2.请依序添加每种溶剂：10%DMSO>>90%(20%SBE-β-CDinsaline)Solubility:≥2mg/mL(InfinitymM);Clearsolution3.请依序添加每种溶剂：10%DMSO>>90%cornoilSolubility:≥2mg/mL(InfinitymM);ClearsolutionBIOLOGICALACTIVITY⽣物活性Tunicamycin⼀种同核苷抗⽣素的混合物,可抑制N-糖化并阻断GlcNAc磷酸转移酶(GPT)。Tunicamycin引起细胞内质⽹(ER)中未折叠蛋⽩的积累并诱导ER应激，并导致DNA合成受阻和G1期细胞周1/2MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemE期停滞。Tunicamycin可抑制⾰兰⽒阳性细，酵母，真和病毒，并具有抗癌活性。Tunicamycin增加宫颈癌细胞中外泌体的释放。体外研究Tunicamycin(2µg/mL;24hours;CD44+/CD24-andoriginalMCF7cells)treatmentincreasesthesplicedXBP-1,ATF6nucleartranslocationlevelandCHOPproteinexpressioninCD44+/CD24-andoriginalMCF7cells[1].Tunicamycin-inducedERstresssuppressesCD44+/CD24-phenotypecellsubpopulationandinvitroinvasionandacceleratestumorosphoreformation.UndereffectofTunicamycin,theresultsshowthatinhibitedinvasion,increasedcelldeath,suppressedproliferationandreducedmigrationintheCD44+/CD24-andCD44+/CD24-richMCF7cellculture[1].WesternBlotAnalysisCellLine:CD44+/CD24-andoriginalMCF7cells[1]Concentration:2µg/mLIncubationTime:24hoursResult:IncreasedlevelofsplicedXBP-1,ATF6nucleartranslocationandCHOPproteinexpressionaredetectedinCD44+/CD24-andoriginalMCF7cells.体内研究Tunicamycin(0.1mg/kgor0.5mg/kg)treatmentdramaticallysuppressestumorgrowthintheCD133+/-MHCC97Lcellsxenograftmodel(BALB/c(nu/nu)mice)[2].户使⽤本产品发表的科研⽂献•Nature.2020Mar;579(7799):433-437.•NatCommun.2022Apr6;13(1):1853.•Hepatology.2022Oct11.•EMBOMolMed.2022Jan11;14(1):e14502.•Autophagy.2021Jun;17(6):1426-1447.Seemorecustomervalidationsonwww.MedChemEREFERENCES[1].HsuJL,etal.Tunicamycininducesresistancetocamptothecinandetoposideinhumanhepatocellularcarcinomacells:roleofcell-cyclearrestandGRP78.NaunynSchmiedebergsArchPharmacol.2009Nov;380(5):373-82.[2].HanC,etal.Endoplasmicreticulumstressinhibitscellcycleprogressionviainductionofp27inmelanomacells.CellSignal.2013Jan;25(1):144-9.[3].HouH,etal.DPAGT1/Akt/ABCG2pathwayinmouseXenograftmodelsofhumanhepatocellularcarcinoma.MolCancerTher.2013Dec;12(12):2874-84.[4].KathleenMMcAndrews,etal.Mechanismsassociatedwithbiogenesisofexosomesincancer.MolCancer.2019Mar30;18(1):52.McePdfHeight2/2MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemECaution:Producthasnotbeenfullyva