常用小型实验动物灌胃给药技术方法（Techniques for intragastric administration of small experimental animals）

常用小型实验动物灌胃给药技术方法（Techniquesforintragastricadministrationofsmallexperimentalanimals）1页维普资讯泸州医学院学报2oo4年第27卷第3期ofhizi.dumedicalcollegevd.27NO3硎[J].4结果5操作体会经上述方法所制成的标本如附图所示。该标本显示结构较多，内容复杂，要求操作者对该部分的理论非常熟悉在去除小脑中脚时，最好。边切边拉切口的深度与剥离的进程同步，将显示内。容全部暴露后，最后再用解剖刀修洁整齐；并注意保留三叉神经根和前庭蜗神经根，以便观察毗邻关系。修洁小脑下脚时，应该用解剖镊撕去部分纤维，使其与周边关系呈自然移行状。参考文献1。李忠华。王兴海主编。解剖学技术【圳第暖北京2。人民卫生出版挂。1997；57～印2。张朝佑主编。人体解剖学M【】。第版北京人民卫生2。出版社。1998；1215-1200附图：经剥制后的标本（~0os-l】一19收稿）常用小型实验动物灌胃给药技术方法介绍赵明德（啻蓦州『医学院实验动物科，四川泸州64~000）关键词小型动物我灌胃；培药方法中匿分类号Q33文献标识码B文章编号1000-2669（0o4）3-0258-02随着生命科学技术的发展，动物实验已成为医小鼠、大鼠灌胃器的来源有两个，一是从市面上学实验研究的基本方法。根据实验研究目的、实验购买特制的I2一L6号灌胃针。二是自制。自制方法动物的种类及药物剂型、荆量的不同，要求对实验动为：取L2号普通注射针头，首先将针头尖部去掉，然物实施不同的给药方法。尤其对于药物的毒理、药后用细锉刀把去掉部分锉平、锉光，再用沙纸磨光效的研究和药物剂量的确定，经口灌胃给药方法是滑，有条件时可在针尖处点焊成球型加工成有20。一种重要的途径。在国内文献上却很少见到具体的度弧度的灌胃针，连接第一或5ml的注射器。即成为描述。在此，作者将在实践中摸索出的一套实用于大小鼠的灌胃器。在打磨针头或加工弧度时用力一小鼠、大鼠、豚鼠、家兔经口灌胃给药的方法介绍如定要均匀，否则就会将针管弄扁，影响针管畅通。豚下鼠灌胃器，可采用大鼠灌胃器。1.2家兔灌胃器的制作1灌胃器具制作取号腰椎穿刺针加工方法同小鼠、大鼠灌I2。1.1小鼠、太鼠、豚鼠灌胃器的制作胃器的制作，针体长约9cm。Andconnect10ndorI1InotesEjector,thatis,rabbitstomachpump.Abouttheauthor:ZhaoMingde(1968,1),male,experimenterPageand2VIPinformationThirdstageZhaoMingde:commonlyusedsmallexperimentalanimalsintragastricadministrationtechniquesintroduced259TheMinistryopensthemouth.Then,therighthandtoholdagoodinfusionofliquidmedicinestomach2gastriclavageRightfromthemouthslowlywitharcinsertion.Iftheinsertionpositioniscorrect,2.1micewereorallyadministeredbygavage,andtheycouldseetherabbitsswallowingautomatically.Iftheinsertionwasnotright,theanimalswouldmoveFirstBaodinganimal,thatis,withtherighthandtoliftthetailofmice,whenplacedinratsshouldimmediatelypulloutthestomach,reinsertthestomachintothestomach,theneedleintothestomachCageorroughsurface,whenthemousestruggledforward,withthelefthandthumbdepthof15~18cm.Afterthemedicineisfinished,thestomachispulledoutslowly.Fingerandforefingerintwoearsafterneckskin,usethismethodtoflipthemouseintotherabbitIg,basicallynotthedruginjectionerrorPalm,straightenthehindlegs,withasmallfingertokeepthetailofmice.TracheainBaoding.Themaximumamountofintragastricadministrationofrabbitis80-15Oral/only.Intheprocessofmice,donotforcetoomuch,donotholdtheneck,soasnottosuffocate3mattersneedingattentionDeath.Thestomachisgentlypressedtothehead,sothatthemouthandesophagusbecomeoneAstraightline,andthenbygavageneedlegentlyalongtheroofwallintotheesophagus,whenadministered3.1miceandratsWhentheneedlereachesabout3cI'/L,itreachesthestomach.IfthepositionoftheneedleisplacedinBaoding,theanimalsmustbefixedsothattheanimalheadsdonotInsertthecorrectmouse,toswallowdrugs,gavageneedleinsertionpositionisnottoswingfreely,butcannotsqueezetheneck.ThesyringeneedlefacesinradiansIndeed,themicewillstrugglestronglyandmustpullouttheplug.Otherwise,theymaybeinsertedintotherightcorneroftheanimalbeforethedrugistaken,andthemovementsshouldbegentle.IlltreatmentThesubstancewasinjectedintothetrachea,causingdeathinmice.Aftertakingtheliquidmedicine,gentlypullouttheanimals,especiallytherats,orbitethemeasily.InsertintothestomachGastriclavageneedle.Themaximumintragastricadministrationtimewas0.4ml/10g.w.Needle,ifyouencounterresistance,youcannotcontinuetoentertheneedle,shouldbestopped2.2ratswereorallyadministeredbygavage,andtheneedleswerewaitingforautomaticswallowing.Otherwise,it'seasytodamageThemethodofcaptureinratsisbasicallythesameasthatinmice.Dependingonthelengthoftheanimal'sesophagus.Practicalexperience,wefound,suchascoveredwithapieceofwetgauzeintherat,3.2guineapigsTheutilitymodelhastheadvantagesofskidresistanceandsafetyforbeginners.Inordertoguineapigtemperamentisgentle,buttimid,easytobesurprisedTopreventratbite,wearcanvasgloves.Theratswerelargerandfrightened.So,whencatchingguineapigs,bequickandsoft.BaodingMobileYoucan'tpressyourtaillikeamouseinBaoding.Similarly,inthepreservationoftheobject,itshouldbecoveredwithagauzehead.Fixinganimals,tomakethemRatscannotbeforcedtoolargeandholdtheirneck,especiallyinthegrip,cannotbearbitraryswing.Whenthestomachisfilled,thestomachcannotbeinsertedTakespecialcareoftheBaodingrats.Thekeytothegastriclavageinratsisthelargeleftanddonotdamagetheesophagus.Theheadoftheratisfixedsothattheheaddoesnotswingatwill.Other3.3rabbitsThemethodofoperationisbasicallythesameasthatofmice.Fillthestomachneedleintothedepthof5cmBaodingrabbits,mustmakeitsheadcannotarbitrarilyswing,todoAbout。Themaximalcapacityofratswas2ml/lOOg.watonetime.Tothispoint,thelefthandtoforcestuckaroundthemouthoftherabbit.InsertintothestomachTwo3guineapigsareorallyadministeredbyintragastricadministration.Thepositionofthedeviceshouldbecorrect.Donotmissthetrachea.Puttheguineapigontheexperimentaltableandcoveritwithadrygauze,FixtheguineapigunderyourlefthandandpressitwithyourthumbandforefingerLetthemouthopen.Then,therighthandisholdingthepumpingirrigationstomachmedicine,alongthedolphinreferenceTheratpalateinsertedintotheesophaguswall.Ifthestomachisinsertedintothepositionissmooth,1.initiative.Modernmedicallaboratoryanimalscience[M].Beijing:People'smilitaryIndeed,theguineapigautomaticallyswallowsthesecondcause.Otherwise,theguineapigwouldmove,andthenpressthepress,September2000.307-314.Pulloutstomach,backintothestomach,gastriclavagedevicetothedepthof2.ZhuQinghua,ZhuQingfan.LaboratoryAnimalScience[M.Guangzhou:GuangdongUniversityEduc