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RabbitMonoclonalAntibodies--BetterToolsforBetterScience2022/11/221RabbitMonoclonalAntibodies20

AdvantagesofRabMAbTechnologyOverMouseMAbTechnologyHigherquality

HighaffinityandspecificityBroadapplicationsHighsuccessrateinmeetingcustomers’criteriaNovelEpitopesRecognitionofmouseorthologswithhighhomologyto humanantigensHigherthroughputStablehybridomasMultiplexHighhumanizationprobabilityHigherhomologytohumangermlinesequencethanmouseAinvitropositiveproof-of-conceptstudy2022/11/22AdvantagesofRabMAbTechnoloRabbitsRecognizeMoreEpitopesMouseRabbitMolecular-weightmarkerFibronectinProteasedigestedfibronectin123 1235miceand3rabbitsWesternAnalysisPolyseraImmunizedwithhumanfibronectin2022/11/22RabbitsRecognizeMoreEpitopeSupernatantFromRabbitHybridomaIsHighlyActiveInWesternblotABCD(A=1:5;B=1:50;C=1:500;D=1:1000)K8Anti-HumanKeratin8MAb2022/11/22SupernatantFromRabbitHybridARandomCollectionOfRabMAbsHaveExtremelyHighAffinitiesAntigen MAb Kon(1/M.sec) Koff(1/sec) Kd(M)ER EPIer39 2.58x105 2.45x10-3 9.5x10-9ER EPIer14 2.03x105 9.28x10-5 4.57x10-10ER EPIer26 1.72x105 3.37x10-5 2.19x10-10ER EPIer38 2.87x105 7.79x10-4 2.71x10-9PR EPIpr42 1.18x106 1.63x10-2 1.38x10-8PR EPIpr33 3.22x106 4.13x10-6 1.28x10-12PR EPIpr88 4.46x105 2.51x10-4 5.63x10-10C29 EPIc2902 7.61x104 2.88x10-3 3.78x10-8C29 EPIc2903 3.26x104 3.12x10-6 9.57x10-11C29 EPIc2905 1.36x105 4.96x10-4 3.65x10-9C29 EPIc2906 3.43x104 4.57x10-5 1.33x10-9

Herceptin Kd(M)= 5.0x10-9 $300MRituxan 8.0x10-9 $1.1BSynagis 1.0x10-9 $600MRemicade 2.0x10-10 $270M2022/11/22ARandomCollectionOfRabMAbsRabMAbForBetterDiagnosticsMouse1A6RabMAbHeattreateduntreated2022/11/22RabMAbForBetterDiagnosticsMRabbitMAbMouseMAbPhageMAbNonPrimaryAbRabMAb:MoreAccurateResults---ComparisonofrabbitMAb,mouseMAbandphageMAb50%falsepositive15%falsepositive5%falsepositive2022/11/22RabbitMAbMouseMAbPhageMAbNoFibrinDegradationProductsFibrin(Bloodclot)PlasminPlasminogenEndotheliaCellPAI-1tPA

PropertyApplicationtPARabMAb

clone8BindDiagnosticsclone15InhibitTherapeuticsclone22StimulateTherapeuticsclone29StrongstimulateTherapeuticsclone34BindDiagnostics

PAI-1RabMAb

clone2InhibitPAI-1/tPATherapeuticsclone7BindDiagnosticsclone10InhibitPAI-1/tPATherapeuticsclone12InhibitPAI-1/tPATherapeuticsclone14BindDiagnosticsOneProcess:MultipleRabMAbProductsPAI-1tPA2022/11/22FibrinFibrin(Bloodclot)PlasPathoMAbtoIdentifyTherapeuticMAbandDiseaseTargetTumorcellEmbryoniccellInflammatorytissueDegenerativetissueMembranefractionNuclearfraction…generatesupto10,000MAbsfromdiseasetissuesdirectly

YYYYYAntigensBioactivityTherapeuticMAbcandidatesDiseasetargetsCell-basedassays2022/11/22PathoMAbtoIdentifyTherapeutMAbsAgainstMouseMammaryTumorsRaisedFromASingleRabbit~7000hybridomaclonesgenerated: 157of1152antibodiesareusefulforimmunohistochemistry80antibodiespreferentiallyrecognizetumor 57antibodiesreactwithepitheliumcells 2antibodiesbindspecificfornecroticareasoftumor11antibodiesreactwithstroma 2antibodies staincell-celljunctions 5antibodies detectsecretedmolecules 10antibodiesreactwithmyoepithelialcells 2022/11/22MAbsAgainstMouseMammaryTumBlockingMAbsforTumorCellAdhesion2022/11/22BlockingMAbsforTumorCellAPrimarytumor(<20%expression)Lungmetastasis(100%expression)RabMAbToANovelIonChannelTumorAntigen

---

identifieddirectlyfromimmunizationofcancertissues2022/11/22Primarytumor(<20%expressionUsingSubtractivePathoMAbtoIdentifyMAbsSpecifictoDiseaseTissuesImmunizeRabbitwithLungCancerCellsScreenHybridomaAgainstLungCancerCellsBack-ScreenPositivesAgainstNormalLungCells100MAbs5MAbs2022/11/22UsingSubtractivePathoMAbtoAKidneySpecificAntibodyGeneratedbyPathoMAbBrainLungLiverKidney2022/11/22AKidneySpecificAntibodyGenProofOfPrincipleForHumanizedRabMAb---from75%to95%humansequence

HzdMAbRabMAb293b-6transfectants293untransfected2022/11/22ProofOfPrincipleForHumanizMarketDemandForRabMAbsSmallMoleculesPeptidesMTMProteinsMouseMAbsUnavailable-NovelResearchreagentsorleadsforresearch,drugdiscoveryanddiagnostics.MouseMAbsAvailable-Opportunityforbetterqualityfollow-onantibodiesHigherAffinityDiagnosticsNovelEpitopesHigherAffinitiesTherapeuticsAntibodyPairsResearchReagentAntibodyArrays2022/11/22MarketDemandForRabMAbsPeptiImproveTheProbabilityOfSuccessForDrugDiscoveryRabMAbTechnologyTargetID&ValidationProteinlevel,Clinical-directedtargetvalidationMultiplex,CompaniondiagnosticsNovelEpitope,HighaffinityTherapeuticleadsDiagnosticleads2022/11/22ImproveTheProbabilityOfSucSuccessRateOfRabMAbGeneration2022/11/22SuccessRateOfRabMAbGenerat

Customers2022/11/22

SummaryRabMAbisasuperiortechnologyplatformthatiswidelyacceptedbytheindustryEpitomicsenjoysrapidgrowthincustomersforresearch,diagnosticandtherapeuticfieldsRabbitMAbshavehighaffinity;Rabbithybridomasarestable;Thesuccessratetoobtain“desirable”MAbsishigh;Thethroughputcanbehigh.

2022/11/22SummaryRabMAbisasuperiorteRabbitMonoclonalAntibodies--BetterToolsforBetterScience2022/11/2221RabbitMonoclonalAntibodies20

AdvantagesofRabMAbTechnologyOverMouseMAbTechnologyHigherquality

HighaffinityandspecificityBroadapplicationsHighsuccessrateinmeetingcustomers’criteriaNovelEpitopesRecognitionofmouseorthologswithhighhomologyto humanantigensHigherthroughputStablehybridomasMultiplexHighhumanizationprobabilityHigherhomologytohumangermlinesequencethanmouseAinvitropositiveproof-of-conceptstudy2022/11/22AdvantagesofRabMAbTechnoloRabbitsRecognizeMoreEpitopesMouseRabbitMolecular-weightmarkerFibronectinProteasedigestedfibronectin123 1235miceand3rabbitsWesternAnalysisPolyseraImmunizedwithhumanfibronectin2022/11/22RabbitsRecognizeMoreEpitopeSupernatantFromRabbitHybridomaIsHighlyActiveInWesternblotABCD(A=1:5;B=1:50;C=1:500;D=1:1000)K8Anti-HumanKeratin8MAb2022/11/22SupernatantFromRabbitHybridARandomCollectionOfRabMAbsHaveExtremelyHighAffinitiesAntigen MAb Kon(1/M.sec) Koff(1/sec) Kd(M)ER EPIer39 2.58x105 2.45x10-3 9.5x10-9ER EPIer14 2.03x105 9.28x10-5 4.57x10-10ER EPIer26 1.72x105 3.37x10-5 2.19x10-10ER EPIer38 2.87x105 7.79x10-4 2.71x10-9PR EPIpr42 1.18x106 1.63x10-2 1.38x10-8PR EPIpr33 3.22x106 4.13x10-6 1.28x10-12PR EPIpr88 4.46x105 2.51x10-4 5.63x10-10C29 EPIc2902 7.61x104 2.88x10-3 3.78x10-8C29 EPIc2903 3.26x104 3.12x10-6 9.57x10-11C29 EPIc2905 1.36x105 4.96x10-4 3.65x10-9C29 EPIc2906 3.43x104 4.57x10-5 1.33x10-9

Herceptin Kd(M)= 5.0x10-9 $300MRituxan 8.0x10-9 $1.1BSynagis 1.0x10-9 $600MRemicade 2.0x10-10 $270M2022/11/22ARandomCollectionOfRabMAbsRabMAbForBetterDiagnosticsMouse1A6RabMAbHeattreateduntreated2022/11/22RabMAbForBetterDiagnosticsMRabbitMAbMouseMAbPhageMAbNonPrimaryAbRabMAb:MoreAccurateResults---ComparisonofrabbitMAb,mouseMAbandphageMAb50%falsepositive15%falsepositive5%falsepositive2022/11/22RabbitMAbMouseMAbPhageMAbNoFibrinDegradationProductsFibrin(Bloodclot)PlasminPlasminogenEndotheliaCellPAI-1tPA

PropertyApplicationtPARabMAb

clone8BindDiagnosticsclone15InhibitTherapeuticsclone22StimulateTherapeuticsclone29StrongstimulateTherapeuticsclone34BindDiagnostics

PAI-1RabMAb

clone2InhibitPAI-1/tPATherapeuticsclone7BindDiagnosticsclone10InhibitPAI-1/tPATherapeuticsclone12InhibitPAI-1/tPATherapeuticsclone14BindDiagnosticsOneProcess:MultipleRabMAbProductsPAI-1tPA2022/11/22FibrinFibrin(Bloodclot)PlasPathoMAbtoIdentifyTherapeuticMAbandDiseaseTargetTumorcellEmbryoniccellInflammatorytissueDegenerativetissueMembranefractionNuclearfraction…generatesupto10,000MAbsfromdiseasetissuesdirectly

YYYYYAntigensBioactivityTherapeuticMAbcandidatesDiseasetargetsCell-basedassays2022/11/22PathoMAbtoIdentifyTherapeutMAbsAgainstMouseMammaryTumorsRaisedFromASingleRabbit~7000hybridomaclonesgenerated: 157of1152antibodiesareusefulforimmunohistochemistry80antibodiespreferentiallyrecognizetumor 57antibodiesreactwithepitheliumcells 2antibodiesbindspecificfornecroticareasoftumor11antibodiesreactwithstroma 2antibodies staincell-celljunctions 5antibodies detectsecretedmolecules 10antibodiesreactwithmyoepithelialcells 2022/11/22MAbsAgainstMouseMammaryTumBlockingMAbsforTumorCellAdhesion2022/11/22BlockingMAbsforTumorCellAPrimarytumor(<20%expression)Lungmetastasis(100%expression)RabMAbToANovelIonChannelTumorAntigen

---

identifieddirectlyfromimmunizationofcancertissues2022/11/22Primarytumor(<20%expressionUsingSubtractivePathoMAbtoIdentifyMAbsSpecifictoDiseaseTissuesImmunizeRabbitwithLungCancerCellsScreenHybridomaAgainstLungCancerCellsBack-ScreenPositivesAgainstNormalLungCells100MAbs5MAbs2022/11/22UsingSubtractivePathoMAbtoAKidneySpecificAntibodyGeneratedbyPathoMAbBrainLungLiverKidney2022/11/22AKidneySpecificAntibodyGenProofOfPrincipleForHumanizedRabMAb---from75%to95%humansequence

HzdMAbRabMAb293b-6transfectants293untransfected2022/11/22ProofOfPrincipleForHumanizMarketDemandForRabMAbsSmallMoleculesPeptidesMTMProteinsMouseMAbsUnavailable-NovelResear

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