作用机理及实际使用结果

LEVUCELL®

SB产品简介办事处拉曼公司秦鹏一、二、的作用机理

在猪上的应用Levucell

SB

®Saccharomyces

cerevisiae

boulardiiUnique

Eucaryote

Probiotics®酵母

独特的真核益生菌FAO/WHO mended

definition

:‘Live

microorganisms

which

when

administered

in

adequateamounts

confer

a

health

benefit

on

the

host’粮农组织/世界卫生组织

定义活的微生物，当摄入足够数量时有益于宿主健康Lactobacillus

acidophilus

R0052Saccharomyces

cerevisiae

boulardii

CNCM

I-10792014年 食品药品管理局FDA和 饲料

AAFCO公布添加动物饲料中的微生物添加剂48中。2013年农业部饲料添加剂品种 了34种微生物饲料添加剂地衣芽孢杆菌枯草芽孢杆菌粪肠球菌屎肠球菌两歧双歧杆菌乳酸肠球菌嗜酸乳杆菌干酪乳杆菌乳酸乳杆菌10.植物乳杆菌乳酸片球菌戊糖片球菌产朊假丝酵母酿酒酵母沼泽红假单胞菌…….等等Lactobacilli

乳酸菌Lactobacillus

acidophilus嗜酸乳杆菌Lactobacillus

casei干酪乳杆菌

Lactobacillus

delbrueckii

subsp.bulgaricus德氏乳杆菌保加利亚亚种

Lactobacillus

delbrueckii

subsp.lactis德氏乳杆菌乳酸亚种Lactobacillus

plantarum植物乳杆菌Lactobacillus

helveticus

乳酸杆菌

Lactobacillus

fermentum发酵乳杆菌

Lactobacillus

reuteri罗氏乳酸杆菌Yeast

酵母菌Saccharomyces

boulardii

酵母Bifidobacteria

双歧杆菌Bifidobacterium

adolescentis青春双岐杆菌Lactobacillus

rhamnosus鼠李糖乳杆菌Bifidobacterium

bifidum两岐双岐杆菌Bifidobacterium

breve短双岐杆菌

Bifidobacterium

infantis婴儿双岐杆菌

Bifidobacterium

longum长双歧杆菌Other

Genus

其他种类Streptococcus

salivarius

subsp

thermophilus链球菌嗜热亚种Bacillus

subtilis枯草芽孢杆菌

Bacillus

coagulans凝结芽孢杆菌

Pediococcus

acidilactici乳酸片球菌Enterococcus

faecium屎肠球菌EFSA

scientific

has

so

far

rejected

260

claims

on

probiotics

in

Europe

due

to

insufficient

researchand

thus

inconclusive

proof

_cited

欧洲食品/饲料安全局驳回260多个益生菌申请酿酒酵母并不相同！10

microns

=

invisible

to

the

naked

eye10

微米 肉眼无法辨别Different

genus

菌种不同Saccharomyces

酿酒酵母Kluyveromyces

鹰嘴豆孢克鲁维酵Different

genomes

different

speicies不同的DN

段产生不同的菌株Different

metabolite

代谢活性和产物Different

applications

应用方向Bread

面包Beer

啤酒Wine

葡萄酒Feed

supplement

饲料添加剂Saccharomyces

cerevisiae

yeasts

representthousands

of

strains

with

different

genomes.酿酒酵母种包含上千不同 的菌株Specific

strain

Saccharomyces

cerevisiae

boulardii

CNCMI-1079

in

Pasteur

InstituteS.c.boulardii

is

a

subspecie

of

Saccharomyces

cerevisiae

with

unique

metabolicandgenetic

properties

whi ake

it

as

the

unique

Eucaryote

probiotics®高浓度活性

酵母，巴斯德菌种保存中心

CNCM

I‐1079；属于酿酒酵母独特的亚种，具有特有的遗传片段、代谢产物和应用价值；酵母是益生菌范畴中唯一的酵母菌。Efsa

：COMMISSION

REGULATION

(EC)

No

2036/2005COMMISSION

REGULATION

(EC)

No

1847/2003DNA

Fingerprint

identify

S.c.boulardii

as

different

toother

strains

of

S.cerevisiae.DNA图谱显示：菌属于酿酒酵母亚种菌不同于普通酿酒酵母SCI

3.4

(2011)®人和单胃动物健康和营养的活酵母菌株1962199320031920monogastric

nutritionand

healthSelection

ofSaccharomyces

c.boulardii.

Used

as

ahuman

preventivedrug

for

diarrhea菌株预防和治疗人类腹泻饲料添加剂

改善单胃动物营养健康Development

ofSaccharomyces

c.boulardii

as

feedadditive

for超过250篇单胃动物献应用的国际期More

than

250InternationalReferences

onmonogastrics东南亚以热带水果茶治疗腹泻Native

Indochinanscure

theirdiarrhea

bydrinking

tea

madewith

tropical

fruits治疗ysis

of

Saccharomyces.

Lynne

VSystematic

review

and

meta‐McFarland.2010Infectious

Diseases

Evidence‐based

Guidelines

for

the

Management

of

AcuteGastroenteritis

in

Children

in

Europe

Update

2014影响因子2.4711st

LINE

OF

DEFENSE

:REGULATION

OF

INTESTINAL

MICROFLORA第1道防线:调整肠道微生物菌群Oxygen

consumption清除残余氧Stimulation

of

positivemicroflora刺激有益菌增殖Adhesion粘附作用Reduced

atta ent

ofundesirable

bacteria减少病原菌附着Neutralisation中和作用Unique

mode

of

actionNeutralisation

ofmicrobial

toxins(C.difficile,

E.coli,

...)特异性细菌毒素中和功能(24)

Ghent

University,

2001.

Effect

of

Levucell

Sb

on

histomorphology

of

epithelial

cells.刺激乳酸菌增殖并抑制有害菌的发育(24).7,576,565,554,54菌数Log

10

cfu/g+

1.2

log-

0.7

log-

0.7

log-

0.6

log空白对照组Faster

O2

consumption

with

LEVUCELL®SB

than

with

the

other

yeast

strains比其他酵母菌株的耗氧速度更快LEVUCELL®

SB

role

in

the

hindgut

在后肠的作用➥Oxygen

scavenging

in

the

caecum

and

thecolon.

盲肠和结肠中快速耗氧➥Better

anaerobic

conditions.较好的厌氧环境➥Better

fermentation

rate.较高的发酵速度➥

More

energy

released

from

the

same

diet

shorter

time

(SCFA

>energy).相同日粮通过锻炼脂肪酸异生供能提供 可利用能量The

Levu

a

quick

testfor

evaluating

O2

consumptionby

active

yeast

in

a

solutioncontaining

a

specific

dye.When

all

O2

is

consumed,

thesolution e

colorless.YeastMannoprotein

layyeerr-glucan

laayyeer

rPhospholipic

membraneCytoplasmic

compoundsThe

outer

membrane

of

S.boulardii

is

richer

inmannose

(bind

pathogenic

bacteria

thatcontains

Type

I

fimbria)than

other

S.cerevisiaeGedek

B.R,

Intestinal

flora

and

bioregulation.

Bull

Sci

tecoff

Inter

Epizoot1989与普通酿酒酵母相比，酵母细胞壁富含

甘露聚糖细菌类益生菌和普通酿酒酵母均不能有效吸附结合有害菌粘膜蛋白酶不会产生毒素的梭菌(C.difficile)维生素及其他物质水解水解毒素α毒素β会产生毒素的梭菌(C.difficile)_毒素α毒素β+__1

(8,9)+2

(10)3

(11)IgA4

(12,13)5(8,9)

Castagliuolo

I.

:

1996,

1999

;

Infect

Immun.Czerucka

D.,

1999

:

GastroenterolCorthier

G.,

1988,

1999

:

Infect

Immun.S

c.

boulardii

:产生蛋白酶破坏梭菌产生的毒素强化肠道上皮细胞将产毒梭菌转变为不产毒梭菌间接提高免疫球蛋白IgA

的产生维生素及其他物质抑制梭毒素的SaccharomycescerevisiaeboulardiiQamar,

AL:S2B0.0T1D;.IEnfNecGt

.Im02m2u0n.04Rodrigues

A.C.P.,

2000

:

Journal

of

Applied

MicrobiologyS.c.boulardii

remove

bacteria

toxin

by

binding

effects通过粘附作用有效去除细菌毒素的影响Page

32ENDOTOXIN

内毒素Integral

partof

cell

wall

细菌壁的主要组成部分Protein

and

lipid

structure

LPS

磷脂多糖蛋白質復合物Many

effects

on

host

多效影响宿主Produced

byGram‐negative

organisms

only

只由革兰氏菌生成Administration

of

probiotics

influencesF4

(K88)‐positive

enterotoxigenic

Escherichiacoli

atta

ent

&

intestinal

cytokine

expression

in

weaned

pigsDate

of

publication:

2011Journal:

Veterinary

Research,

42:

69口服益生菌对产肠毒素F4(K88)大肠杆菌在断奶仔猪肠道粘附作用和白介素表达的影响不用抗生素或益生菌的对照组（CTRL）；添加金霉素和泰妙菌素的抗生素组；乳酸菌组；组；5.

+乳酸片球菌组添加 显著降低附着在仔猪肠粘膜上的产肠毒素大肠杆菌ETEC

F4

(P=0.03)Date

of

publication:

2010Journal:

Journal

of

Animal

Science,

89:

52‐58组（200g/T）各15头，饲喂16天26日龄断奶仔猪，对照组和采食LPS攻毒：静脉注射LPS（25μg/kg体重）组仔猪血液白细胞、淋巴细胞和中性粒细胞水平显著高于对照组（P＜0.05）采食量： 处理组仔猪采食量增加39.9%（P＜0.05）；率：LPS攻毒后 率比对照组下降了20%；•白细胞介素：肝坏死因子：仔猪的IL‐1β和IL‐6的峰值较低（P＜0.05）；仔猪的TNF‐α和TNF‐γ的峰值提前，较高的峰值持续时间更长。口服降低埃希氏大肠杆菌内毒素诱发免疫和皮质醇反应导致的仔猪Oral

administration

of

Saccharomyces

cerevisiae

boulardii

reduces

mortalityassociated

with

immune

and

cortisol

responses

to

Escherichia

coli

endotoxin

in

pigs.Authors:

Collier

C.T.,

Carroll

J.A.,

Ballou

M.A.,

Starkey

J.D.,

Sparks

J.C.Page

31Intestinal

morphology肠道形态学2nd

LINE

OF

DEFENSE

:MAINTENANCE

OF

GOOD

INTESTINALSTRUCTURE

AND

FUNCTION第二道防线：维持肠道正常结构和功能Improved

villus

heightand

crypt

depth增加绒毛长度、隐窝深度Tight

junctionsRegulation调节紧密连接Improved

barrier

function.维持肠道上皮天然屏障功能(University

of

Nottingham

2012)(University

of

Milan

2002)Tight

junction

紧密连接跨膜蛋白跨膜蛋白紧密连接粘附因子胞质附着蛋白ZO肌球蛋白肌动蛋白强化肠道屏障功能25Maintain

epithelia

structure

(Bontempo

et

al.,2006)维持上皮结构Stabilise

tight

junctionsIn

vitro:Czerucka,

et

al.

(2000)In

vivo

(porcelets):Watts

et

al.

(unpublished)稳定紧密连接Reduction

of

pathogens

translocation(Lessard,

et

al.

2008,

Martinset

al.

2010)减少病原菌移位Increase

intestinal

IgA

(Lessard

et

al.

2008,Buts,

1990)增加肠道粘膜免疫抗体IgAControlSCB+

EPEC肠致病型大肠埃希菌SCB+

EPEC®+肠致病型大肠埃希菌3rdLINE

OF

DEFENSE

:ANTI-INFLAMMATORY

AND

IMMUNE

PROPERTIES第三道防线：抗炎性和免疫特性Less

piglets

mortality

followinga

LPS

endotoxin

challenge脂多糖内毒素攻毒后仔猪

率降低Less

inflammation:decreasedserum

concentration

of

IL-1β(pro-inflammatory

cytokine)

降低炎性反应减少

中促炎性白介素浓度Collier

et

al.

2010.27抗生素生长促进剂的非抗生素抗炎性效果，作用模式假说Healthy

piglets:

increased

TLRexpression

(antigen

recognition)

LeBon

et

al.

(2013)健康仔猪：提高T受体蛋白（TLR）的表达，促进抗原物质的识别。Homeostasis

re‐inforcement强化稳态平衡Under

challenge:

control

ofinflammation

and

host

response(Martins

et

al.

2010;

Collier

et

al.

2011)应激状态：控制炎症程度和宿主反应Salmonella

n=10

Mice沙门氏菌攻毒LPS

injectionn

=

15

piglets大肠杆菌内毒素攻毒Reduced

mortality减少

率28白细胞计数LPS攻毒后时间2

9白介素1β白介素6嗜中性粒细胞氧化爆发γ-干扰素酵母与人类健康Antibiotic‐associateddiarrhea

抗生素相关性腹泻Recurrent

Clostridium

difficile

infection

艰难梭菌诱发症Irritable

bowel

syndrome

肠激惹综合征Inflammatory

bowel

disease

慢性非特异性肠道炎Travelers‘diarrhea

差旅型腹泻Acute

diarrhea

急性腹泻HIV/AIDS‐associated

diarrhea

相关腹泻AAD的定义应用抗生素后继发的腹泻。抗生素破坏肠道内菌群平衡，金黄色葡萄球菌、沙门氏菌、产气荚膜杆菌、克雷伯杆菌、白色念珠菌、难辨梭状芽孢杆菌等增殖，

因抗生素诱因不同而异

5~40％。AAD的诱因万古霉素外的抗生素均可诱发AAD，广谱比窄谱抗生素诱发率高10-70倍是临床预防和治疗主要方案=使用抗生素时需要辅助占伪膜性结肠炎发病诱因95%，该病率高达24艰难梭菌(Clostridiumdifficile)％。是临床试验唯一具有显著疗效的方案706050403020100Fréquency

(%)Prevalence

of

C.

difficile

inneonatal

diarrhea

diagnosticfor

piglets艰难梭菌在新生仔猪腹泻时非常常见Yaeger

M.,

2001,

Iowa

UniversitySonger

JG.

:2000

;

Swine

Health

Prod

8:185‐189.DeLay

J.

:

2002

;

AHL

Newsletter

–

Vol

4,

Issue

3C.

difficile

is

identified

with

monogastric

species

:

human,

horses,

dogs,

cats…

(6)梭菌广泛存在于很多单胃动物体内：人类、猪、鸡、马、猫…(6）Worldwide

stats

point

alarming

increases

of

C.

difficile

in

case

of

neonatal

diarrhea

(4,

5)全球统计显示,梭菌造成新生仔猪下痢的问题有日益严重的趋势(4,

5)60%腹泻与艰难梭菌相关Only

pathogen单一病原菌Pathogen

associated复合型腹泻Lebret

A.

:

2002

RIPP

Oral

presentation.Castex

:

1990

;

Gen.

Microbiol.

136

;

1085‐1089Effect

of

C.

difficile

on

the

villi

of

the

jejunumC.

difficile

remains

even

with

classical

antibiotic

(amoxicilline,

colistine…)(7)传统抗生素（阿莫西林、硫酸粘杆素…）对艰难梭菌无效(7).Toxins

of

C.

difficile

destructs

the

Intestinal

Mucosa

(8).艰难梭菌毒素破坏粘膜(8)Castex,

19892006年， 。63个猪场，867个粪便样品，取自20周以上的生长育肥猪。数据来源：Clostridium

difficile

on

U.S.Swine

Operations.

农业部

.

2011年5月 数据。这个比例远高于正常成人艰难梭菌带菌率。2. Cell

wall

composition

细胞壁结构Natural to

pelleting

酵母更耐受制粒工艺Resistant

to

antibiotics

酵母菌天生耐受抗生素Ability

to

transfer

genetic

酵母无抗生素耐药性传递风险Natural

Occurrence

in

gut

天然肠道菌群(活性更重要)Ability

to

colonize

定殖力(无潜在风险）Synergistic

effects

on

other

microbes

菌群协同增效Application

as

probiotics

益生菌应用Ability

to

produce

antagonistic

compounds产生拮抗不良产物的风险Ability

to

neutralizeenterotoxin中和细菌内毒素的能力安全的益生菌乳酸杆菌乳酸球菌双歧杆菌酵母益生菌非安全屎肠球菌粪链球菌芽孢杆菌其他芽孢菌链球菌短期医嘱用药不适宜长期使用益生菌安全问题益生菌安全性EFSA

2012

scientificopinion

list

QPS

micro

organisms

2680:

A

WORLD

LEADER

IN

YEASTAND

BACTERIA

PRODUCTION世界顶尖的酵母和细菌生产供应商Global

leader

in

microbial

selection

and

screening:➥

More

than

1,000

yeast

strains

in

Lallemand

culture

collection.拉曼拥有上千株菌株的微生物种质资源State

of

the

artproduction

processes

for

microbial

products:➥More

than

25

specialized

yeast

&

bacteria

production

plantsaround

the

world.全球超过25家专业化的酵母和细菌发酵工厂Effective

animal

research

and

biotechnology

network:➥

3

labs

fully

dedicated

to

screening,

processing

and

stability,三所

致力于菌株筛选、加工和稳定性➥

Research

partnerships

with

world-class

expertise

centres:University

of

Nottingham,INRA,BRI…世界顶尖的研究机构长期合作研究诺丁汉大学、法国农业

、 国家生物技术

…..Identity

and

Selection

鉴定与筛选1920.

独特疗效促使菌株被发现Identification

of

the

active

ingredient

of

a

traditional

Asian

medicine

(infusion

of

lycheehulls)

against

infant

diarrhea

:

Dr

Boulard

discovered

and

isolated

a

specific

yeaststrain

and

named

it«boulardii».1962.制成治疗人腹泻用药，展开大量科研工作form

of

boulardii

active

yeast

recognized

and

marketed

as

a

probiotic

in

humanmedicine.

Since

then,

a

huge

amount

of

fundamental

research

on

mode

of

action

andapplication

of

boulardii

in

human

medicine

has

been

generated.1993.

欧盟

应用于动物EU

registration

of

a

S.c.boulardii

(CNCM

I-1079

/

LEVUCELL®

SB)

specific

activeyeast

for

use

as

a

gut

flora

stabilizer

in

animal

nutrition.1997.确定微生物分类学归属boulardii

wasclassified

as

a

variety

of

Saccharomyces

cerevisiae.

Its

genetic

similarityand

its

functional

differences

are

recognized.Today,已经获得44个国家批准使用LEVUCELL®

SB

is

authorized

and

marketed

in

more

than

44

countries.Antibiotics抗生素Prostable

SB(batch

00116)Dosecommonlyused

in

animalnutrition常规用量Compatibility配伍性Flavomycin

黄霉素Zinc

Bacitracin

杆菌肽Tylosin

tartrate

泰乐菌素Stafac(40%Virginiamycin)速大肥Carbadox

卡巴氧Sodiumsulfadimethoxine

磺胺地索辛钠Furazolidone

呋喃唑酮Chlortetracycline

HCI

金霉素Procaine

Penicillin

G

青霉素普鲁卡因Olaquindox

喹乙醇Flubendazole

氟苯咪唑Colistin

粘杆菌素Amoxicillin

阿莫西林Oxolinic

acid

奥索利酸Oxytetracycline

土霉素Tiamulin

泰妙菌素Tylosin

泰乐菌素ycin

林可霉素1600

ppm1200

ppm1000

ppm200

ppm1600

ppm1600

ppm1200

ppm1000

ppm1600

ppm400

ppm48

ppm560

ppm800

ppm800

ppm1440ppm200

ppm720

ppm220

ppm20

ppm20

ppm200

ppm40

ppm20

to

50

ppm400

ppm300

ppm500

ppm400

ppm100

ppm12

ppm120

ppm400

ppm400

ppm720

ppm100

ppm360

ppm110

ppmCompatibleCompatibleCompatibleCompatibleCompatibleCompatibleCompatibleCompatibleCompatibleCompatibleCompatibleCompatibleCompatibleCompatibleCompatibleCompatibleCompatibleCompatibleLEVUCELL

SB

is

fully

compatible

with

ATB

and

with

most

organic

acids可以和抗生素及大部分有机酸配伍Antibiotics

act

against

bacteria

buthave

no

effect

on

live

yeast

cells.抗生素是针对细菌而研发生产的，对活性酵母细胞无

。STABILITY

OF

LEVUCELL®WITH

CHEMICALCOMPOUNDS有机酸抗生素稳定1~3个月稳定1~3个月稳定<1个月稳定1~3个月Live

cellCoating

matrixZoom

on

TITAN

Technology聚焦铁达保护技术The

TITAN

technology

is

coming

from

2

research

axis

:铁达保护技术的成功是两个研究方向的共同成果2/

Development

of

a

specific

coating

technologyincreasing

the of

the

yeast

to

external

stresses

while

preservingan

optimal

bioavailability

forthe

animal.LEVUCELL®S在猪上的应用1/PIGLET

DIARRHOEA

CONTROL

控制仔猪腹泻2/

PERI-PARTUM

CHALLENGES

围产期的3/PRODUCT

INFORMATION

产品信息1LEVUCELL®

SB:

PIGLET

DIARRHOEA

CONTROL®

：控制乳仔猪腹泻Pre‐weaningPost‐weaningFatteningBrachyspira

hyodysenteriae

猪痢疾短螺旋体‐‐+Brachyspira

pilosicoli毛肠短状螺旋体‐±+Clostridium

difficile艰难梭菌+++‐‐Clostridium

perfringens

type

A

产气荚膜梭菌A+++±‐Clostridium

perfringens

type

C产气荚膜梭菌C+‐‐Enterococcus

durans

耐久肠球菌+?‐‐Escherichia

coli(AEEC)脱落性大肠杆菌+++±?‐Escherichia

coli

(ETEC)猪肠毒性大肠杆菌++++±Lawsonia

intracellularis劳索尼亚氏细胞内寄生菌‐++++Salmonella

typhimurium:鼠伤寒沙门氏‐++Cryptosporidium

spp.隐孢子虫属‐±‐Isospora

suis

(coccidiosis)猪同形球虫+++±‐GET

(corona)传染性胃肠炎冠状‐‐‐PED

(corona)流行性腹泻冠状±±±Rotaspp.环状+++‐Non‐specific

colitis

非特异性结肠炎*:

may

vary

according

to

country

and

ti‐me

After

Martineau

an

±

van

(2dMor0104)5+Bacteria细菌Protozoa原虫esLevucell

SB

against

Neonatal

Diarrhoea母猪添加

避免抗新生仔猪腹泻Farm

description:猪场背景600

sows

unit

near

Le

Mans24

sows

farrowing

each

weekmultiplier

herd

producing

replacement

gilts

for

anothers

(same

owner)Weaning/selling

piglets

at

4

weeks

of

age法国勒芒，600头能繁母猪，每周24头分娩种猪群为

其它猪场提供生产繁殖母猪（二元）仔猪4周龄断奶，随即销售History

of

neonatal

diarrhoea

and

agalactiadespite

vaccination

against

farm

specific

E.

Coli

&

huge

antibiotic

use猪场

新生仔猪腹泻和哺乳母猪无乳症的困扰尽管注射特异大肠杆菌

并且使用了大量抗生素，仍不见任何起色。Levucell

SB

against

Neonatal

Diarrhoea母猪添加

避免抗新生仔猪腹泻Feces

frompiglets

showing

diarrhea

checked

for

contamination

with

C.

difficile

(toxin)&Rotaby

Ms.

Helen

Davies,

PhD,

Univ.

Nottingham仔猪粪便送至诺丁汉大学检测艰难梭菌毒素和轮状100%samples

positive

to

C.

difficile

toxins

粪便样本呈现梭菌毒素阳性48%samples

positive

to

rota as

well粪便样本呈现轮状June‐July

2011,

5

consecutive

groups,

individual

follow‐up

of

piglets

the 10

days

of

life2011年6月起，连续观察5批分娩母猪，新生仔猪10天内

记录2

treatments

applied

to

sows

母猪料槽补饲negative；+30

g/d

FarmPack

~4.5

g

Levucell

SB

20

for

2

weeksGestation

diet妊娠料Peri‐partum

diet围产期料Lactation

diet泌乳料sows

moved

tofarrowing

room母猪转产房SowSelection母猪挑选Farrow分娩weighpiglets仔猪称重weighpiglets仔猪称重Weaning断奶Levucell

SB

topdressf+5d产后5天14dindividual

piglet

fecal

score

；

am/pm

(10

days)仔猪

粪便取样、评分：每天2次，连续10天Reduction

of

neonatal

diarrhoea:%of

piglets

showing

liquidfaeces

新生仔猪腹泻减少：水样便出现率%40

litters

/

treatment每组观察40窝仔猪Rota轮状C.

Difficile

toxins艰难梭菌毒素E.Coli

etc大肠杆菌等Conclusions

结论Levucell

SB

didn’t

prevent

rota

associated

diarrhea

(1stday

of

life)®未预防轮状 诱发的腹泻（第1天）It

protected

piglets

against

toxins

of

Clostridium

difficile(days

2,

3,

4)®抵御难梭菌毒素对仔猪的危害（第2~4天）In

consequence

recurrence

of

diarrhea

due

to

co‐infectionwith

E.

Coli

was

reduced®

减少继发性大肠杆菌

导致的腹泻出生1周内的腹泻发生天数平均日增重ADG

(g)76543210579

1113151719CTRLCTRL+Tiamulin

topiglets+Tiamulin

to

piglets+boulardii

to

the

sow+boulardii

to

the

sowTRIAL

ITRIAL

II断奶后腹泻%

diarrhea

post‐weaning%

diarrhea

days0‐5新生仔猪腹泻It’s

important

to

reduce

digestive

troubles

from

the

very

start

!从仔猪出生开始降低消化道疾病风险至关重要5

2SUPPORTING

PIGLETSHEALTH

STATUS53WHAT

ABOUT

PERFORMANCES

?对猪只饲养表现如何？Field

experience

:

farrow

to

fininsh

farm

(2000

sows)

in

France法国2000头能繁母猪的猪场，仔猪从出生养至育肥上市Historical

problems

with

Oedema

Disease

长年受到仔猪水肿病的困扰Repeated

failures

with

classical

approaches

(Colistine,

organic

acids,

…)-多次尝试常规方案，如使用硫酸粘杆菌素，有机酸处理等，但均未奏效Start

with

S.boulardii

on

batch

3

(below

chart)

and

situation

clearly

improved使用

后，猪场明显改观率的变化对生乳仔猪饲料中使用长性能的影响55Trail

performed

in

Switzerland

(2011)实验在2011年的 进行Location:

Provimi

Kliba/Cargill

Switzerland嘉吉普乐维美Kliba公司Commercial

farm

used

to

run

well

controlled

trials

(piglets

individually

weighted….)可控性强的商业猪场，用于开展产品评估实验Animals:208

piglets

weaned

at

4

weeks

of

age

208头仔猪在4周龄断奶Feeding

program:

Mash

feed饲喂制度：粉料15

kg

of

Phase

1

feed(Starter)保育1期料15kgMilk

powder,Fish

Meal,extruded

grains…奶粉、鱼粉、挤压谷物patatoe

protein,

caroub

but

with

qui ow

Lysine

level

(1.20%

total

Lysine)豆蛋白、

caroub 但是赖氨酸水平只有1.2%followed

byPhase

2

feed

15kg保育2期料15kggrain

based

diets

(Barley,wheat,…)with

Soy

Bean

Meal

谷物豆粕型日粮Control

diet

and

Control

+

Levucell

SB

added

at

2

x

106

CFU/g

of

feed对照组

vs 组

(2E+6

cfu/g）56Group组别Control对照组Levucell

SBADG

(g/d)平均日增重208234**+12.5%FCR

(kg/kg)饲料转化效率1.791.57‐12.3%FI

(g/d)饲料采食量362368+4

gPhase

1

断奶后两周内weaning)( 14

days

after57Group组别Control对照组Levucell

SBADG

(g/d)平均日增重357416**+19.3%FCR

(kg/kg)饲料效率1.791.72‐4%FI

(g/d)饲料采食量639718+79

gPhase

2

(

21

days)断奶后第3周到第5周Phase

1

+

Phase

2(35

days)断奶至实验末Group组别Control对照组Levucell

SBADG

(g/d)平均日增重460544**+18%FCR

(kg/kg)饲料效率1.811.74‐4%FI

(g/d)饲料采食量832947+115

g**

p<0.05Significant

effect

of

Levucell

SB

on

the

ADG

and

on

Feed

Efficiency显著改善平均日增重和饲料效率乳猪商品料知名企业：21天断奶至35天,89

头实验仔猪对照组Control组LSB结果results采食量Feed

intake(g)230239+3.8%日增重ADG

(g)210231+10%

*知名猪料试验于2012年11月份120头37日龄‐57日龄仔猪添加剂量LSB

120g/吨指标parameters对照组Control组LSB差异Difference日均采食Feed

intake872905+33日增重ADG507562+55料肉比FCR1.721.61‐0.11腹泻频次diarrhea

frequency33%8%‐25欧洲某根据下面的选择依据已经对许多益生菌进行过筛选12again

antibiotics,

抗生素敏感性again

acidification,酸化剂敏感性ability

to

promote

cellulolitic

flora,纤维分解菌促进能力ability

to

promo actic

flora,乳酸菌的促进能力direct

property

to

struggle

again

E.Coli

直接抵抗大肠杆菌的能力direct

effect

on

inflamation

对炎症反应的直接效果heat during

processing

饲料加工工艺的热稳定性酵母最终胜出2LEVUCELL®

SB:

PERI‐PARTUM

CHALLENGES：围产期的：主要应用方式和作用效果WHAT

HAPPENS

ATOF

GESTATION?母猪在妊娠后期的变化Changes

in

housing

conditions.房舍改变Changes

infeeding

programs

饲料和饲喂制度改变(diet

composition

and

allowance).Deep

hormonal

changes.激素变化Reduced

intra-abdominal

room.消化道蠕动空间下降Farrowing:intensive

muscular

effort.分娩：肌肉努责分娩配种断奶应激Transit

slowdown.蠕动变缓Constipation.便秘Increased

intestinalmembrane

permeability肠粘膜通透性增加Unbalanced

microflora.菌群失衡Pathogenic

bacteria

development.病原菌繁殖Toxin

production

&

release毒素产生并Mechanical

andphysiologicalConsequences机械和生理性改变Hormonal

consequencesCortisol

production激素改变、皮质醇Microbial

andPhysiologicalConsequences微生物和生理性改变LEVUCELL®SB:

MainApplications

and

Roles：主要应用方式和作用效果NEGATIVE

CONSEQUENCES

OF

STRESS应激的不良Reduced

feed

intake,采食量下降Longer

farrowing

processes,

产程延长Impaired

colostrum

quality,初乳品质下降La actation

set

up(MMA/PFAS).开乳较晚Mortality

率:Diarrhoea,腹泻诱发Mortalityat

birth,出生死胎Crushed

piglets,挤压致死Weak

piglets,弱仔致死Weaned

piglets.

断奶后Growth

生长发育:Heterogeneity,窝差异大Growth

retardation,生长延滞Litter

weight

at

weaning.断奶窝重低：主要应用方式和作用效果WHAT

ARE

THE

HAZARDS

ASSOCIATED

TO

STRESS?应激会诱发什么危害？An

improved

response

tostress.Preparation,

Stabilisation,

and

Regulationof

DIGESTIVE

MICROFLORA

andTRANSIT

QUALITY

OF

SOWS改善动物对应激的反应准备、稳定和调节母猪的消化道微生物以及肠道食糜蠕动效果LEVUCELL®SB

:PERI-PARTUM

CHALLENGES围产期的Faecal

transit

regulation调控正常排便Optimal

farrowing

conditions最佳分娩条件Quality

of

newborn

piglet/

vitality

at

birth新生仔猪品质-出生Quality

of

newborn

piglet/

neonatal

diarrhoea新生仔猪品质-新生仔猪腹泻Reference:Customer

experimental

farm,France,2006.

法国，客户的试验猪场Animals:14

sows

initially.

14头母猪-Treatments:LEVUCELL®

SB

(2

x

106

CFU/g)

from

d85

of

gestation

vs.

Control.组从妊娠85天开始添加，配合料

活菌数为

2

x

106Method:

150

g

of

diatomeceous

earth

the

morning

before

moving

to

farrowing

house.Faecal

samples

collectedand ysed

twice

a

day.转产房当日饲喂150g硅藻土做指示剂；每天收集两次粪便样品进行分析。➥

Highly

variable

transit排便模式的差异很大➥

Regular

andhomogeneous

transit排便规律且全群呈现一致规律LEVUCELL®SB

:

IMPROVES

SOWS’TRANSIT

QUALITY

PERI-PARTUM.Discussion

分析

:➥

In

the

control

group,

one

sow

did

not

eat

any

feed

and

never

produced

any

faecalmaterial

during

the

eleven

days

of

the

trial.在对照组中，一头母猪在整个11天的试验期决绝采食且完全没有排便。➥

The

faecal

excretion

pattern

of

mineral

tracer

was

highly

variable

between

days

andbetween

sows

in

the

control

group.在对照组中，矿物质示踪剂的粪便排空模式每天呈现不同规律，且母猪间差异很大。➥

An

excretion

peak

of

mineral

tracer

could

be

observed

in

only

one

sow

out

of6in

the

control

group.在对照组中，6头母猪中只有一头呈现出矿物质示踪剂的排出峰值。➥

In

the

LEVUCELL®

SB

group,

a

common

/

homogeneous

excretion

patternwas

observed

for

all

7

sows

on

trial

with

an

excretion

peak

between

48

and

72

hours.7头 母猪呈现规律相同的排便模式，排便峰值出现在48~72小时之间。Conclusion

结论:LEVUCELL®

SB

improves

sows

transit

peri‐partum.改善母猪围产期便秘WHAT

DOES

«

OPTIMAL

FARROWING

CONDITIONS

»

MEAN?➥

Farrowing:

delivering

«

live

piglets

»

thanks

to

a

phase

of

muscular

contraction.分娩：强有力的节律性肌肉收缩确保母猪产“活仔”➥

Objectives:

Quick

&

Efficient

farrowing

process

to

ensure

good

quality

piglets.目标：快速且有效的分娩过程确保新生仔猪的健康状态良好➥Main

risksfor

the

sows:母猪的主要风险Too

long

/

Excessive

duration

➔

exhausted

sows.产程严重延长导致母猪精力衰竭Pain,

nervousness

➔

irregular

and

inefficient

uterine

contractions.疼痛和长时间紧张导致母猪

收缩不规律和效率不高➥Main

risks

for

the

piglets:仔猪的主要风险Hypoxia/Mechanical

trauma.组织缺氧/机械损伤Mortality.Weak

piglets.弱仔Lower

Vitality.

差Poor

colostrum

intake( ty/quality).初乳采食不理想LEVUCELL®SB

IMPROVES

FARROWING

CONDITIONSReference:Customer

experimental

farm,France,2006.法国，客户的实验猪场Animals:36

sows.

36头实验母猪Treatments:LEVUCELL®

SB(2

x

106

CFU/g)from

d85

of

gestation

vs.

Control.产前4周Method:farrowing

time

is

taken

after

the

piglet

is

born.产程从第一头仔猪出生开始计算LEVUCELL®

SB

decreases

the

risks

of

pathological

/

very

long

farrowing

(>

4hours).The

distribution

of

farrowingprocess

duration

is

mu ore

homogeneous

in

theLEVUCELL®

SBgroup

and

seems

to

be

correlated

with

litter

size.降低了病理性或超长产程（>4小时）的风险，产程与产仔数呈现相关性，产程分布规律。With

LEVUCELL®SB,

farrowing

process

is

more

efficient

and

homogeneous.HOW

TO

DEFINE

“PIGLETS

VITALITY

AT

BIRTH

“?Definition:➥

«Piglet

Vitality

at

birth»:

Pigl