小动物体内可见光检测

In

Vivo

Optical

ImagingDNA,

RNA,

Protein

CellsMiceTissue

HumanIn

VitroIn

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TechnologyStructural

ImagingComplicatedMRIOptical

ImagingPET/SPETUltrasoundCTSimpleFunctional

ImagingOptical

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ApplicationsOncologyStem

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Disease……Application:

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cancer

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Engraftmentand

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through

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measurement(tumor

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Cardiovascular

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Vivo

Visualization

of

Embryonic

Stem

Cell

Survival,Proliferation,

and

Migration

After

Cardiac

Diliveryinjected

intramyocardially

with

107

of

ES-TF

cellsApplication:

DNA

VaccineComplementary

Optical

Imaging

ApplicationsIn

VitroHTS

ScreeningColony

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BiologyEx

VivoHistological

Slidesan

ysisBeyond

Small

AnimalsBotanical

ModelsInsectsMonkeys,

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IVISThe

Leading

Platform

For

Optical

Imaging

SystemWhy

Do

More

Researchers

Use

IVIS?Patent

ownerSensitive

detection

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detection

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bioluminescence

and

fluorescenceSpectral

unmixing

for

multiple

reporter

detectionAutofluorescent

subtraction

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background

filtersTotal

solution,

not

only

a

instrumentMost

published

systemWidest

applicationIVIS

Performance

for

BioluminescenceBack-illuminated,

back-thinned,

-90C,

grade

1

CCD050100150200250-15-40CCD

temperature-3050403020100S/NSignal（105

photons）Noise（105

photons）-60

-90“This

approach

has

several

advantages

over

conventional

tumor

models:

the

sensitive

of

cell

detection

in

vivo

issurprisingly

highandexceeds

even

the

sensitivity

of

detection

by

flow

cytometry

ex

vivo.

As

few

as

7×103

cells

aredetectable

in

the

lungs

early

after

injection,

2-2.5

×104

cells

within

liver

or

spleen…

In

other

experiments

using

cells

with

even

higher

luciferase

expression,

as

few

as

100

cells

can

be

reliably

detected

in

the

peritoneal

ofliving

animals.

”

Since

cells

are

detectable

even

from

deep

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tissues,

tumor

cell

trafficking,

engraftment

indifferent ans,

and

metastasis

could

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visualized

without

perturbing

intact an

systems.------

Blood

2003,

15(101):640-648IVIS

Performance

for

BioluminescenceIVIS

system

calibrated

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absolutetationPhotons/sec/cm2/steradianNIST

traceable

source

for

calibrationComparation

between

experiments

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locationROI：Region

of

interestIVIS

Performance

for

FluorescenceIVIS

Performance

for

FluorescenceAutofluorescent

subtraction

using

background

filtersBackground

filterExcitation

filter-=1.4xSignal/Bkg

=

6.5Peak

signal/RMSerror

=

150IVIS

Performance

for

FluorescenceIVIS®

Lumina

II

：Powerful

Optical

Imaging-90C

Grade

1

CCD,

back-thinned,back-illuminated13

mm,

1024x1024,

f/0.95-f/16,

FOV=5-12.5cmSuperior

imaging

resultsBackground

filters10

exciting

filters

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common

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bioluminescence

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fluorescenceUpgraded

living

imaging

softwareIVIS®

Spectrum:

3D

Imaging

System3D

imaging

system-90C

Grade

1

CCD,

back-thinned,back-illuminated27

mm,

2048x2048,

f/0.95-f/8,

FOV=5-23cmTransmission

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emission

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unmixing

algorithmsWidest

Application,

Most

CustomerCustomers

in

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