各种激发光和吸收光波长

1、各种激发光和吸收光波长现在发现一个更全的有关激发光和发射光的波长图谱。这对CONFOCAL去做多重染色时是必不可少的参考资料。全部文件请到这里下载！Excitation and Emission Wavelengths of FluorophoresTavis FluoroTable: Common fluorophores (from Zeiss Corporation website)FluorophoreAbsorption EmissionOther info1,5 IAEDANS3364901,8-ANS3724804-Methylumbelliferone3855025-carbo

2、xy-2,7-dichlorofluorescein5045295-Carboxyfluorescein (5-FAM)4925185-Carboxynapthofluorescein (pH 10)512/598563/668Ratio Dye, pH5-Carboxytetramethylrhodamine542568(5-TAMRA)5-FAM (5-Carboxyfluorescein)4925185-HAT (Hydroxy Tryptamine)370-415520-5405-Hydroxy Tryptamine (HAT)370-415520-5405-ROX (carboxy-

3、X-rhodamine)5786045675915-TAMRA548552(5-Carboxytetramethylrhodamine)5425686-Carboxyrhodamine 6G5185436-CR 6G5185436-JOE5205487-Amino-4-methylcoumarin3514307-Aminoactinomycin D (7-AAD)5466477-Hydroxy-4-methylcoumarin360449, 4559-Amino-6-chloro-2-methoxyacridine412, 430471, 474ABQ344445Acid Fuchsin540

4、630ACMA412, 430471, 474(9-Amino-6-chloro-2-methoxyacridine)Acridine Orange + DNA502526Acridine Orange + RNA460650Acridine Orange, both DNA & RNA440-480520-650Acridine Red455-600560-680Acridine Yellow470550Acriflavin436520Acriflavin Feulgen SITSA355-425460Aequorin (Photoprotein)466PhotoproteinAFPs -

5、AutoFluorescent Protein - (QuantumBiotechnologies) see sgGFF, sgBFPAlexa Fluor 350346442342441Alexa Fluor 430431540Alexa Fluor 488495, 492519, 520Alexa Fluor 532531, 532553, 554Alexa Fluor 546556, 557572, 573Alexa Fluor 568577, 578603Alexa Fluor 594590, 594617, 618Alexa Fluor 633632650Alexa Fluor 64

6、7647666Alexa Fluor 660668698Alexa Fluor 680679702Alizarin Complexon530-560,580,580624-645Alizarin Red530-560580Allophycocyanin (APC)630, 645655, 660AMC, AMCA-S345445AMCA (Aminomethylcoumarin)345425347444AMCA-X353442Aminoactinomycin D555655Aminocoumarin346442350445Aminomethylcoumarin (AMCA)3454253474

7、44Anilin Blue600Anthrocyl stearate360-381446APC (Allophycocyanin)630, 645655, 660APC-Cy7625-650755APTRA-BTC = Ratio Dye, Zn2+466/380520/530 Ratio Dye, Zn2+APTS424505Astrazon Brilliant Red 4G500585Astrazon Orange R470540Astrazon Red 6B520595Astrazon Yellow 7 GLL450480Atabrine436490ATTO-TAG? CBQCA4655

8、60ATTO-TAG? FQ486591Auramine460550Aurophosphine G450580Aurophosphine450-490515BAO 9(Bisaminophenyloxadiazole)365395BCECF (high pH)492, 503520,528BCECF (low pH)482520Berberine Sulphate430550Beta Lactamase409447, 520BFP blue shifted GFP (Y66H)381,382,445, 447,blue shifted GFP (Y66H)Blue Fluorescent Pr

9、otein383448Blue Fluorescent ProteinBFP/GFP FRETBimane398490Bisbenzamide360461Bisbenzimide (Hoechst)360461bis-BTC = Ratio Dye, Zn2+455/405529/505Ratio Dye, Zn2+Blancophor FFG390470Blancophor SV370435BOBO?-1462481BOBO?-3570602Bodipy 492/515490515Bodipy 493/503533549Bodipy 500/510509515Bodipy 505/51550

10、2510Bodipy 530/550528547Bodipy 542/563543563Bodipy 558/568558569Bodipy 564/570564570Bodipy 576/589579590Bodipy 581/591584592Bodipy 630/650-X625642Bodipy 650/665-X647665Bodipy 665/676605676Bodipy Fl504, 505511, 513Bodipy FL ATP505514Bodipy Fl-Ceramide505511Bodipy R6G SE528547Bodipy TMR542574Bodipy TM

11、R-X conjugate544573Bodipy TMR-X, SE544570Bodipy TR589617Bodipy TR ATP591620Bodipy TR-X SE588616BO-PRO?-1462481BO-PRO?-3544570Brilliant Sulphoflavin FF430520BTC - Ratio Dye Ca2+464/401533/529Ratio Dye Ca2+BTC-5N - atio Dye, Zn2+459/417517/532Ratio Dye, Zn2+Calcein494517Calcein Blue373440Calcium Crims

12、on588, 589611, 615Calcium Green501, 506531Calcium Green-1 Ca2+ Dye506531Ca2+ DyeCalcium Green-2 Ca2+506/503536Ca2+Calcium Green-5N Ca2+506532Ca2+Calcium Green-C18 Ca2+509530Ca2+Calcium Orange549575576Calcofluor White385,395,437,440,405445Carboxy-X-rhodamine (5-ROX)576601Cascade Blue377420398423399Ca

13、scade Yellow399550400552Catecholamine410470CCF2 (GeneBlazer)CFDA494520CFP - Cyan Fluorescent Protein430,433,474,475,Cyan Fluorescent Protein436, (453)476, (501)CFP/YFP FRET Chlorophyll480650Chromomycin A436-460470Chromomycin A445575CL-NERF (Ratio Dye, pH)504/514540Ratio Dye, pHCMFDA494520Coelenteraz

14、ine Ca2+ Dye, bioluminescence (429)465Ca2+ Dye, bioluminescence,native moleculeCoelenterazine cp (Ca2+ Dye,)(430)442Ca2+ Dye, bioluminescenceCoelenterazine f(437)473Ca2+ Dye, bioluminescenceCoelenterazine fcp452Ca2+ Dye, bioluminescenceCoelenterazine h(437)464Ca2+ Dye, bioluminescenceCoelenterazine

15、hcp(433)444Ca2+ Dye, bioluminescenceCoelenterazine ip441Ca2+ Dye, bioluminescenceCoelenterazine n(431)467Ca2+ Dye, bioluminescenceCoelenterazine O460575Coumarin Phalloidin387470C-phycocyanineCPM Methylcoumarin384469MethylcoumarinCTC400-450602CTC FormazanCy2489506Cy3.1 8554568Cy3.5581598Cy35145665525

16、70554Cy5.1 8649666Cy5.5675695Cy5649666670Cy7710, 743767, 805Cyan GFP433 (453)475 (501)cyclic AMP Fluorosensor (FiCRhR)500517CyQuant Cell Proliferation Assay480520Cell Proliferation AssayDabcyl453Dansyl340578Dansyl Amine337517Dansyl Cadaverine335518Dansyl Chloride372518Dansyl DHPE336517Dansyl fluorid

17、e356noneDAPI359461Dapoxyl403580Dapoxyl 2374574Dapoxyl 3373574DCFDA504529DCFH(Dichlorodihydrofluorescein505535Diacetate)DDAO463607DHR (Dihydorhodamine 123)505534Di-4-ANEPPS496705Di-8-ANEPPS (non-ratio)488605498713DiA (4-Di-16-ASP)456591DichlorodihydrofluoresceinDiacetate505535(DCFH)DiD - Lipophilic T

18、racer644665Lipophilic TracerDiD (DiIC18(5)644665DIDS341415Dihydorhodamine 123 (DHR)505535Dil (DiIC18 (3)549, 551565Dinitrophenol349DiO (DiOC18(3)484, 487501, 502DiR748780Lipophilic TracerDiR (DiIC18(7)750779DM-NERF (high pH)497/510540Ratio Dye, pHDNP349Dopamine340490-520DsRed558583Red fluorescent pr

19、oteinDTAF494520DY-630-NHS621660Hemicyanelabel for proteins and DNADY-635-NHS634664Hemicyanelabel for proteins and DNAEBFP383447Enhanced BlueFluorescentProteinECFP436474Enhanced CyanFluorescentProteinEGFP488, 498507, 516Enhanced GreenFluorescentProteinELF 97345530Eosin524545Erythrosin529, 532554, 555

20、Erythrosin ITC529555Ethidium Bromide510, 523595, 605Ethidium homodimer -1 (EthD-1)528617EuchrysinEukoLightEuropium (III) chloride430540EYFP513, 520527, 532Enhanced YellowFluorescentProteinFast Blue360440FDA494520Feulgen (Pararosaniline)570625FIF (Formaldehyd Induced Fluorescence)405433FITC490, 49452

21、0, 525FITC Antibody493517Flazo Orange375-530612Fluo-3480 ?506,520, 527506Fluo-4494516Fluorescein (FITC)490, 494520, 525Fluorescein Diacetate494520Fluoro-Emerald495524Fluoro-Gold (Hydroxystilbamidine)361536Fluor-Ruby555582FluorX494520FM 1-43479598FM 4-46515640Fura Red?(high pH)Fura Red?Fluo-3572657Fu

22、ra-2, high calcium335505Excitation ratio dyeFura-2, low calcium363512Excitation ratio dyeFura-2/BCECFGenacryl Brilliant Red B520590Genacryl Brilliant Yellow 10GF430485Genacryl Pink 3G470583Genacryl Yellow 5GF430475GeneBlazer (CCF2)GFP (S65T)498516GFP red shifted (rsGFP)498516GFP wild type, non-UV excitation (wtGFP)475509GFP wild type, UV excitation (wtGFP)395509GFPuv385508Gloxalic Acid405460Granular Blue355425Haematoporphyrin530-560580Hoechst 33258345487Hoechst 33342347Filter sets installed on the Axiovert 200M:1) BrightfieldDIC:filter cube co