Aristolochic-acid-A-Aristolochic-acid-I-DataSheet-生命科学试剂-MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEAristolochic acid ACat. No.: HY-N0510CAS No.: 313-67-7Synonyms: Aristolochic acid I; TR 1736分式: CHNO分量: 341.27作靶点: NF-B作通路: NF-B储存式: 4C, protect from light* In solvent : -80C, 6 months; -20C, 1 month (protect fromlight)溶解性数据体外实验

2、 DMSO : 50 mg/mL (146.51 mM; Need ultrasonic)H2O : 40% PEG300 5% Tween-80 45% salineSolubility: 2.08 mg/mL (6.09 mM); Clear solutionBIOLOGICAL ACTIVITY1/2 Master of Small Molecules 您边的抑制剂师www.MedChemE物活性 Aristolochic acid A (Aristolochic acid I; TR 1736)是植物提取物 Aristolochic acids 的主要成分，其存在于Aristoloch

3、ia 和 Asarum 草本植物中。Aristolochic acid A 显 降低激活蛋1 (AP-1) 和 NF-B 活性。Aristolochic acid A 降低细胞中膀胱癌相关 BLCAP基因表达 1。体外研究 Aristolochic acid A (150, 200 M, 24hours) inhibits the cell viabilities of kidney cells HEK293 and HK-2 1.Aristolochic acid A (100, 200 M, 24hours) causes a concentration-dependent decreas

4、e in bladder cancer-associated protein (BLCAP) mRNA levels in kidney cells (HEK 293 and HK-2) , and bladder cancer cell line(HT-1376) 1.Aristolochic acid A (100, 200 M, 24hours) weakens the BLCAP protein signals in a dose-dependent mannerin both HEK293 and HT-1376 cells 1.Cell Viability Assay 1Cell

5、Line: Kidney cells (HEK 293 and HK-2)Concentration: 50, 100, 150, 200 MIncubation Time: 24 hoursResult: The cell viabilities of HEK293 and HK-2 were lower than that of vehicle-treated culturesuntil 150M and 200M.RT-PCR 1Cell Line: Kidney cells (HEK 293 and HK-2), and bladder cancer cell line (HT-137

6、6)Concentration: 100, 200 MIncubation Time: 24 hoursResult: Down regulated the levels of BLCAP mRNA.Western Blot Analysis 1Cell Line: HEK293 and HT-1376 cellsConcentration: 100, 200 MIncubation Time: 24 hoursResult: Reduced the BLCAP protein expression in a dose-dependent manner.REFERENCES1. Huang YT, et al. Aristolochic acid I interferes with the expression of BLCAP tumor suppressor gene in human cells. Toxicol Lett. 2018Jul;291:129-137.McePdfHeight2/2 Master of Small Molecules 您边的抑制剂师www.MedChemECaution: Product has not been fully validated for medical applications.For research use on