YK11 - Androgen Receptor Agonist - 生命科学试剂 - MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEYK11Cat. No.: HY-107480CAS No.: 1370003-76-1分式: CHO分量: 430.53作靶点: Androgen Receptor作通路: Others储存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性数据体外实验 DMSO : 64 mg/mL (148.65 mM)* means soluble, but satura

2、tion unknown.Mass Solvent1 mg 5 mg 10 mg Concentration制备储备液1 mM 2.3227 mL 11.6136 mL 23.2272 mL5 mM 0.4645 mL 2.3227 mL 4.6454 mL10 mM 0.2323 mL 1.1614 mL 2.3227 mL请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液，并请注意储备液的保存式和期限。BIOLOGICAL ACTIVITY物活性 YK11androgen 受体的部分激动剂，具有成活性。IC50 & Target Androgen receptor 1体外研究YK11

3、 is a partial agonist of androgen receptor, and induces myogenic differentiation of C2C12 Cells at 500nM via Fst mRNA upregulation by AR. YK11 enhances Myf5 and myogenin mRNA expression, but requires1/2 Master of Small Molecules 您边的抑制剂师www.MedChemEhigh concentrations (YK11, 100nM or 500nM) 1. YK11 (

4、0.5M) increases the cell growth of osteoblasticMC3T3-E1 cells, and enhances ALP activity via AR. YK11 also elevates osteoprotegerin mRNA expression(0.5M) and dose-dependently increases osteocalcin mRNA expression (0.1-1.0 M). In addition, YK11enhances phosphorylation of Akt protein via rapid non-gen

5、omic signaling 2.PROTOCOLCell Assay 1 Mouse myoblast C2C12 cells are cultured in Dulbeccos modified Eagles medium (DMEM) supplementedwith 10% fetal bovine serum (FBS) at 37C in a humidified atmosphere with 5% CO2. C2C12 cells areseeded on plates and maintained in culture medium for 24h. To induce my

6、ogenic differentiation, YK11 orDHT in DMEM supplemented with 2% horse serum (differentiation medium) is added to the cells on day 0.For the neutralization assay of Fst (also known as activin-binding protein), C2C12 cells are maintained indifferentiation medium in the presence of anti-Fst antibody 1.

7、MCE has not independently confirmed the accuracy of these methods. They are for reference only.REFERENCES1. Kanno Y, et al. Selective androgen receptor modulator, YK11, regulates myogenic differentiation of C2C12 myoblasts by follistatinexpression. Biol Pharm Bull. 2013;36(9):1460-5.2. Yatsu T, et al. Selective Androgen Receptor Modulator, YK11, Up-Regulates Osteoblastic Proliferation and Differentiation in MC3T3-E1Cells. Biol Pharm Bull. 2018;41(3):394-398.McePdfHeightCaution: Product has not been fully validated for medical applications.For re