Smac蛋白与肿瘤辐射敏感性#

1、中国科技论文在线- PAGE 6 - FORMTEXT Smac蛋白与肿瘤辐射敏感性基金项目：国家自然科学基金（30800281，31170804）；天津市自然科学基金重点项目（10JCZDJC16900，11ZCGYSY02400）；教育部高等学校博士学科点专项科研基金（200800231051，20101106110046）。 FORMTEXT 刘强1,2， FORMTEXT 杜利清1， FORMTEXT 曹嘉1， FORMTEXT 王彦1， FORMTEXT 王宏1， FORMTEXT 陈凤华1， FORMTEXT 付岳1， FORMTEXT 郭艳婷1， FORMTEXT 樊飞跃1作者简

2、介：刘强（1974-），男，研究员，主要从事放射生物学研究通信联系人：樊飞跃（1958-），男，研究员，主要从事放射生物学研究. E-mail: faithyfanSET version 1.5 * MERGEFORMAT1.5SET version 1.5 * MERGEFORMAT1.5SET version 1.5 * MERGEFORMAT1.5SET version 1.5 * MERGEFORMAT1.5SET version 1.5 * MERGEFORMAT1.5SET version 1.5 * MERGEFORMAT1.5SET version 1.5 * MERGEFOR

3、MAT1.5SET bkCompanyEN Institute of Radiation Medicine, Chinese Academy of Medical Science & Peking Union Medical College, Tianjin 300192;Institute of Radiation Medicine, Chinese Academy of Medical Science & Peking Union Medical College, Tianjin 300192;Institute of Radiation Medicine, Chinese Academy

4、 of Medical Science & Peking Union Medical College, Tianjin 300192;Institute of Radiation Medicine, Chinese Academy of Medical Science & Peking Union Medical College, Tianjin 300192;Institute of Radiation Medicine, Chinese Academy of Medical Science & Peking Union Medical College, Tianjin 300192;Ins

5、titute of Radiation Medicine, Chinese Academy of Medical Science & Peking Union Medical College, Tianjin 300192;Institute of Radiation Medicine, Chinese Academy of Medical Science & Peking Union Medical College, Tianjin 300192;Institute of Radiation Medicine, Chinese Academy of Medical Science & Pek

6、ing Union Medical College, Tianjin 300192;Institute of Radiation Medicine, Chinese Academy of Medical Science & Peking Union Medical College, Tianjin 300192 * MERGEFORMATInstitute of Radiation Medicine, Chinese Academy of Medical Science & Peking Union Medical College, Tianjin 300192;Institute of Ra

7、diation Medicine, Chinese Academy of Medical Science & Peking Union Medical College, Tianjin 300192;Institute of Radiation Medicine, Chinese Academy of Medical Science & Peking Union Medical College, Tianjin 300192;Institute of Radiation Medicine, Chinese Academy of Medical Science & Peking Union Me

8、dical College, Tianjin 300192;Institute of Radiation Medicine, Chinese Academy of Medical Science & Peking Union Medical College, Tianjin 300192;Institute of Radiation Medicine, Chinese Academy of Medical Science & Peking Union Medical College, Tianjin 300192;Institute of Radiation Medicine, Chinese

9、 Academy of Medical Science & Peking Union Medical College, Tianjin 300192;Institute of Radiation Medicine, Chinese Academy of Medical Science & Peking Union Medical College, Tianjin 300192;Institute of Radiation Medicine, Chinese Academy of Medical Science & Peking Union Medical College, Tianjin 30

10、0192SET bkCompanyCHN 中国医学科学院放射医学研究所；天津市分子核医学重点实验室，天津 300192;中国医学科学院放射医学研究所;中国医学科学院放射医学研究所;中国医学科学院放射医学研究所;中国医学科学院放射医学研究所;中国医学科学院放射医学研究所;中国医学科学院放射医学研究所;中国医学科学院放射医学研究所;中国医学科学院放射医学研究所 * MERGEFORMAT中国医学科学院放射医学研究所；天津市分子核医学重点实验室，天津 300192;中国医学科学院放射医学研究所;中国医学科学院放射医学研究所;中国医学科学院放射医学研究所;中国医学科学院放射医学研究所;中国医学科学院放

11、射医学研究所;中国医学科学院放射医学研究所;中国医学科学院放射医学研究所;中国医学科学院放射医学研究所SET bkPostcode 300192;300192 * MERGEFORMAT300192;300192SET bkMobile13672122168 * MERGEFORMA13672122168SET bkTelphone022-85683036 * MERGEFORMA022-85683036SET bkAddress 天津市南开区白堤路238号;天津市南开区白堤路238号

12、* MERGEFORMAT天津市南开区白堤路238号;天津市南开区白堤路238号SET bkEmail dr.qiangliu;jillpumc;bamboo19750930;biotechong;wddr0710;faithyfan * MERGEFORMATdr.qiangliu;jillpumc;bamboo19750930;biotechong;wddr0710;faithyfanSET bkIntroduction 刘强（1974-），男，研究员，主要从事放射生物学研究;樊飞跃（1958-），男，研究员，主要从事放射生物学研究 * MERGEFORMAT刘强（1974-），男，研究员

13、，主要从事放射生物学研究;樊飞跃（1958-），男，研究员，主要从事放射生物学研究SET bkAuthorCHN 刘强;杜利清;曹嘉;王彦;王宏;陈凤华;付岳;郭艳婷;樊飞跃 * MERGEFORMAT刘强;杜利清;曹嘉;王彦;王宏;陈凤华;付岳;郭艳婷;樊飞跃SET bkAuthorEN Liu Qiang;Du Liqing;Cao Jia;Wang Yan;Wang Hong;Chen Fenghua;Fu Yue;Guo Yanting;Fan Feiyue * MERGEFORMATLiu Qiang;Du Liqing;Cao Jia;Wang Yan;Wang Hong;Chen

14、 Fenghua;Fu Yue;Guo Yanting;Fan FeiyueSET bkContact 樊飞跃 * MERGEFORMAT樊飞跃SET bkFund 国家自然科学基金（30800281，31170804）；天津市自然科学基金重点项目（10JCZDJC16900，11ZCGYSY02400）；教育部高等学校博士学科点专项科研基金（200800231051，20101106110046）。 * MERGEFORMAT国家自然科学基金（30800281，31170804）；天津市自然科学基金重点项目（10JCZDJC16900，11ZCGYSY02400）；教育部高等学校博士学科点专

15、项科研基金（200800231051，20101106110046）。SET version 1.5 * MERGEFORMAT1.5SET version 1.5 * MERGEFORMAT1.5SET version 1.5 * MERGEFORMAT1.5SET version 1.5 * MERGEFORMAT1.5SET version 1.5 * MERGEFORMAT1.5SET version 1.5 * MERGEFORMAT1.5SET version 1.5 * MERGEFORMAT1.5SET version 1.5 * MERGEFORMAT1.5SET versi

16、on 1.5 * MERGEFORMAT1.5SET version 1.5 * MERGEFORMAT1.5SET version 1.5 * MERGEFORMAT1.5SET version 1.5 * MERGEFORMAT1.5SET bkReferencesInfo 1*|*期刊*|*褚丽萍，刘强，王芹等. mtDNA4977和彗星分析评价肿瘤细胞辐射敏感性研究J。中华放射医学与防护杂志，2008，28（2）：142-145。2*|*期刊*|*Chinnery PF, Samuels DC,Elson J, et al. Accumulation of mitochondrial

17、DNA mutations in ageing, cancer, and mitochondrial disease：is there a common mechanismJ? Lancet, 2002,360(9342):1323-1325.3*|*期刊*|*Kashkar H, Haefs C, Shin H, et al. XIAP-mediated caspase inhibition in Hodgkins lymphoma-derived B cellsJ. J Exp Med. 2003 Jul 21;198(2):341-347.4*|*期刊*|*DU C, Fang M, L

18、i Y, et a1. Smac，a mitochondrial protein that promotes cytochrome- dependent caspase activation by elimination IAP inhibitionJ. Ce11, 2000, l02(1):33-42.5*|*期刊*|*Verhagen AM, Ekert PG, Pakusch M, et a1. Identification of DIABLO, a mammalian protein that promotes apoptosis by binding to and antagoniz

19、ing IAP proteinsJ. Cell, 2000, 102(1):43-53.6*|*期刊*|*Giagkousiklidis S, Vogler M, Westhoff M, et al. Sensitization for irradiation induced apoptosis by second mitochondria-derived activator of caspaseJ. Cancer Res. 2005, 65(22): 10502-10513.7*|*期刊*|*Srinivasula SM, Hegde R, Saleh A, et al. A conserv

20、ed XIAP-interaction motif in caspase-9 and Smac/DIABLO regulates caspase activity and apoptosisJ. Nature. 2001, 410, 112-116.8*|*期刊*|*Liu Z, Sun C, Olejniczak ET,et al. Structural basis for binding of Smac/DIABLO to the XIAP BIR3 domainJ. Nature.2000, 408, 1004-1008.9*|*期刊*|*Srinivasula SM, Datta P,

21、 Fan XJ, et al. Molecular determinants of the caspase-promoting activity of Smac/DIABLO and its role in the death receptor pathwayJ. J Biol Chem . 2000, 275, 36152-36157.10*|*期刊*|*Deveraux QL, Takahashi R, Salvesen GS, et a1. X-linked IAP is a direct inhibitor of cel1-death proteasesJ. Nature, 1997,

22、 388(6639): 300-304.11*|*期刊*|*Vucic D, Deshayes K, Ackerly H, et a1. SMAC negatively regulates the anti-apoptotic activity of melanoma inhibitor of apoptosis (ML-IAP)J. J Biol Chem. 2002 , 277(14):12275-12279.12*|*期刊*|*Jia L, Patwari Y, Kelsey SM, et a1. Role of Smac in human leukaemic cell apoptosi

23、s and proliferationJ. Oncogene. 2003, 22(11):1589-1599.13*|*期刊*|*Chai J, Du C, Wu JW, et al. Structural and biochemical basis of apoptotic activation by Smac/DIABLOJ. Nature. 2000, 406, 855-862.14*|*期刊*|*Sohn D, Schulze-Osthoff K, Janicke RU. Caspase-8 can be activated by interchain proteolysis with

24、out receptor triggered dimerization during drug-induced apoptosisJ. J Biol Chem. 2005,8: 5267-5273.15*|*期刊*|*Guo F, Nimmanapalli R, hanthi Paranawithana S, et al. Ectopic overexpression of second mitochondria-derived activator of caspases (Smac/DIABLO) or cotreatment with N-terminus of Smac/DIABLO p

25、eptide potentiates epothilone B derivative(BMS 247550) and Apo-2L/TRAILinduced apoptosisJ. Blood, 2002, 99: 3419-3426.16*|*期刊*|*Yang L, Mashima T, Sato S, et al. Predominant Suppression of Apoptosome by Inhibitor of Apoptosis Protein in Non-Small Cell Lung Cancer H460 Cells: Therapeutic Effect of a

26、Novel Polyarginine-conjugated Smac PeptideJ. Cancer Res. 2003, 63: 831-837.17*|*期刊*|*Derossi D, Calvet S, Trembleau A, et al. Cell internalization of the third helix of the Antennapedia homeodomain is receptor- independentJ. J Biol Chem, 1996, 271(30):18188-1819318*|*期刊*|*Yang J, McEachern D, Li W,

27、et al. Radiosensitization of head and neck squamous cell carcinoma by a SMAC-mimetic compound, SM-164, requires activation of caspasesJ. Mol Cancer Ther. 2011 10(4):658-69.19*|*期刊*|*Schmidt N, Mishra A, Lai GH, et al. Arginine-rich cell-penetrating peptidesJ. FEBS Letters. 2010, 584:1806-1813.20*|*期

28、刊*|*Kamide K, Nakakubo H, Uno S, Fukamizu A. Isolation of novel cell-penetrating peptides from a random peptide library using in vitro virus and their modificationsJ. Int J Mol Med. 2010; 25(1): 41-51.21*|*期刊*|*Lim KS, Won YW, Park YS, Kim YH. Preparation and functional analysis of recombinant prote

29、in transduction domain-metallothionein fusion proteinsJ. Biochimie. 2010,92(8):964-70.22*|*期刊*|*Huerta S, Gao X, Livingston EH, et al. In vitro and in vivo radiosensitization of colorectal cancer HT-29 cells by the smac mimetic JP-1201J. Surgery. 2010 Aug;148(2):346-53.23*|*期刊*|*Cheung HH, Beug ST,

30、St Jean M, et al. Smac mimetic compounds potentiate interleukin-1 beta-mediated cell deathJ. J Biol Chem. 2010; 285(52):40612-23.24*|*期刊*|*Petersen SL, Peyton M, Minna JD, Wang X. Overcoming cancer cell resistance to Smac mimetic induced apoptosis by modulating cIAP-2 expressionJ. Proc Natl Acad Sci

31、 U S A. 2010; 107(26): 11936-41.25*|*期刊*|*Servida F, Lecis D, Scavullo C, et al. Novel second mitochondria-derived activator of caspases (Smac) mimetic compounds sensitize human leukemic cell lines to conventional chemotherapeutic drug-induced and death receptor-mediated apoptosis. Invest New DrugsJ

32、. 2011, 29(6):1264-75.26*|*期刊*|*Probst BL, Liu L, Ramesh V, et al. Smac mimetics increase cancer cell response to chemotherapeutics in a TNF-dependent mannerJ. Cell Death Differ. 2010; 17(10):1645-54.27*|*期刊*|*Hosokawa Y, Sakakura Y, Tanaka L, Okumura K, Yajima T, Kaneko M. Radiation-induced apoptos

33、is is independent of caspase-8 but dependent on cytochrome c and the caspase-9 cascade in human leukemia HL60 cellsJ. J Radiat Res, 2005;46(3):293-303. * MERGEFORMAT1*|*期刊*|*褚丽萍，刘强，王芹等. mtDNA4977和彗星分析评价肿瘤细胞辐射敏感性研究J。中华放射医学与防护杂志，2008，28（2）：142-145。2*|*期刊*|*Chinnery PF, Samuels DC,Elson J, et al. Accum

34、ulation of mitochondrial DNA mutations in ageing, cancer, and mitochondrial disease：is there a common mechanismJ? Lancet, 2002,360(9342):1323-1325.3*|*期刊*|*Kashkar H, Haefs C, Shin H, et al. XIAP-mediated caspase inhibition in Hodgkins lymphoma-derived B cellsJ. J Exp Med. 2003 Jul 21;198(2):341-347

35、.4*|*期刊*|*DU C, Fang M, Li Y, et a1. Smac，a mitochondrial protein that promotes cytochrome- dependent caspase activation by elimination IAP inhibitionJ. Ce11, 2000, l02(1):33-42.5*|*期刊*|*Verhagen AM, Ekert PG, Pakusch M, et a1. Identification of DIABLO, a mammalian protein that promotes apoptosis by

36、 binding to and antagonizing IAP proteinsJ. Cell, 2000, 102(1):43-53.6*|*期刊*|*Giagkousiklidis S, Vogler M, Westhoff M, et al. Sensitization for irradiation induced apoptosis by second mitochondria-derived activator of caspaseJ. Cancer Res. 2005, 65(22): 10502-10513.7*|*期刊*|*Srinivasula SM, Hegde R,

37、Saleh A, et al. A conserved XIAP-interaction motif in caspase-9 and Smac/DIABLO regulates caspase activity and apoptosisJ. Nature. 2001, 410, 112-116.8*|*期刊*|*Liu Z, Sun C, Olejniczak ET,et al. Structural basis for binding of Smac/DIABLO to the XIAP BIR3 domainJ. Nature.2000, 408, 1004-1008.9*|*期刊*|

38、*Srinivasula SM, Datta P, Fan XJ, et al. Molecular determinants of the caspase-promoting activity of Smac/DIABLO and its role in the death receptor pathwayJ. J Biol Chem . 2000, 275, 36152-36157.10*|*期刊*|*Deveraux QL, Takahashi R, Salvesen GS, et a1. X-linked IAP is a direct inhibitor of cel1-death

39、proteasesJ. Nature, 1997, 388(6639): 300-304.11*|*期刊*|*Vucic D, Deshayes K, Ackerly H, et a1. SMAC negatively regulates the anti-apoptotic activity of melanoma inhibitor of apoptosis (ML-IAP)J. J Biol Chem. 2002 , 277(14):12275-12279.12*|*期刊*|*Jia L, Patwari Y, Kelsey SM, et a1. Role of Smac in huma

40、n leukaemic cell apoptosis and proliferationJ. Oncogene. 2003, 22(11):1589-1599.13*|*期刊*|*Chai J, Du C, Wu JW, et al. Structural and biochemical basis of apoptotic activation by Smac/DIABLOJ. Nature. 2000, 406, 855-862.14*|*期刊*|*Sohn D, Schulze-Osthoff K, Janicke RU. Caspase-8 can be activated by in

41、terchain proteolysis without receptor triggered dimerization during drug-induced apoptosisJ. J Biol Chem. 2005,8: 5267-5273.15*|*期刊*|*Guo F, Nimmanapalli R, hanthi Paranawithana S, et al. Ectopic overexpression of second mitochondria-derived activator of caspases (Smac/DIABLO) or cotreatment with N-

42、terminus of Smac/DIABLO peptide potentiates epothilone B derivative(BMS 247550) and Apo-2L/TRAILinduced apoptosisJ. Blood, 2002, 99: 3419-3426.16*|*期刊*|*Yang L, Mashima T, Sato S, et al. Predominant Suppression of Apoptosome by Inhibitor of Apoptosis Protein in Non-Small Cell Lung Cancer H460 Cells:

43、 Therapeutic Effect of a Novel Polyarginine-conjugated Smac PeptideJ. Cancer Res. 2003, 63: 831-837.17*|*期刊*|*Derossi D, Calvet S, Trembleau A, et al. Cell internalization of the third helix of the Antennapedia homeodomain is receptor- independentJ. J Biol Chem, 1996, 271(30):18188-1819318*|*期刊*|*Ya

44、ng J, McEachern D, Li W, et al. Radiosensitization of head and neck squamous cell carcinoma by a SMAC-mimetic compound, SM-164, requires activation of caspasesJ. Mol Cancer Ther. 2011 10(4):658-69.19*|*期刊*|*Schmidt N, Mishra A, Lai GH, et al. Arginine-rich cell-penetrating peptidesJ. FEBS Letters. 2

45、010, 584:1806-1813.20*|*期刊*|*Kamide K, Nakakubo H, Uno S, Fukamizu A. Isolation of novel cell-penetrating peptides from a random peptide library using in vitro virus and their modificationsJ. Int J Mol Med. 2010; 25(1): 41-51.21*|*期刊*|*Lim KS, Won YW, Park YS, Kim YH. Preparation and functional anal

46、ysis of recombinant protein transduction domain-metallothionein fusion proteinsJ. Biochimie. 2010,92(8):964-70.22*|*期刊*|*Huerta S, Gao X, Livingston EH, et al. In vitro and in vivo radiosensitization of colorectal cancer HT-29 cells by the smac mimetic JP-1201J. Surgery. 2010 Aug;148(2):346-53.23*|*

47、期刊*|*Cheung HH, Beug ST, St Jean M, et al. Smac mimetic compounds potentiate interleukin-1 beta-mediated cell deathJ. J Biol Chem. 2010; 285(52):40612-23.24*|*期刊*|*Petersen SL, Peyton M, Minna JD, Wang X. Overcoming cancer cell resistance to Smac mimetic induced apoptosis by modulating cIAP-2 expres

48、sionJ. Proc Natl Acad Sci U S A. 2010; 107(26): 11936-41.25*|*期刊*|*Servida F, Lecis D, Scavullo C, et al. Novel second mitochondria-derived activator of caspases (Smac) mimetic compounds sensitize human leukemic cell lines to conventional chemotherapeutic drug-induced and death receptor-mediated apo

49、ptosis. Invest New DrugsJ. 2011, 29(6):1264-75.26*|*期刊*|*Probst BL, Liu L, Ramesh V, et al. Smac mimetics increase cancer cell response to chemotherapeutics in a TNF-dependent mannerJ. Cell Death Differ. 2010; 17(10):1645-54.27*|*期刊*|*Hosokawa Y, Sakakura Y, Tanaka L, Okumura K, Yajima T, Kaneko M.

50、Radiation-induced apoptosis is independent of caspase-8 but dependent on cytochrome c and the caspase-9 cascade in human leukemia HL60 cellsJ. J Radiat Res, 2005;46(3):293-303.SET bkAuthorsInfo |1|刘强|Liu Qiang|中国医学科学院放射医学研究所；天津市分子核医学重点实验室，天津 300192|Institute of Radiation Medicine, Chinese Academy of

51、 Medical Science & Peking Union Medical College, Tianjin 300192|刘强（1974-），男，研究员，主要从事放射生物学研究|天津市南开区白堤路238号|300192|dr.qiangliu13612072983|2|杜利清|Du Liqing|中国医学科学院放射医学研究所|Institute of Radiation Medicine, Chinese Academy of Medical Science & Peking Union Medical College, Tianjin 300192|3|曹嘉

52、|Cao Jia|中国医学科学院放射医学研究所|Institute of Radiation Medicine, Chinese Academy of Medical Science & Peking Union Medical College, Tianjin 300192|jillpumc|4|王彦|Wang Yan|中国医学科学院放射医学研究所|Institute of Radiation Medicine, Chinese Academy of Medical Science & Peking Union Medical College, Tianjin 300192|bamboo19

53、750930|5|王宏|Wang Hong|中国医学科学院放射医学研究所|Institute of Radiation Medicine, Chinese Academy of Medical Science & Peking Union Medical College, Tianjin 300192|biotechong|6|陈凤华|Chen Fenghua|中国医学科学院放射医学研究所|Institute of Radiation Medicine, Chinese Academy of Medical Science & Peking Union Medical College, Tia

54、njin 300192|7|付岳|Fu Yue|中国医学科学院放射医学研究所|Institute of Radiation Medicine, Chinese Academy of Medical Science & Peking Union Medical College, Tianjin 300192|wddr0710|8|郭艳婷|Guo Yanting|中国医学科学院放射医学研究所|Institute of Radiation Medicine, Chinese Academy of Medical Science & Peking Union Medical College, Tian

55、jin 300192|*|9|樊飞跃|Fan Feiyue|中国医学科学院放射医学研究所|Institute of Radiation Medicine, Chinese Academy of Medical Science & Peking Union Medical College, Tianjin 300192|樊飞跃（1958-），男，研究员，主要从事放射生物学研究|天津市南开区白堤路238号|300192|faithyfan13672122168 * MERGEFORMAT|1|刘强|Liu Qiang|中国医学科学院放射医学研究所；天津市分子核医学重点实

56、验室，天津 300192|Institute of Radiation Medicine, Chinese Academy of Medical Science & Peking Union Medical College, Tianjin 300192|刘强（1974-），男，研究员，主要从事放射生物学研究|天津市南开区白堤路238号|300192|dr.qiangliu13612072983|2|杜利清|Du Liqing|中国医学科学院放射医学研究所|Institute of Radiation Medicine, Chinese Academy of Med

57、ical Science & Peking Union Medical College, Tianjin 300192|3|曹嘉|Cao Jia|中国医学科学院放射医学研究所|Institute of Radiation Medicine, Chinese Academy of Medical Science & Peking Union Medical College, Tianjin 300192|jillpumc|4|王彦|Wang Yan|中国医学科学院放射医学研究所|Institute of Radiation Medicine, Chinese Academy of Medical

58、 Science & Peking Union Medical College, Tianjin 300192|bamboo19750930|5|王宏|Wang Hong|中国医学科学院放射医学研究所|Institute of Radiation Medicine, Chinese Academy of Medical Science & Peking Union Medical College, Tianjin 300192|biotechong|6|陈凤华|Chen Fenghua|中国医学科学院放射医学研究所|Institute of Radiation Medicine, Chines

59、e Academy of Medical Science & Peking Union Medical College, Tianjin 300192|7|付岳|Fu Yue|中国医学科学院放射医学研究所|Institute of Radiation Medicine, Chinese Academy of Medical Science & Peking Union Medical College, Tianjin 300192|wddr0710|8|郭艳婷|Guo Yanting|中国医学科学院放射医学研究所|Institute of Radiation Medicine, Chinese Academy of Medical Science & Peking Union Medical College, Tianjin 300192|*|9|樊飞跃|Fan Feiyue|中国医学科学院放射医学研究所|Institute of Radiation Medicine, Chinese Academy of Medical Science & Peking Union Medical College, Tianjin 300192|樊飞跃（1958-），男，研究员，主要从事放射生物学研究|天津市南开区白堤路238号|300192|faithyfan|022-85