Protocatechuic-acid-3-4-Dihydroxybenzoic-acid-DataSheet-生命科学试剂-MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEProtocatechuic acidCat. No.: HY-N0294CAS No.: 99-50-3Synonyms: 3,4-Dihydroxybenzoic acid分式: CHO分量: 154.12作靶点: Others作通路: Others储存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性数据体外实验 DMSO : 50 mg/mL (324.

2、42 mM; Need ultrasonic)H2O : 10 mg/mL (64.88 mM; Need ultrasonic)Mass Solvent1 mg 5 mg 10 mg Concentration制备储备液1 mM 6.4885 mL 32.4423 mL 64.8845 mL5 mM 1.2977 mL 6.4885 mL 12.9769 mL10 mM 0.6488 mL 3.2442 mL 6.4885 mL请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液，并请注意储备液的保存式和期限。体内实验请根据您的实验动物和给药式选择适当的溶解案，配制前请先配制澄清的储备液

3、，再依次添加助溶剂(为保证实验结果的可靠性，体内实验的作液，建议您现现配，当天使；澄清的储备液可以根据储存条件，适当保存；以下溶剂前的百分 指该溶剂在您配制终溶液中的体积占)：1. 请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% salineSolubility: 2.5 mg/mL (16.22 mM); Clear solution2. 请依序添加每种溶剂： 10% DMSO 90% (20% SBE-CD in saline)Solubility: 2.5 mg/mL (16.22 mM); Clear solution1/3 Master

4、of Small Molecules 您边的抑制剂师www.MedChemE3. 请依序添加每种溶剂： 10% DMSO 90% corn oilSolubility: 2.5 mg/mL (16.22 mM); Clear solutionBIOLOGICAL ACTIVITY物活性 Protocatechuic acid种天然多酚类物质，具有神经保护的作。体外研究 Protocatechuic acid inhibits the aggregation of A and S and destabilizes their preformed fibrils.Protocatechuic ac

5、id prevents the death of PC12 cells triggered by A- and S-induced toxicity 3.体内研究 Protocatechuic acid is able to prevent stress induced immobility time in forced swim test without alteringlocomotor activity in mice. Further, Protocatechuic acid treatment attenuates the elevation of serumcorticostero

6、ne, lipid peroxidation and restores enzymatic antioxidants in cerebral cortex and hippocampus inARS mice 1. Rat administered cadmium and treated with prostigmine and doses of Protocatechuic acid(1020 mg/kg) has significantly reduced BChE activity. Cadmium and either prostigmine or Protocatechuicacid

7、 (1020 mg/kg) treated rats shows significant reduction in MDA level 2.PROTOCOLKinase Assay 2 AChE activity investigation is carried out in a reaction mixture containing 50 L of tissue homogenate, 50 Lof 5, 5-dithiobis-(2-nitrobenzoic) acid (DTNB), 1175 L of 0.1 M phosphate-buffered solution, pH 8.0.

8、 Afterincubation for 20 min at 25C, 25 L of acetylthiocholine iodide solution is added as the substrate. The AChEactivity is determined as changes in absorbance reading at 412 nm for 3 min at 25C and using a UV/Visiblespectrophotometer.MCE has not independently confirmed the accuracy of these method

9、s. They are for reference only.Cell Assay 3 Dilutions of Protocatechuic acid (2, 5, 10, 20, 50, and 100 M) are prepared from stock solutions, with serum-free culture medium. Equal volumes of each solution are mixed with A1-42 (10 M), then incubated for 24 hon a thermoblock, with continuous agitation

10、, and then exposed to PC12 cells for 24 h to test whetherProtocatechuic acid can prevent cell death triggered by A. Cell viability is determined by MTT reductionassay. Cells are treated with 200 L per well of MTT solution (final concentration, 0.5 mg/mL in DMEM-Glutamax medium) for 3 h, at 37C, with

11、 5% CO2. The dark blue formazan crystals that formed aresolubilized with 100 L per well of DMSO, for 30 min. Absorbance is measured at 540 nm, with a microplatereader. Results are expressed as the percentage of MTT reduction in relation to the absorbance of controlcells at 100%.MCE has not independe

12、ntly confirmed the accuracy of these methods. They are for reference only.户使本产品发表的科研献 J Neurosci Res. 2019 Aug 16. Biochem Biophys Res Commun. 2018 Sep 3;503(1):297-303.2/3 Master of Small Molecules 您边的抑制剂师www.MedChemESee more customer validations on HYPERLINK / www.MedChemEREFERENCES1. Thakare VN,

13、et al. Attenuation of acute restraint stress-induced depressive like behavior and hippocampal alterations withprotocatechuic acid treatment in mice. Metab Brain Dis. 2016 Oct 26.2. Adefegha SA, et al. Alterations of Na+/K+-ATPase, cholinergic and antioxidant enzymes activity by protocatechuic acid in cadmium-induced neurotoxicity and oxidative stress in Wistar rats. Biomed Pharmacother. 2016 Oct;83:559-568.3. Hornedo-Ortega R, et al. Protocatechuic Acid: Inhibition of Fibril Formation, Destabilization of Preformed Fibrils of Amyloid- and -Synuclein, and Neuroprotection. J Agric Food Chem. 201